Direct Lineage Conversions: Unnatural but useful?
Thomas Vierbuchen,Marius Wernig +1 more
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TLDR
Direct lineage conversion could provide important new sources of human cells for modeling disease processes or for cellular-replacement therapies and develop methods for robustly and efficiently generating human cell types of interest.Abstract:
Classic experiments such as somatic cell nuclear transfer into oocytes and cell fusion demonstrated that differentiated cells are not irreversibly committed to their fate. More recent work has built on these conclusions and discovered defined factors that directly induce one specific cell type from another, which may be as distantly related as cells from different germ layers. This suggests the possibility that any specific cell type may be directly converted into any other if the appropriate reprogramming factors are known. Direct lineage conversion could provide important new sources of human cells for modeling disease processes or for cellular-replacement therapies. For future applications, it will be critical to carefully determine the fidelity of reprogramming and to develop methods for robustly and efficiently generating human cell types of interest.read more
Citations
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Reconstructing and Reprogramming the Tumor-Propagating Potential of Glioblastoma Stem-like Cells
Mario L. Suvà,Mario L. Suvà,Mario L. Suvà,Esther Rheinbay,Esther Rheinbay,Esther Rheinbay,Shawn M. Gillespie,Shawn M. Gillespie,Shawn M. Gillespie,Anoop P. Patel,Anoop P. Patel,Hiroaki Wakimoto,Samuel D. Rabkin,Nicolo Riggi,Nicolo Riggi,Anthony Wei Shine Chi,Daniel P. Cahill,Brian V. Nahed,William T. Curry,Robert L. Martuza,Miguel Rivera,Miguel Rivera,Nikki E. Rossetti,Nikki E. Rossetti,Simon Kasif,Samantha Beik,Sabah Kadri,Itay Tirosh,Ivo Wortman,Alex K. Shalek,Orit Rozenblatt-Rosen,Aviv Regev,Aviv Regev,Aviv Regev,David N. Louis,Bradley E. Bernstein,Bradley E. Bernstein,Bradley E. Bernstein +37 more
TL;DR: In this paper, a core set of neurodevelopmental transcription factors (POU3F2, SOX2, SALL2, and OLIG2) are identified as essential for GBM propagation.
Journal ArticleDOI
Epigenetic Reprogramming in Cancer
Mario L. Suvà,Nicolo Riggi,Nicolo Riggi,Nicolo Riggi,Bradley E. Bernstein,Bradley E. Bernstein,Bradley E. Bernstein +6 more
TL;DR: In this article, the authors review conceptual parallels between the respective biological phenomena, highlighting important interrelationships among transcription factors, chromatin regulators, and preexisting epigenetic states, and provide insights into oncogenic transformation, tumor heterogeneity, and cancer stem cell models.
Reconstructing and Reprogramming the Tumor-Propagating Potential of Glioblastoma Stem-like Cells
Mario L. Suvà,Esther Rheinbay,Shawn M. Gillespie,Anoop P. Patel,Hiroaki Wakimoto,Samuel D. Rabkin,Nicolo Riggi,Andrew S. Chi,Daniel P. Cahill,Brian V. Nahed,William T. Curry,Robert L. Martuza,Miguel Rivera,Nikki E. Rossetti,Simon Kasif,Samantha Beik,Sabah Kadri,Itay Tirosh,Ivo Wortman,Alex K. Shalek,Orit Rozenblatt-Rosen,David N. Louis,Bradley E. Bernstein,Aviv Regev +23 more
TL;DR: This study identifies a core set of neurodevelopmental TFs (POU3F2, SOX2, SALL2, and OLIG2) essential for GBM propagation and reconstructs a network model that highlights critical interactions and identifies candidate therapeutic targets for eliminating TPCs.
Journal ArticleDOI
Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons.
Orly L. Wapinski,Thomas Vierbuchen,Kun Qu,Qian Yi Lee,Soham Chanda,Daniel R. Fuentes,Paul G. Giresi,Yi Han Ng,Samuele Marro,Norma F. Neff,Daniela Drechsel,Ben Martynoga,Diogo S. Castro,Ashley E. Webb,Thomas C. Südhof,Anne Brunet,François Guillemot,Howard Y. Chang,Howard Y. Chang,Marius Wernig +19 more
TL;DR: A precise match between pioneer factors and the chromatin context at key target genes is determinative for transdifferentiation to neurons and likely other cell types.
Journal ArticleDOI
Reprogramming of human fibroblasts toward a cardiac fate
Young-Jae Nam,Kunhua Song,Xiang Luo,Edward Daniel,Kaleb Lambeth,Katherine West,Joseph A. Hill,J. Michael DiMaio,Linda A. Baker,Rhonda Bassel-Duby,Eric N. Olson +10 more
TL;DR: Findings indicate that human fibroblasts can be reprogrammed to cardiac-like myocytes by forced expression of cardiac transcription factors with muscle-specific microRNAs and represent a step toward possible therapeutic application of this reprogramming approach.
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