Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli
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"Driving Forces Enable High-Titer An..." refers background in this paper
...Escherichia coli BW25113 (rrnBT14 lacZWJ16 hsdR514 araBADAH33 rhaBADLD78) was designated as the wild type (16)....
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1,955 citations
"Driving Forces Enable High-Titer An..." refers background in this paper
...These low titers of heterologous 1-butanol production demonstrate the difficulty in transferring this pathway to nonnative hosts and are in sharp contrast to the high titers of related compounds, such as ethanol (50 g/liter) (27, 45), isobutanol (20 to 50 g/liter) (3, 5), and isopropanol (40 to 140 g/liter) (24), produced by recombinant E....
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1,017 citations
"Driving Forces Enable High-Titer An..." refers background in this paper
...1-Butanol, a potential fuel substitute and an important C4 chemical feedstock (30), is naturally synthesized by Clostridium species using a pathway that involves multiple coenzyme A (CoA)-activated intermediates (hereinafter called the CoAdependent pathway)....
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...Glycerol had no observable contribution, while yeast extract appeared to be an important nitrogen source that enhanced cell growth and helped lead to higher titers of 1-butanol (30)....
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"Driving Forces Enable High-Titer An..." refers background or methods or result in this paper
...The crt-hbd fragment was created by individually amplifying crt with primers crtfxba and crtrSOE and hbd with hbdfSOE and hbdrxba using pJCL60 (2) as a...
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...Detailed procedures were described previously (2)....
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...The construction of strains JCL16 (BW25113 with lacI provided on F ), JCL166, and JCL299 was described previously (2)....
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...In contrast, our previous work (2) used the BcdEtfAB complex instead of Ter....
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...Contrary to what was reported previously using the Bcd-EtfAB complex (2), where a small amount of oxygen was necessary to achieve higher 1-butanol productivity, the highest accumulation of 1-butanol was observed under fully anaerobic conditions in this case (Fig....
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536 citations
"Driving Forces Enable High-Titer An..." refers background in this paper
...5 mg/liter) (38), Lactobacillus brevis (300 mg/liter) (7), Pseudomonas putida (580 mg/liter), and Bacillus subtilis (120 mg/liter) (32)....
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...This general scheme of alleviating the anaerobic redox imbalance (22, 25, 38, 40, 42) caused by the inactivation of NADH-consuming reactions with target production pathways is applicable to systems where directed evolution is desired....
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