Distinctive nuclear organisation of centromeres and regions involved in pluripotency in human embryonic stem cells
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14 citations
Cites background from "Distinctive nuclear organisation of..."
...Z to development and tumorigenesis through nuclear organization Previous studies have shown that the intranuclear repositioning of chromosomal loci is linked to the differentiation of normal and tumorigenic cells (Cremer et al., 2003; Meaburn et al., 2009; Wiblin et al., 2005; Wiech et al., 2005)....
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...linked to the differentiation of normal and tumorigenic cells (Cremer et al., 2003; Meaburn et al., 2009; Wiblin et al., 2005; Wiech et al., 2005)....
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14 citations
Cites background from "Distinctive nuclear organisation of..."
...…the lack of reference points in the nucleus, many studies have focused on the radial distribution of nuclear structures, which are computed by measuring distance to either the nuclear border (Wiblin et al., 2005) or a computed nucleus center that has no biological meaning (Weierich et al., 2003)....
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...…with the nuclear lamina, as well as with the nucleolus, in both mouse and human cells and that their distribution in the interphase nucleus is modified in relation to the cell cycle, as well as to physiological and differentiation states (reviewed in Pluta et al., 1995; Wiblin et al., 2005)....
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...Owing to the lack of reference points in the nucleus, many studies have focused on the radial distribution of nuclear structures, which are computed by measuring distance to either the nuclear border (Wiblin et al., 2005) or a computed nucleus center that has no biological meaning (Weierich et al....
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...It will be interesting to check whether the previously reported increased association of CRs with the nuclear border observed during cell differentiation reflects global reorganization of heterochromatin in these cells or is just the consequence of a higher proportion of nonproliferating cells (Wiblin et al., 2005)....
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...Since the pioneering work of Manuelidis and coworkers (Manuelidis, 1984, 1985), several groups have shown that centromeric regions are mobile structures that associate with the nuclear lamina, as well as with the nucleolus, in both mouse and human cells and that their distribution in the interphase nucleus is modified in relation to the cell cycle, as well as to physiological and differentiation states (reviewed in Pluta et al., 1995; Wiblin et al., 2005)....
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14 citations
13 citations
Cites background from "Distinctive nuclear organisation of..."
...For example, the stem cell specific genes Nanog and Oct4 acquire differential positioning in the nucleus as their expression levels change during differentiation of human embryonic stem cells [9]....
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12 citations
References
15,555 citations
"Distinctive nuclear organisation of..." refers background or methods in this paper
...Human ES cells have been derived from the inner cell mass of blastocysts, and as well as being able to self-renew, they have the ability to differentiate into all three embryonic germ layers when injected into severe combined immunodeficient mice (Thomson et al., 1998)....
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...Human ES cell culture and analysis Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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...Germ cells and stem cells in contrast have active telomerase, and robust telomerase activity is detected in hES cells (Thomson et al., 1998)....
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...Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al....
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2,092 citations
"Distinctive nuclear organisation of..." refers methods in this paper
...Human ES cell culture and analysis Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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..., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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1,776 citations
"Distinctive nuclear organisation of..." refers background in this paper
..., 2002b) and hES cells (Ramalho-Santos et al., 2002), but which is located in a low gene-density region at 11p13 (32Mb), remains inside the CT (Table 1)....
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...In contrast, RCN, which is expressed in both LCLs (Mahy et al., 2002b) and hES cells (Ramalho-Santos et al., 2002), but which is located in a low gene-density region at 11p13 (32Mb), remains inside the CT (Table 1)....
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1,046 citations
"Distinctive nuclear organisation of..." refers background in this paper
...It is interesting to note that recurrent gains of chromosome 12, including iso12p, have been found in human ES cells (Draper et al., 2004)....
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914 citations
"Distinctive nuclear organisation of..." refers background or methods in this paper
...HSA18 is found towards the nuclear periphery in a variety of differentiated cells and HSA19 is in the centre of the nucleus (Croft et al., 1999; Cremer et al., 2003)....
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...Hybridisation was as described previously (Croft et al., 1999) but with the denaturing time reduced to 1....
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...Chromosome paints were labelled with biotin-16-dUTP by nick translation or by PCR amplification (Croft et al., 1999) or obtained commercially (Cambio)....
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...Slides were then subjected to freeze-thaw in 20% glycerol/PBS and FISH was carried out as described previously (Croft et al., 1999)....
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...The radial distribution of CTs was determined in 2D specimens by an erosion script, as previously described (Croft et al., 1999)....
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