Differential MicroRNA Expression in Experimental Cerebral and Noncerebral Malaria
Fatima El-Assaad,Casper Hempel,Casper Hempel,Valery Combes,Andrew J. Mitchell,Helen J. Ball,Jørgen A. L. Kurtzhals,Nicholas H. Hunt,Jean Marie Mathys,Georges E. Grau +9 more
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TLDR
The data suggest that in the CBA mouse at least, miRNA may have a regulatory role in the pathogenesis of severe malaria.Abstract:
MicroRNAs (miRNAs) are posttranscriptional regulatory molecules that have been implicated in the regulation of immune responses, but their role in the immune response to Plasmodium infection is unknown. We studied the expression of selected miRNAs following infection of CBA mice with Plasmodium berghei ANKA (PbA), which causes cerebral malaria (CM), or Plasmodium berghei K173 (PbK), which causes severe malaria but without cerebral complications, termed non-CM. The differential expression profiles of selected miRNAs (let-7i, miR-27a, miR-150, miR-126, miR-210, and miR-155) were analyzed in mouse brain and heart tissue by quantitative reverse transcription-PCR (qRT-PCR). We identified three miRNAs that were differentially expressed in the brain of PbA-infected CBA mice: let7i, miR-27a, and miR-150. In contrast, no miRNA changes were detected in the heart, an organ with no known pathology during acute malaria. To investigate the involvement of let-7i, miR-27a, and miR-150 in CM-resistant mice, we assessed the expression levels in gamma interferon knockout (IFN-γ(-/-)) mice on a C57BL/6 genetic background. The expression of let-7i, miR-27a, and miR-150 was unchanged in both wild-type (WT) and IFN-γ(-/-) mice following infection. Overexpression of these three miRNAs during PbA, but not PbK, infection in WT mice may be critical for the triggering of the neurological syndrome via regulation of their potential downstream targets. Our data suggest that in the CBA mouse at least, miRNA may have a regulatory role in the pathogenesis of severe malaria.read more
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Journal ArticleDOI
Cerebral malaria pathogenesis: revisiting parasite and host contributions
Georges E. Grau,Alister Craig +1 more
TL;DR: The range of factors thought to influence the development of Plasmodium falciparum cerebral malaria in humans are examined and the evidence to support their role is reviewed.
Journal ArticleDOI
MicroRNAs in parasitic diseases: Potential for diagnosis and targeting
TL;DR: The dependence of parasites over the host cellular machinery to accomplish infection and complete their biological cycles, together with the potential manipulation of host's responses through parasite miRNAs, point out that the miRNA machinery is particularly interesting to seek for alternative therapeutic approaches against parasites.
Journal ArticleDOI
miR-146a and miR-155 Delineate a MicroRNA Fingerprint Associated with Toxoplasma Persistence in the Host Brain
Dominique Cannella,Dominique Cannella,Marie-Pierre Brenier-Pinchart,Marie-Pierre Brenier-Pinchart,Laurence Braun,Laurence Braun,Jason M. van Rooyen,Jason M. van Rooyen,Alexandre Bougdour,Alexandre Bougdour,Olivier Bastien,Michael S. Behnke,Rose-Laurence Curt,Rose-Laurence Curt,Aurélie Curt,Aurélie Curt,Jeroen P. J. Saeij,L. David Sibley,Hervé Pelloux,Hervé Pelloux,Mohamed-Ali Hakimi,Mohamed-Ali Hakimi +21 more
TL;DR: It is found that miR-146a deficiency led to better control of parasite burden in the gut and most likely of early parasite dissemination in the brain tissue, resulting in the long-term survival of mice.
Journal ArticleDOI
MicroRNAs: Biological Regulators in Pathogen-Host Interactions.
TL;DR: The biological aspects of microRNAs are reviewed, focusing on their roles as regulators of gene expression during pathogen–host interactions and their implications in the immune response against Leishmania, Trypanosoma, Toxoplasma, and Plasmodium infectious diseases.
Journal ArticleDOI
Downregulation of plasma miR-451 and miR-16 in Plasmodium vivax infection.
Supat Chamnanchanunt,Chieri Kuroki,Varunee Desakorn,Mari Enomoto,Vipa Thanachartwet,Duangjai Sahassananda,Jetsumon Sattabongkot,Rachaneeporn Jenwithisuk,Suthat Fucharoen,Saovaros Svasti,Tsukuru Umemura,Tsukuru Umemura +11 more
TL;DR: Analysis of plasma miRNAs obtained from malaria patients and normal subjects suggests that plasma miR-451 and miR -16 are relevant biomarkers for malaria infection.
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