Journal ArticleDOI
Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads
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TLDR
Deviations from the force curves predicted by the freely jointed chain model suggest that DNA has significant local curvature in solution, and the effect of bend-inducing cis-diamminedichloroplatinum (II) was large and supports the hypothesis of natural curvatures in DNA.Abstract:
Single DNA molecules were chemically attached by one end to a glass surface and by their other end to a magnetic bead. Equilibrium positions of the beads were observed in an optical microscope while the beads were acted on by known magnetic and hydrodynamic forces. Extension versus force curves were obtained for individual DNA molecules at three different salt concentrations with forces between 10(-14) and 10(-11) newtons. Deviations from the force curves predicted by the freely jointed chain model suggest that DNA has significant local curvature in solution. Ethidium bromide and 4',6-diamidino-2-phenylindole had little effect on the elastic response of the molecules, but their extent of intercalation was directly measured. Conversely, the effect of bend-inducing cis-diamminedichloroplatinum (II) was large and supports the hypothesis of natural curvature in DNA.read more
Citations
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Microfluidics: Fluid physics at the nanoliter scale
Todd M. Squires,Stephen R. Quake +1 more
TL;DR: A review of the physics of small volumes (nanoliters) of fluids is presented, as parametrized by a series of dimensionless numbers expressing the relative importance of various physical phenomena as mentioned in this paper.
Journal ArticleDOI
Overstretching B-DNA: The Elastic Response of Individual Double-Stranded and Single-Stranded DNA Molecules
TL;DR: DsDNA molecules in aqueous buffer undergo a highly cooperative transition into a stable form with 5.8 angstroms rise per base pair, that is, 70% longer than B-form dsDNA, which may play a significant role in the energetics of DNA recombination.
Journal ArticleDOI
DNA in a material world
TL;DR: The specific bonding of DNA base pairs provides the chemical foundation for genetics and this powerful molecular recognition system can be used in nanotechnology to direct the assembly of highly structured materials with specific nanoscale features, as well as in DNA computation to process complex information.
Journal ArticleDOI
Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy
Keir C. Neuman,Attila Nagy +1 more
TL;DR: These techniques are described and illustrated with examples highlighting current capabilities and limitations of single-molecule force spectroscopy.
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A DNA-fuelled molecular machine made of DNA
Bernard Yurke,Andrew J. Turberfield,Andrew J. Turberfield,Allen P. Mills,Friedrich C. Simmel,Jennifer L. Neumann +5 more
TL;DR: The construction of a DNA machine in which the DNA is used not only as a structural material, but also as ‘fuel’; each cycle produces a duplex DNA waste product.
References
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Book
Principles of Nucleic Acid Structure
TL;DR: The goal of this series is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields.
Journal ArticleDOI
Flexibility of DNA
TL;DR: To most carefully address the influence of such factors as base sequence, DNA damage, and drug or protein interaction on the flexibility of DNA, methods are required that are most sensitive for DNA molecules of less than 500-1000 bp.
Journal ArticleDOI
Curved DNA without A-A: experimental estimation of all 16 DNA wedge angles.
TL;DR: This work has applied the wedge model of DNA curvature to a large body of experimental data and found that the stacks AG/CT, CG/CG, GA/TC, and GC/GC, in addition to AA/TT, have large wedge values.