Distinctive nuclear organisation of centromeres and regions involved in pluripotency in human embryonic stem cells
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...Differentiation is generally accompanied by increased clustering into fewer but larger chromocenters and their relocation to the nuclear periphery (Beil et al. 2002; Weierich et al. 2003; Mayer et al. 2005; Wiblin et al. 2005)....
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...Instead,microscopy studies have previously shown that chromocenters generally occupy more peripheral territory during lineage commitment (Weierich et al. 2003; Mayer et al. 2005; Wiblin et al. 2005)....
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72 citations
Cites background or result from "Distinctive nuclear organisation of..."
...Although the radial organization of chromosome territories does not appear to change substantially during differentiation of hESCs [Wiblin et al., 2005; Bartova et al., 2008a], greater chromatin factor mobility and genome-wide expression is seen in mouse ESC nuclei, suggesting a less fixed and more…...
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...Of the limited number of studies examining nuclear structure in ES cells [Wiblin et al., 2005; Meshorer and Misteli, 2006], most compare undifferentiated ES cells to more mature somatic nuclei or ES cells after differentiation has progressed....
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...A few observations in human ESC suggest some limited differences from somatic chromosome organization [Wiblin et al., 2005; Bartova et al., 2008b], however, the investigation of nuclear structure in relation to early embryonic programming has barely begun....
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...For example, consistent with previous observations of pluripotent ES cells [Wiblin et al., 2005], we saw no obvious difference in the overall distribution of telomeres (Fig....
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69 citations
69 citations
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...Moreover, in cells with decondensed chromatin, such as pluripotent embryonic stem cells (ESCs), active genes are positioned on chromatin loops extended away from their CTs (Volpi et al. 2000; Mahy et al. 2002, Williams et al. 2002; Wiblin et al. 2005, Bártová et al. 2008c)....
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References
15,555 citations
"Distinctive nuclear organisation of..." refers background or methods in this paper
...Human ES cells have been derived from the inner cell mass of blastocysts, and as well as being able to self-renew, they have the ability to differentiate into all three embryonic germ layers when injected into severe combined immunodeficient mice (Thomson et al., 1998)....
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...Human ES cell culture and analysis Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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...Germ cells and stem cells in contrast have active telomerase, and robust telomerase activity is detected in hES cells (Thomson et al., 1998)....
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...Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al....
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2,092 citations
"Distinctive nuclear organisation of..." refers methods in this paper
...Human ES cell culture and analysis Human ES cell lines H1 (46XY), H7 and H9 (46XX) (Thomson et al., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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..., 1998) were grown as previously described, with minor modification (Xu et al., 2001)....
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1,776 citations
"Distinctive nuclear organisation of..." refers background in this paper
..., 2002b) and hES cells (Ramalho-Santos et al., 2002), but which is located in a low gene-density region at 11p13 (32Mb), remains inside the CT (Table 1)....
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...In contrast, RCN, which is expressed in both LCLs (Mahy et al., 2002b) and hES cells (Ramalho-Santos et al., 2002), but which is located in a low gene-density region at 11p13 (32Mb), remains inside the CT (Table 1)....
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1,046 citations
"Distinctive nuclear organisation of..." refers background in this paper
...It is interesting to note that recurrent gains of chromosome 12, including iso12p, have been found in human ES cells (Draper et al., 2004)....
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914 citations
"Distinctive nuclear organisation of..." refers background or methods in this paper
...HSA18 is found towards the nuclear periphery in a variety of differentiated cells and HSA19 is in the centre of the nucleus (Croft et al., 1999; Cremer et al., 2003)....
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...Hybridisation was as described previously (Croft et al., 1999) but with the denaturing time reduced to 1....
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...Chromosome paints were labelled with biotin-16-dUTP by nick translation or by PCR amplification (Croft et al., 1999) or obtained commercially (Cambio)....
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...Slides were then subjected to freeze-thaw in 20% glycerol/PBS and FISH was carried out as described previously (Croft et al., 1999)....
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...The radial distribution of CTs was determined in 2D specimens by an erosion script, as previously described (Croft et al., 1999)....
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