Differentiation of Closely Related Carnobacterium Food Isolates Based on 16S-23S Ribosomal DNA Intergenic Spacer Region Polymorphism
Citations
297 citations
Cites background from "Differentiation of Closely Related ..."
...…knowledge on the genes and DNA sequences of Carnobacterium is comparatively sparse, concerning mostly bacteriocin-related genes in the species C. divergens and C. maltaromaticum (Table 2) and 16S rRNA and 16S–23S rRNA gene intergenic spacer sequences (Kabadjova et al., 2002; Rachman et al., 2004)....
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250 citations
Cites methods from "Differentiation of Closely Related ..."
...One such method is ribosomal intergenic spacer analysis (RISA), which involves restriction fragment length analysis of the ribosomal intergenic spacer region (ISR) of bacterial genomes (Kabadjova et al., 2002)....
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208 citations
143 citations
Cites methods or result from "Differentiation of Closely Related ..."
...[32] although the band sizes differed in the two studies....
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...[32] and confirmed the ability of this method to identify isolates of Carnobacterium at the species level (Table 5)....
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...1), ribotyping [43], PCR assays using 16S rDNA-directed primers [2,44] or by ISR analysis (this study, [10,32,65])....
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108 citations
Cites background or methods from "Differentiation of Closely Related ..."
...Partial identification of the isolates was based on cell morphology and lactic acid configuration using the Microzym® system (Biosentec, Toulouse, France) after 48 h of growth in Elliker broth at 15°C and 16S-23S intergenic region profile as described by Kabadjova et al. (2002)....
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...According to Kabadjova et al. (2002) one single amplification product is obtained with Leuconostoc, Lactococcus or Streptococcus thermophilus strains, whereas Lactobacillus or Carnobacterium strains usually give 2 or 3 amplification products....
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...The presence of an inhibition zone was checked visually....
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...The maximum growth rate was calculated by a linear regression method according to the equation: Ln(Ni) = k – µmax*DTi With Ni : initial number of cell in the well and DTi : absorbance detection time in well in midexponential phase....
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References
70,111 citations
"Differentiation of Closely Related ..." refers methods in this paper
...alignment, and the BLAST 2 program (36) was used to represent ISR similarities...
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63,427 citations
"Differentiation of Closely Related ..." refers methods in this paper
...The computer program CLUSTALW (38) was used for sequence...
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...The computer program CLUSTALW (38) was used for sequence alignment, and the BLAST 2 program (36) was used to represent ISR similarities for sequences which did not include 16S or 23S rDNA....
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62,728 citations
"Differentiation of Closely Related ..." refers methods in this paper
...were determined by the dideoxynucleotide chain termination method (35) with...
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987 citations
"Differentiation of Closely Related ..." refers background or methods in this paper
...Thus, the 16S-23S ISR is an important tool for developing DNA-based typing methods because it varies in length and sequence from one species to another (11, 31, 37, 40)....
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...Hence, ISR sequences have been proposed as a useful supplement when 16S rDNA shows insufficient diversity to differentiate recently diverged species (11)....
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381 citations