Does intracellular metabolite diffusion limit post-contractile recovery in burst locomotor muscle?
Citations
566 citations
71 citations
Cites background from "Does intracellular metabolite diffu..."
...…density (Kinsey et al., 2007; Locke and Kinsey, 2008), from respiration rates of isolated mitochondria (Burpee et al., 2010), or from direct measurements of ATP turnover using NMR in isolated muscles or in vivo (Kinsey et al., 2005; Hardy et al., 2006; Nyack et al., 2007; Jimenez et al., 2008)....
[...]
...This allows phosphagen resynthesis to occur faster than our reaction–diffusion model predicted that it should in the large fibers (Kinsey et al., 2005)....
[...]
...THE JOURNAL OF EXPERIMENTAL BIOLOGY 264 can be <0.1mol ATPg–1min–1 (Kinsey et al., 2005; Nyack et al., 2007), whereas in insect flight muscle it can exceed 2000mol ATPg–1min–1 (Suarez, 1998)....
[...]
69 citations
Cites methods from "Does intracellular metabolite diffu..."
...This idea, called the ‘optimal fiber size hypothesis’, was proposed by Johnston et al. (2003, 2004) for notothenioids and Arctic char evolution and by Kinsey et al. (2005) for blue crabs....
[...]
57 citations
Cites background or methods from "Does intracellular metabolite diffu..."
...Coupling experimental data with a reaction–diffusion mathematical model, however, we found that the observed rate of AP resynthesis following burst contraction in the anaerobic locomotor fibers of small and large blue crabs was not dramatically limited by intracellular metabolite diffusion (Kinsey et al., 2005)....
[...]
...…data with a reaction–diffusion mathematical model, however, we found that the observed rate of AP resynthesis following burst contraction in the anaerobic locomotor fibers of small and large blue crabs was not dramatically limited by intracellular metabolite diffusion (Kinsey et al., 2005)....
[...]
...…during fiber growth can be demonstrated by rearranging the equation described above for displacement, , to solve for diffusion time, t, using time-dependent diffusion coefficients and mitochondrial spacing data from a crustacean muscle example (Kinsey and Moerland, 2002; Kinsey et al., 2005)....
[...]
...Quantitative analyses of some skeletal muscle types indicate that the extent to which metabolite diffusion limits aerobic flux is variable (e.g. Mainwood and Rakusan, 1982; Meyer et al., 1984; Tyler and Sidell, 1984; Egginton and Sidell, 1989; Hubley et al., 1997; Kemp et al., 1998; Kinsey et al., 2005; Hardy et al., 2006; Nyack et al., 2007), but a broad analysis that encompasses diffusion effects over the full spectrum of muscle fiber designs is lacking....
[...]
...…variable (e.g. Mainwood and Rakusan, 1982; Meyer et al., 1984; Tyler and Sidell, 1984; Egginton and Sidell, 1989; Hubley et al., 1997; Kemp et al., 1998; Kinsey et al., 2005; Hardy et al., 2006; Nyack et al., 2007), but a broad analysis that encompasses diffusion effects over the full spectrum of…...
[...]
42 citations
Cites background or methods or result from "Does intracellular metabolite diffu..."
...…ADP, Pi, AP/PCr and arginine/creatine) does not lead to sizable intracellular concentration gradients in the very large anaerobic fibers of crustacean or fish muscle, despite the fact that diffusion might occur over hundreds of microns (Kinsey et al., 2005; Hardy et al., 2006; Nyack et al., 2007)....
[...]
...The area under each peak was integrated using Xwin-NMR software to yield the relative concentrations of each metabolite, and these values were converted to concentration by assuming a total HEP concentration of 50mmol l–1, which is characteristic of crustacean white muscle (see Kinsey et al., 2005)....
[...]
...This is consistent with prior suggestions that O2 delivery is a greater constraint on muscle design and function than is HEP diffusion (Kinsey et al., 2005; Hardy et al., 2006; Nyack et al., 2007)....
[...]
...Exceeding this maximum size might therefore compromise aerobic metabolism, which relies on oxygen flux from the blood to the mitochondria and ATP-equivalent diffusion within the cytoplasm (Mainwood and Rakusan, 1982; Meyer, 1988; Hubley et al., 1997; Boyle et al., 2003; Johnson et al., 2004; Kinsey et al., 2005; Hardy et al., 2006)....
[...]
...Presumably, adult crabs are exploiting anaerobic metabolism to accelerate certain key phases of recovery such as AP resynthesis, the rate of which would otherwise be restricted by large fiber size (Kinsey and Moerland, 2002; Boyle et al., 2003; Johnson et al., 2004; Kinsey et al., 2005)....
[...]
References
18 citations
"Does intracellular metabolite diffu..." refers background in this paper
...In addition to the above argument, the partial pressure of oxygen (PO∑) in crustacean blood (including blue crabs) is low relative to that of muscle from active vertebrate species (Gannon and Wheatly, 1995; Forgue et al., 2001)....
[...]
...Forgue et al. (2001) have made complimentary arguments that the low blood PO∑ in crustaceans limits resting metabolic rate to reduce costs during periods of inactivity....
[...]
18 citations
16 citations
"Does intracellular metabolite diffu..." refers background or result in this paper
...Data from small prawn anaerobic tail muscle that would not be expected to have large fibers (Thébault et al., 1987) and from isolated dogfish white muscle, which resynthesizes phosphocreatine (PCr) using only aerobic metabolism and has a mitochondrial fractional area of ~0.01 (Curtin et al., 1997),…...
[...]
...This is consistent with the fact that phosphagen recovery rates in mammalian muscle (e.g. Vicini and Kushmerick, 2000) are >10-fold higher than rates in crustacean muscle (Thébault et al., 1987)....
[...]
13 citations
"Does intracellular metabolite diffu..." refers background in this paper
...…and distant sites of ATP utilization, such as in some muscle fibers with a higher aerobic capacity than examined here (Meyer et al., 1984; Stokes and Josephson, 1992; Vendelin et al., 2000; Saks et al., 2003; Suarez, 2003) or in the flagellum of spermatozoa, which has been the subject of…...
[...]
...This view has been suggested previously to explain mitochondrial clustering at the sarcolemma in non-giant mammalian (Mainwood and Rakusan, 1982) and crustacean muscle (Stokes and Josephson, 1992)....
[...]
...These are likely to include systems with relatively high rates of ATP production/consumption and distant sites of ATP utilization, such as in some muscle fibers with a higher aerobic capacity than examined here (Meyer et al., 1984; Stokes and Josephson, 1992; Vendelin et al., 2000; Saks et al., 2003; Suarez, 2003) or in the flagellum of spermatozoa, which has been the subject of many reaction–diffusion analyses (e....
[...]
13 citations
"Does intracellular metabolite diffu..." refers methods or result in this paper
...However, in resting animals, the AP/Pi ratios in extracts were always similar to previous values observed in intact, superfused crustacean white muscle (Kinsey and Ellington, 1996), and there were no significant differences in the AP/Pi ratios between size classes (data not shown)....
[...]
...The resting metabolite concentrations for crustacean anaerobic locomotor fibers were obtained from a combination of the data in Head and Baldwin (1986), 31P-NMR spectra collected by Kinsey and Ellington (1996) and calculations using the AK equilibrium constant (Teague and Dobson, 1999)....
[...]