Journal ArticleDOI
DNA vaccines against human immunodeficiency virus type 1
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TLDR
This review focuses on the stimulation of HIV‐specific T cells and discusses in the context of the current ‘state‐of‐art’ of DNA vaccines, the areas where this technology might assist either alone or as a part of more complex vaccine formulations in the HIV vaccine development.Abstract:
Development of a vaccine against human immunodeficiency virus type 1 (HIV-1) is the main hope for controlling the acquired immunodeficiency syndrome pandemic. An ideal HIV vaccine should induce neutralizing antibodies, CD4+ helper T cells, and CD8+ cytotoxic T cells. While the induction of broadly neutralizing antibodies remains a highly challenging goal, there are a number of technologies capable of inducing potent cell-mediated responses in animal models, which are now starting to be tested in humans. Naked DNA immunization is one of them. This review focuses on the stimulation of HIV-specific T cells and discusses in the context of the current 'state-of-art' of DNA vaccines, the areas where this technology might assist either alone or as a part of more complex vaccine formulations in the HIV vaccine development.read more
Citations
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Journal ArticleDOI
Clinical experience with plasmid DNA- and modified vaccinia virus Ankara-vectored human immunodeficiency virus type 1 clade A vaccine focusing on T-cell induction
TL;DR: These trials demonstrated that the pTHr.HIVA vaccine alone primed consistently weak and mainly CD4(+), but also CD8(+) T-cell responses, and the MVA is a useful boosting vector for human subunit genetic vaccines.
Journal ArticleDOI
Evaluating the Immunogenicity of a Disulfide-Stabilized, Cleaved, Trimeric Form of the Envelope Glycoprotein Complex of Human Immunodeficiency Virus Type 1
Simon Beddows,Norbert Schülke,Marc W. Kirschner,Kelly Barnes,Michael Franti,Elizabeth Michael,Thomas J. Ketas,Rogier W. Sanders,Paul J. Maddon,William C. Olson,John P. Moore +10 more
TL;DR: In this article, the authors report on the antibody responses generated in rabbits against a soluble, cleaved, trimeric form of HIV-1 JR-FL Env. Neutralization of these viruses was immunoglobulin mediated and was predominantly caused by antibodies to gp120 epitopes.
Journal ArticleDOI
Multimeric Soluble CD40 Ligand and GITR Ligand as Adjuvants for Human Immunodeficiency Virus DNA Vaccines
Geoffrey W. Stone,Suzanne Barzee,Victoria Snarsky,Kristin Kee,Kristin Kee,Celsa A. Spina,Celsa A. Spina,Xiao Fang Yu,Richard S. Kornbluth +8 more
TL;DR: Multimeric CD40L and GITR ligand augmented the CD8+ T-cell, CD4-cell and antibody responses to DNA vaccination and are new adjuvants for DNA vaccines.
Journal ArticleDOI
Immunization of rhesus macaques with a polyvalent DNA prime/protein boost human immunodeficiency virus type 1 vaccine elicits protective antibody response against simian human immunodeficiency virus of R5 phenotype
Ranajit Pal,Shixia Wang,Vaniambadi S. Kalyanaraman,B. C. Nair,Stephen Whitney,Timothy Keen,Lindsey Hocker,Lauren Hudacik,Nicolas Rose,Innocent Mboudjeka,Siyuan Shen,Te-Hui Wu-Chou,David C. Montefiori,John R. Mascola,Phillip D. Markham,Shan Lu +15 more
TL;DR: Priming with DNA encoding Env antigens from multiple HIV-1 clades followed by boosting with homologous Env proteins elicits anti-HIV-1 immune responses capable of protecting macaques against mucosal transmission of R5 tropic SHIV isolate.
Journal ArticleDOI
Long-term storage of DNA-free RNA for use in vaccine studies
TL;DR: The addition of trehalose permitted stable storage of functional RNA at 4 degrees C for up to 10 months and this permits the development of RNA vaccines, even in developing countries where only minimum storage conditions can be achieved.
References
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