Differential regulation of the rainbow trout ( Oncorhynchus mykiss ) MT-A gene by nuclear factor interleukin-6 and activator protein-1
Citations
19 citations
Cites background from "Differential regulation of the rain..."
...The distal region of the rainbow 319 trout mta promoter possess both activator protein 1 (AP1) and a nuclear factor interleukin-6 320 (NF-IL6) elements that have been shown to play a direct role in mta induction in response to 321 paraquat, an herbicide that induces ROS (Kling et al., 2013)....
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...Some pharmaceuticals and personal care products 316 have been shown to affect reactive oxygen species(ROS) production in rainbow trout gonad 317 cell line (RTG-2) (Fernandez et al., 2013) and PAHs are known to induce free radical 318 production (e.g. Wells and Winn 1996; Zhu et al., 2014)....
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...Effects of human 402 pharmaceuticals on cytotoxicity, EROD activity and ROS production in fish hepatocytes....
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...In addition, FIGCS treated with 322 H2O2, that produces ROS, show an increase in mta and b gene expression, as well as other 323 antioxidant genes (Chung et al., 2005)....
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...The antioxidant gene expression is attenuated by the 324 14 zinc chelator N,N,N’,N’-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), suggesting that the 325 response to ROS in FIGCS is in part mediated by an increase in intracellular zinc 326 concentrations (Chung et al., 2005)....
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14 citations
Cites background from "Differential regulation of the rain..."
...MT promoter activity is regulated by six zinc-finger metalresponsive transcription factor (MTF-1) and a nuclear factor I (NF-I) protein [2]....
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13 citations
Cites background from "Differential regulation of the rain..."
...er region is played by the six zinc-finger-metal-responsive transcription factor (MTF-1) and short cis-acting DNA metal response elements (MREs) as transcriptional activators and a nuclear factor I (NF-I) protein as a downregulator of MT expression [11]....
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12 citations
11 citations
References
20 citations
"Differential regulation of the rain..." refers background in this paper
...NF-IL6 sites are also present in the MT enhancer of other teleosts such as the crucian carp [12] suggesting a conserved function of MT in response to inflammation where NF-IL6 may mediate ROS inducibility....
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...While free radical regulation of mammalian MT genes seem to be mediated via USF/ARE and MRE cis-acting elements [20], teleost MT genes may be regulated via conserved clusters of cis-acting elements sharing high homology to the NF-IL6 and the AP1 consensus core sequences [7,11,12]....
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...Sequencing of several teleost MT enhancer regions have revealed the presence of distally located nuclear factor interleukin 6 (NF-IL6) and AP1 elements, inferring that these elements are involved in a conserved function [7,11-13]....
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19 citations
"Differential regulation of the rain..." refers background in this paper
...However, there have been conflicting reports on the involvement of AP1 in the free radical regulation of MT in teleosts [30,31]....
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...While a deletion construct containing MRE and AP1 responded to ROS in common carp [30], the zebrafish MT gene promoter that contains both AP1 and MRE elements did not respond to ROS [30,31]....
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...To further explore the understanding of MT regulation in teleosts by ROS and tumor promoting agents we have analyzed the relative contribution of AP1 and NF-IL6 elements in the rtMT-A enhancer region....
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...In studies of teleosts there is no clear correlation between the presence of AP1 elements and free radical responsiveness of the MT genes [30,31]....
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...ROS production is highly correlated to pathological responses both at the cellular and organismal level [1]....
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18 citations
"Differential regulation of the rain..." refers background in this paper
...The composite transcription factor AP1 was first identified as a factor mediating optimal basal level expression of the human MT2A (hMT2A) enhancer region [15-17]....
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12 citations
"Differential regulation of the rain..." refers background in this paper
...While free radical regulation of mammalian MT genes seem to be mediated via USF/ARE and MRE cis-acting elements [20], teleost MT genes may be regulated via conserved clusters of cis-acting elements sharing high homology to the NF-IL6 and the AP1 consensus core sequences [7,11,12]....
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...Sequencing of several teleost MT enhancer regions have revealed the presence of distally located nuclear factor interleukin 6 (NF-IL6) and AP1 elements, inferring that these elements are involved in a conserved function [7,11-13]....
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11 citations
"Differential regulation of the rain..." refers background in this paper
...It has been shown that there are more AP1 genes in Fugu than in mammals and that they share high homology in the DNA binding and dimerisation domains [19]....
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