Dielectric analysis of changes in electric properties of leukemic cells through travelling and negative dielectrophoresis with 2-D electrodes
01 May 2017-pp 1-4
TL;DR: The dielectrophoretic separation in a microfluidic device based on 2D electrodes is studied and a comparison between the velocity of B and T leukemia K365 cells due to the drag force is conducted and presented.
Abstract: Dielectrophoretic force has been used to manipulate biological microparticles, such as red blood cells, white blood cells, cancer cells, etc… This technique has been used for trapping, sorting and separating biological particles suspended in a buffer medium The strength of dielectrophoretic force depends strongly on the particle's electrical properties, the geometry of the microfluidic device, dielectric properties of the medium, particle shape and size and the frequency of the electric field Therefore, to design an effective microfluidic separation platform, it is important to understand the effect and the role of the aforementioned parameters on the particle's motion In this paper, the dielectrophoretic separation in a microfluidic device based on 2D electrodes is studied Also, a comparison between the velocity of B and T leukemia K365 cells due to the drag force is conducted and presented Moreover, the conductance of the leukemia K365 cells before and after dragging is presented and discussed
Citations
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TL;DR: In this article, the effect of microelectrodes configuration on the sensitivity and accuracy of the micro-electrical impedance spectroscopy (μEIS) was demonstrated, fabricated and experimentally used to extract the electrical parameters of Human Bone Osteosarcoma Epithelial (U2OS) viable and dead cells.
Abstract: This paper demonstrates the effect of microelectrodes configuration on the sensitivity and accuracy of the micro-electrical impedance spectroscopy (μEIS). Bi-polar and tri-polar microelectrodes configurations were presented, fabricated and experimentally used to extract the electrical parameters of Human Bone Osteosarcoma Epithelial (U2OS) viable and dead cells. A comparison between the experimental results are presented and discussed. These experimental results show that the tri-polar electrode configuration has higher sensitivity compared to bi-polar configuration.
7 citations
27 May 2018
TL;DR: Studies of conductivity in a microfluidic device based on a 2-D electrode system and a comparison between the conductivity of leukemia k365, B-cell, and T-cell due to the drag and electrorotaional forces are presented and discussed.
Abstract: Electroporation is a powerful technique used to improve the permeability of the cell membrane; pores are created in the cell membrane based on the application of a high voltage. The high voltage improves the number of membrane pores, however, it might kill the cells. Electroporation is achieved using a microelectrode placed in a microfluidic device. This paper focuses on the improvement of the electroporation of a cell based on levitation dielectrophoresis and an electrorotation force. Then, the change of the conductivity is detected at both the frequency and time domain, i.e., individual cells are trapped due to the effect of levitation and electrorotation force, and then the variation in the conductivity is detected. We use frequency and time domain spectroscopy to measure the conductivity of the white blood cell, i.e., B-cell, T-cell and leukemia K365. In this paper, we review studies of conductivity separation in a microfluidic device based on a 2-D electrode system. Also, a comparison between the conductivity of leukemia k365, B-cell, and T-cell due to the drag and electrorotaional forces are presented and discussed.
4 citations
Cites methods from "Dielectric analysis of changes in e..."
...Electrical impedance spectroscopy (EIS) is considered a noninvasive method used to analyze single cell, according to their dielectric properties as a function of frequency [11]....
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01 Dec 2019
TL;DR: The difference in the electrical parameters e.g. Impedance, capacitance and permittivity were investigated for different cells such as the dead and viable Normal Human Bone Osteosarcoma Epithelial (U2OS) Cells at different frequencies using micro-electrical impedance spectroscopy.
Abstract: This work aims to investigate the difference in the electrical parameters e.g. Impedance, capacitance and permittivity. Those parameters were investigated for different cells such as the dead and viable Normal Human Bone Osteosarcoma Epithelial (U2OS) Cells at different frequencies using micro-electrical impedance spectroscopy $(\mu \mathrm{EIS}). >\mu \mathrm{EIS}$ defines the electrical parameters of normal U2OS cells inserted between sensing microelectrodes. The frequencies of the spectrum used to define the electrical parameters ranges from 1kHz to 3MHz.
2 citations
Cites background from "Dielectric analysis of changes in e..."
...It also correlates with different diseases such as cancer[2]....
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15 Dec 2020
TL;DR: In this article, a new methodology based on the combination between the dielectrophoresis response and the electric impedance detection was established to achieve the main target of the developing the cell detection techniques.
Abstract: Dielectrophoresis (DEP) force has established to be one of the strongest techniques can be used to control in the different biological cell using different techniques for example Cell sorting, Cell Transporting and Cell Trapping. The major target and challenge must be achieved are that how can improve and enhance the sensing and detection stage to enhance the overall detection method. In this study, we establish a new methodology based on the combination between the dielectrophoresis response and the electric impedance detection this combination can achieve the main target of the develop the cell detection techniques. The dielectrophoresis response can be extracted as a significant effect on the cell trapping under different force distribution. The force distribution and the cell response can be combined to enhance the biological cell and different particles identification and detection. The previous and the conventional studies on the cell detection ignore the effect of the cell distribution on the sensing and measuring electrodes during the dielectric parameters detection and measurements such as the ignoring of the cell distribution during the cell impedance detection. The random spread of the cells on the sensing electrodes consider a vital factor during cell dielectric parameters analysis. So, we focus on the using of the effect dielectrophoresis force can overcome this random cell distribution and make the cells being focused on the sensing electrodes.
References
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TL;DR: Findings indicate that the dielectric affinity technique may prove useful in a wide variety of cell separation and characterization applications.
Abstract: Electrorotation measurements were used to demonstrate that the dielectric properties of the metastatic human breast cancer cell line MDA231 were significantly different from those of erythrocytes and T lymphocytes. These dielectric differences were exploited to separate the cancer cells from normal blood cells by appropriately balancing the hydrodynamic and dielectrophoretic forces acting on the cells within a dielectric affinity column containing a microelectrode array. The operational criteria for successful particle separation in such a column are analyzed and our findings indicate that the dielectric affinity technique may prove useful in a wide variety of cell separation and characterization applications.
715 citations
TL;DR: Several interesting side effect phenomena coincident with nonuniform electric field conditions were observed, including stirring (related to "jet" effects at localized electrode sites), discontinuous repulsions, and cellular rotation which was found to be frequency dependent.
288 citations
"Dielectric analysis of changes in e..." refers background in this paper
...DEP was first reported by Debye Etalin 1954 [1]....
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TL;DR: It is concluded that MEMS-based components might contribute to some components in a sample-preparation system consisting of modular instruments and disposable units, but will not provide a generic or a totally integrated solution.
Abstract: Completion of the Human Genome Project is driving the rapid development of molecular diagnostics in the laboratory. To accelerate the penetration of genetic tests and other nucleic acid-based tests into clinical markets, simple, compact, automatic sample-preparation systems for molecular diagnostics must be developed. Microelectromechanical systems (MEMS) is a promising approach for the development of automated sample preparation for the clinical laboratory or point-of-care setting. This review discusses MEMS-based components that could be applied to the different stages of the sample-preparation process such as cell separation, nucleic acid purification, and nucleic acid amplification. Examples of functional component integration are given. Issues discussed include partitioning of functions between the instrument and disposable unit, methods of propulsion of fluids and particles, vapor and liquid barriers, and sample size. Although further evaluation and development are needed to provide practical solutions to some of these issues, we conclude that MEMS-based components might contribute to some components in a sample-preparation system consisting of modular instruments and disposable units, but will not provide a generic or a totally integrated solution.
250 citations
"Dielectric analysis of changes in e..." refers background in this paper
...The development of microfluidic devices for biomedical application has resulted in renewed interest in the DEP phenomena especially for applications such as cell trapping or separation [2]....
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TL;DR: The dielectric permittivity, capacitance and conductivity values of cell membranes are higher for normal lymphocytes than for the malignant ones and the difference of the same parameters for normal B and T-cells is discussed.
222 citations
"Dielectric analysis of changes in e..." refers background or methods in this paper
...The analysis is conducted for leukemia, T-cells and, B-cells (10 μm) with specific properties are as [9]....
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...Fig.8 shows the difference in the drag velocity for leukemia, T-cells and, B-cells....
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...As shown, the drag velocity of leukemia K365 is greater than the velocity of T-cells and B-cells....
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...1, for particle has been modeled based on n-DEP using COMSOL Multiphysics and the used parameters for medium are (water =80, =2* ), the copper was selected as electrode materials whereas the FR-4 is selected for the substrate material and cells are listed in [9]....
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...This property is clear if we compare the drag velocity of leukemia K365 with T-cells and B-cells [9]....
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TL;DR: Suspensions of yeast cells were used as model systems to investigate the electrokinetic behaviour of colloidal particles subjected to travelling electric fields generated using microelectrodes and dielectrophoresis and electrorotation measurements were made to provide a further understanding of the observed effects.
Abstract: Suspensions of yeast cells (Saccharomyces cerevisiae) were used as model systems to investigate the electrokinetic behaviour of colloidal particles subjected to travelling electric fields generated using microelectrodes. Measurements were made over the frequency range 1 kHz to 10 MHz and for suspending medium conductivities in the range 6-260 mS m-1. A theoretical model is developed to provide a good description of the dependence of the observed translational motion, termed travelling-wave dielectrophoresis (TWD), on the dielectric properties of the particle and suspending medium, on the size of the particle, and on the magnitude and frequency of the applied field. Unlike conventional dielectrophoresis, the TWD effect is found to be related to the imaginary, rather than to the real, component of the induced dipole moment. Dielectrophoresis and electrorotation measurements were made to provide a further understanding of the observed effects and to support the theoretical model.
168 citations
"Dielectric analysis of changes in e..." refers methods in this paper
...The proposed system combines the tw-DEP force for pushing the cells from the inlet toward the outlet, drag force using n-DEP to push the cells toward the detector area at the lower substrate [4-7]....
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...The cells are injected into the microfluidic chamber using tw-DEP electrodes instead of the mechanical injection [7]....
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