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Journal ArticleDOI

Differential response of cultivated rice to pathogen challenge and abiotic stresses with reference to cationic peroxidase

TL;DR: The peroxidase activity of the seedlings was found to be increased under salt and drought stress conditions and the activity decreased under biotic stress, which would have been the mechanism of overcoming the intrinsic defence in rice by these pathogens.
Abstract: Seedlings of cultivated rice variety ADT43 was investigated after challenging with two different abiotic (drought and salinity) and biotic (sheath blight and bacterial leaf blight pathogens) stresses. Salinity and drought stress reduced the growth of seedlings, mainly the higher conditions (100 mM NaCl and 10 days of drought, respectively). Increased level of MDA content was observed in biotic and abiotic-stress treated seedlings. The highest H2O2 content was observed under salinity-stressed seedlings and lower level observed under biotic stress. Superoxide dismutase activity showed a gradual decrease in all stress conditions compared to control. Salinity stress resulted in highest activity of catalase compared to biotic stress. The peroxidase activity of the seedlings was found to be increased under salt and drought stress conditions and the activity decreased under biotic stress. Drought stress resulted in induced expression of POC1 gene whereas the biotic stress showed lower expression level. Suppressi...
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Journal ArticleDOI
TL;DR: Using two-month-old greenhouse-grown seedlings of the mangrove tree Avicennia officinalis subjected to NaCl treatment, gene expression changes in the roots are profiled by RNA-sequencing to imply that ABA-independent signaling pathways also play a major role in salt tolerance of A. officinalsis.
Abstract: Salinity affects growth and development of plants, but mangroves exhibit exceptional salt tolerance. With direct exposure to salinity, mangrove roots possess specific adaptations to tolerate salt stress. Therefore, studying the early effects of salt on mangrove roots can help us better understand the tolerance mechanisms. Using two-month-old greenhouse-grown seedlings of the mangrove tree Avicennia officinalis subjected to NaCl treatment, we profiled gene expression changes in the roots by RNA-sequencing. Of the 6547 genes that were differentially regulated in response to salt treatment, 1404 and 5213 genes were significantly up- and down-regulated, respectively. By comparative genomics, 93 key salt tolerance-related genes were identified of which 47 were up-regulated. Upon placing all the differentially expressed genes (DEG) in known signaling pathways, it was evident that most of the DEGs involved in ethylene and auxin signaling were up-regulated while those involved in ABA signaling were down-regulated. These results imply that ABA-independent signaling pathways also play a major role in salt tolerance of A. officinalis. Further, ethylene response factors (ERFs) were abundantly expressed upon salt treatment and the Arabidopsis mutant aterf115, a homolog of AoERF114 is characterized. Overall, our results would help in understanding the possible molecular mechanism underlying salt tolerance in plants.

57 citations

Journal ArticleDOI
26 Apr 2019-Agronomy
TL;DR: In this paper, the influence of four waterlogging periods on stomatal conductance (gs), leaf water potential (Ψwf), plant growth, leaf photosynthetic pigments, malondialdehyde (MDA) production, proline content and chlorophyll fluorescence parameters in cape gooseberry plants infected with Fusarium oxysporum f. sp.
Abstract: Cape gooseberry has coped with abiotic and biotic stresses such as prolonged waterlogging periods and vascular wilt in recent years. The aim of this study was to evaluate the influence of four waterlogging periods on stomatal conductance (gs), leaf water potential (Ψwf), plant growth, leaf photosynthetic pigments, malondialdehyde (MDA) production, proline content and chlorophyll fluorescence parameters in cape gooseberry plants infected with Fusarium oxysporum f. sp. physali (Foph). Two-month-old ecotype “Colombia” plants were arranged in a completely randomized factorial design in eight treatments: plants without waterlogging (control), plants with waterlogging for 4, 6 and 8 d with and without Foph, respectively. The area under the disease progress curve was higher in inoculated plants subjected to 6 and 8 d of waterlogging (55.25 and 64.25) compared to inoculated plants but without waterlogging (45.25). The results also showed a lower plant growth, gs, Ψwf, leaf photosynthetic pigments and chlorophyll fluorescence parameters (Fv/Fm, electron transport rate (ETR), Y (II) and qP) as waterlogging periods in plants with Foph increased. However, this group of plants showed a greater proline and malondialdehyde (MDA) accumulation and a higher NPQ. In conclusion, cape gooseberry shows a low acclimation to waterlogging conditions of more than 6 d in soils with Foph.

35 citations

Journal ArticleDOI
18 Jul 2019-PLOS ONE
TL;DR: The potential role of the StEXP genes in potato growth and stress tolerance is shown, and fundamental resources for the future studies in potato breeding are provided.
Abstract: Expansin is a type of cell wall elongation and stress relaxation protein involved in various developmental processes and stress resistances in plant. In this study, we identified 36 potato (Solanum tuberosum L.) genes belonging to the expansin (StEXP) gene family from the genome reference. These genes included 24 α-expansins (StEXPAs), five β-expansins (StEXPBs), one expansin-like A (StEXLA) and six expansin-like B (StEXLBs). The RNA-Seq analysis conducted from a variety of tissue types showed 34 expansins differentially expressed among tissues, some of which only expressed in specific tissues. Most of the StEXPAs and StEXPB2 transcripts were more abundant in young tuber compared with other tissues, suggesting they likely play a role in tuber development. There were 31 genes, especially StEXLB6, showed differential expression under the treatments of ABA, IAA and GA3, as well as under the drought and heat stresses, indicating they were likely involved in potato stress resistance. In addition, the gene co-expression analysis indicated the StEXLBs likely contribute to a wider range of stress resistances compared with other genes. We found the StEXLA and six StEXLBs expressed differently under a range of abiotic stresses (salt, alkaline, heavy metals, drought, heat, and cold stresses), which likely participated in the associated signaling pathways. Comparing with the control group, potato growing under the drought or heat stresses exhibited up-regulation of the all six StEXLB genes in leaves, whereas, the StEXLB3, StEXLB4, StEXLB5 and StEXLB6 showed relatively higher expression levels in roots. This suggested these genes likely played a role in the drought and heat tolerance. Overall, this study has shown the potential role of the StEXP genes in potato growth and stress tolerance, and provided fundamental resources for the future studies in potato breeding.

31 citations

References
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Journal ArticleDOI
TL;DR: It is concluded that isolated chloroplasts upon illumination can undergo a cyclic peroxidation initiated by the light absorbed by chlorophyll.

8,615 citations


"Differential response of cultivated..." refers methods in this paper

  • ...The level of lipid peroxidation was determined by measuring the amount of MDA produced by the thiobarbituric acid reaction according to the method of Heath and Packer (1968)....

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Journal ArticleDOI
TL;DR: Factors which possibly affect the effectiveness of antioxidant protection under oxygen deprivation as well as under other environmental stresses are presented.

3,562 citations


"Differential response of cultivated..." refers background in this paper

  • ...…dismutase (SOD), catalase (CAT) and peroxidases (POX) among peroxidases ascorbate peroxidases and guaiacol peroxidases and glutathione reductase (Blokhina et al. 2003) and non-enzymatic antioxidants includes lipid soluble, membrane-associated tocopherols, water-soluble reductants, ascorbic acid…...

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Journal ArticleDOI
18 Nov 1994-Cell
TL;DR: It is reported here that H2O2 from this oxidative burst not only drives the cross-linking of cell wall structural proteins, but also functions as a local trigger of programmed death in challenged cells and as a diffusible signal for the induction in adjacent cells of genes encoding cellular protectants.

2,740 citations


"Differential response of cultivated..." refers background in this paper

  • ...H2O2 accumulation has been reported to function as an intracellular signal (Levine et al. 1994) stimulating CAT, peroxidase and alternative oxidase....

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Journal ArticleDOI
TL;DR: Identification of ABA metabolic genes has revealed that multiple metabolic steps are differentially regulated to fine-tune the ABA level at both transcriptional and post-transcriptional levels.
Abstract: The level of abscisic acid (ABAabscisic acid) in any particular tissue in a plant is determined by the rate of biosynthesis and catabolism of the hormone. Therefore, identifying all the genes involved in the metabolism is essential for a complete understanding of how this hormone directs plant growth and development. To date, almost all the biosynthetic genes have been identified through the isolation of auxotrophic mutants. On the other hand, among several ABA catabolic pathways, current genomic approaches revealed that Arabidopsis CYP707A genes encode ABA 8′-hydroxylases, which catalyze the first committed step in the predominant ABA catabolic pathway. Identification of ABA metabolic genes has revealed that multiple metabolic steps are differentially regulated to fine-tune the ABA level at both transcriptional and post-transcriptional levels. Furthermore, recent ongoing studies have given new insights into the regulation and site of ABA metabolism in relation to its physiological roles.

1,890 citations


"Differential response of cultivated..." refers background in this paper

  • ...Some genes are induced by variety of stresses and the genes induced by different stresses may overlap (Nambara & Marion-Poll 2005)....

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Journal ArticleDOI
TL;DR: The evidence supports at least a partial antioxidant role in vivo for many classes of plant metabolite, and many other compounds as potential antioxidants can be inferred by their similarity to synthetic antioxidants of related structure.

1,809 citations


"Differential response of cultivated..." refers background in this paper

  • ...…(CAT) and peroxidases (POX) among peroxidases ascorbate peroxidases and guaiacol peroxidases and glutathione reductase (Blokhina et al. 2003) and non-enzymatic antioxidants includes lipid soluble, membrane-associated tocopherols, water-soluble reductants, ascorbic acid and glutathione (Foyer 1993)....

    [...]