Distinctive nuclear organisation of centromeres and regions involved in pluripotency in human embryonic stem cells
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...This conclusion is further supported by the observations that the stem cell-specific genes Nanog and Oct4 acquire differential positioning as their expression levels change during differentiation of human embryonic stem cells (Wiblin et al. 2005)....
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12 citations
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...In addition, repositioning of chromosomal arms has been observed in the case of NANOG, which is highly expressed during pluripotency, where the short arm of chromosome 12 (12p) is found closer to the center of the nucleus in human ESCs than in differentiated cells (Wiblin et al. 2005)....
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...Notably, visible chromocenters are absent in human cells, but staining for centromere protein C (CENPC) reveals that centromeres in human ESCs localize further away from the nuclear periphery than most other cell types (Wiblin et al. 2005)....
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11 citations
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...In many experiments, the active genes were positioned in the nuclear interior (Zink et al. 2004, Wiblin et al. 2005, Harničarová et al. 2006, Williams et al. 2006) or at the chromosome periphery (Kurz et al. 1996, Dietzel et al. 1999, Harničarová et al. 2006), as well as on the chromatin loops…...
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...This does not concern some most actively transcribed genes, which loop out of the chromosome territories and appear to be located near the so-called PML compartments [23, 24]....
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References
15,555 citations
"Distinctive nuclear organisation of..." refers background or methods in this paper
...Human ES cells have been derived from the inner cell mass of blastocysts, and as well as being able to self-renew, they have the ability to differentiate into all three embryonic germ layers when injected into severe combined immunodeficient mice (Thomson et al., 1998)....
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...Human ES cell culture and analysis Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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...Germ cells and stem cells in contrast have active telomerase, and robust telomerase activity is detected in hES cells (Thomson et al., 1998)....
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...Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al....
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2,092 citations
"Distinctive nuclear organisation of..." refers methods in this paper
...Human ES cell culture and analysis Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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..., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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1,776 citations
"Distinctive nuclear organisation of..." refers background in this paper
..., 2002b) and hES cells (Ramalho-Santos et al., 2002), but which is located in a low gene-density region at 11p13 (32Mb), remains inside the CT (Table 1)....
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...In contrast, RCN, which is expressed in both LCLs (Mahy et al., 2002b) and hES cells (Ramalho-Santos et al., 2002), but which is located in a low gene-density region at 11p13 (32Mb), remains inside the CT (Table 1)....
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1,046 citations
"Distinctive nuclear organisation of..." refers background in this paper
...It is interesting to note that recurrent gains of chromosome 12, including iso12p, have been found in human ES cells (Draper et al., 2004)....
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914 citations
"Distinctive nuclear organisation of..." refers background or methods in this paper
...HSA18 is found towards the nuclear periphery in a variety of differentiated cells and HSA19 is in the centre of the nucleus (Croft et al., 1999; Cremer et al., 2003)....
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...Hybridisation was as described previously (Croft et al., 1999) but with the denaturing time reduced to 1....
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...Chromosome paints were labelled with biotin-16-dUTP by nick translation or by PCR amplification (Croft et al., 1999) or obtained commercially (Cambio)....
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...Slides were then subjected to freeze-thaw in 20% glycerol/PBS and FISH was carried out as described previously (Croft et al., 1999)....
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...The radial distribution of CTs was determined in 2D specimens by an erosion script, as previously described (Croft et al., 1999)....
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