Distinctive nuclear organisation of centromeres and regions involved in pluripotency in human embryonic stem cells
Citations
82 citations
Cites background from "Distinctive nuclear organisation of..."
...…cues, signaling pathways, transcription factors, reactive oxygen species (ROS), extracellular matrix (ECM), microRNAs, and chromosome structural modifiers ( Sinha et al., 2004; Qiu et al., 2005; Wiblin et al., 2005; Rana, 2007; Xiao et al., 2009; Kurpinski et al., 2010; Suzuki et al., 2010)....
[...]
81 citations
Cites background from "Distinctive nuclear organisation of..."
...These observations are anecdotal, however, and exceptions also exist: for example, the OCT4 pluripotency gene, in contrast to NANOG, does not show changes in radial positioning upon differentiation [25,55]....
[...]
...This may reflect the fact that OCT4 is situated much further from the edge of its chromosomal territory in LCLs than NANOG [55]....
[...]
...From the evidence available it seems that most chromosomes do not change their radial positioning during differentiation [27,55]....
[...]
...For example, centromeres are found more frequently associated with the nuclear rim or the nucleolus after differentiation [27,55]....
[...]
77 citations
76 citations
76 citations
References
15,555 citations
"Distinctive nuclear organisation of..." refers background or methods in this paper
...Human ES cells have been derived from the inner cell mass of blastocysts, and as well as being able to self-renew, they have the ability to differentiate into all three embryonic germ layers when injected into severe combined immunodeficient mice (Thomson et al., 1998)....
[...]
...Human ES cell culture and analysis Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
[...]
...Germ cells and stem cells in contrast have active telomerase, and robust telomerase activity is detected in hES cells (Thomson et al., 1998)....
[...]
...Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al....
[...]
2,092 citations
"Distinctive nuclear organisation of..." refers methods in this paper
...Human ES cell culture and analysis Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
[...]
..., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
[...]
1,776 citations
"Distinctive nuclear organisation of..." refers background in this paper
..., 2002b) and hES cells (Ramalho-Santos et al., 2002), but which is located in a low gene-density region at 11p13 (32Mb), remains inside the CT (Table 1)....
[...]
...In contrast, RCN, which is expressed in both LCLs (Mahy et al., 2002b) and hES cells (Ramalho-Santos et al., 2002), but which is located in a low gene-density region at 11p13 (32Mb), remains inside the CT (Table 1)....
[...]
1,046 citations
"Distinctive nuclear organisation of..." refers background in this paper
...It is interesting to note that recurrent gains of chromosome 12, including iso12p, have been found in human ES cells (Draper et al., 2004)....
[...]
914 citations
"Distinctive nuclear organisation of..." refers background or methods in this paper
...HSA18 is found towards the nuclear periphery in a variety of differentiated cells and HSA19 is in the centre of the nucleus (Croft et al., 1999; Cremer et al., 2003)....
[...]
...Hybridisation was as described previously (Croft et al., 1999) but with the denaturing time reduced to 1....
[...]
...Chromosome paints were labelled with biotin-16-dUTP by nick translation or by PCR amplification (Croft et al., 1999) or obtained commercially (Cambio)....
[...]
...Slides were then subjected to freeze-thaw in 20% glycerol/PBS and FISH was carried out as described previously (Croft et al., 1999)....
[...]
...The radial distribution of CTs was determined in 2D specimens by an erosion script, as previously described (Croft et al., 1999)....
[...]