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Journal ArticleDOI

Digital holographic microscopy for live cell applications and technical inspection

Björn Kemper, +1 more
- 01 Feb 2008 - 
- Vol. 47, Iss: 4
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TLDR
Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells.
Abstract
Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells. The digital holographic feature of (subsequent) numerical focus adjustment makes possible applications for multifocus imaging. An overview of digital holographic microscopy methods is described. Applications of digital holographic microscopy are demonstrated by results obtained from livings cells and engineered surfaces.

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Citations
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Journal ArticleDOI

Nanomedicine--challenge and perspectives.

TL;DR: This Review gives an overview of selected recent developments and applications of nanomedicine.
Journal ArticleDOI

Principles and techniques of digital holographic microscopy

TL;DR: Digital holography is an emerging field of new paradigm in general imaging applications as discussed by the authors, and a review of a subset of the research and development activities in digital holographic microscopy techniques and applications is presented.
Journal ArticleDOI

Quantitative phase imaging techniques for the study of cell pathophysiology: from principles to applications.

TL;DR: The principles of QPI are presented and some of the recent applications ranging from cell homeostasis to infectious diseases and cancer are highlighted, to provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology.
Journal ArticleDOI

High-resolution transport-of-intensity quantitative phase microscopy with annular illumination

TL;DR: In this article, a matched annular illumination was proposed to boost the phase contrast for low spatial frequencies, and significantly improved the practical imaging resolution to near the incoherent diffraction limit, achieving a transverse resolution up to 208 nm.
Journal ArticleDOI

Transport of intensity equation: a tutorial

TL;DR: A new era in which strict coherence and interferometry are no longer prerequisites for quantitative phase imaging and diffraction tomography is highlighted, paving the way toward new generation label-free three-dimensional microscopy, with applications in all branches of biomedicine.
References
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Journal ArticleDOI

A new microscopic principle.

Dennis Gabor
- 01 May 1948 - 
TL;DR: An improvement of the resolution by one decimal wotild require a correction of the objective to four decimals, a practically hopeless task.
Journal ArticleDOI

Stimulated Optical Radiation in Ruby

TL;DR: Schawlow and Townes as discussed by the authors proposed a technique for the generation of very monochromatic radiation in the infra-red optical region of the spectrum using an alkali vapour as the active medium.
Journal Article

Stimulated optical radiation in ruby

T. H. Maiman
TL;DR: Schawlow and Townes as discussed by the authors proposed a technique for the generation of very monochromatic radiation in the infra-red optical region of the spectrum using an alkali vapour as the active medium.
Journal ArticleDOI

Phase-shifting digital holography

TL;DR: A new method is proposed in which the distribution of complex amplitude at a plane is measured by phase-shifting interferometry and then Fresnel transformed by a digital computer, which can reconstruct an arbitrary cross section of a three-dimensional object with higher image quality and a wider viewing angle than from conventional digital holography using an off-axis configuration.
Journal ArticleDOI

Reconstructed Wavefronts and Communication Theory

TL;DR: In this paper, a two-step imaging process is described from a communication-theory viewpoint, which consists of three well-known operations: a modulation, a frequency dispersion, and a square-law detection.
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