Drosophila protamine-like Mst35Ba and Mst35Bb are required for proper sperm nuclear morphology but are dispensable for male fertility.
Samantha Tirmarche,Shuhei Kimura,Laure Sapey-Triomphe,William J. Sullivan,Frédéric Landmann,Benjamin Loppin +5 more
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TLDR
Drosophila males homozygous for a genomic deletion covering several genes including the protamine-like genes Mst35Ba/b are surprisingly fertile, and this work precisely deleted the Mst 35B locus by homologous recombination, and it is confirmed the dispensability of Mst34B for fertility.Abstract:
During spermiogenesis, histones are massively replaced with protamines. A previous report showed that Drosophila males homozygous for a genomic deletion covering several genes including the protamine-like genes Mst35Ba/b are surprisingly fertile. Here, we have precisely deleted the Mst35B locus by homologous recombination, and we confirm the dispensability of Mst35Ba/b for fertility.read more
Citations
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Journal ArticleDOI
Prtl99C Acts Together with Protamines and Safeguards Male Fertility in Drosophila
TL;DR: Data reveal that at least three chromatin-binding proteins act together in chromatin reorganization to compact the paternal chromatin, in agreement with independent loading of these factors into sperm chromatin.
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The Cif proteins from Wolbachia prophage WO modify sperm genome integrity to establish cytoplasmic incompatibility
TL;DR: The results strongly support the Host Modification model of CI in which Cifs initially modify the paternal and maternal gametes to bestow CI-defining embryonic lethality and rescue.
Journal ArticleDOI
A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.
Emily L Rivard,Andrew G. Ludwig,Prajal H. Patel,Anna Grandchamp,Sarah E. Arnold,Alina Berger,Emilie M. Scott,Brendan J Kelly,Grace C. Mascha,Erich Bornberg-Bauer,Erich Bornberg-Bauer,Geoffrey D. Findlay +11 more
TL;DR: In this paper, the authors conducted a functional genetic screen of over 40 putative de novo genes with testis-enriched expression in Drosophila melanogaster and identified one gene, atlas, required for male fertility.
Posted ContentDOI
A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster
Emily L Rivard,Emily L Rivard,Andrew G. Ludwig,Prajal H. Patel,Anna Grandchamp,Sarah E. Arnold,Sarah E. Arnold,Alina Berger,Emilie M. Scott,Brendan J Kelly,Brendan J Kelly,Grace C. Mascha,Erich Bornberg-Bauer,Erich Bornberg-Bauer,Geoffrey D. Findlay +14 more
TL;DR: In this article, the authors conducted a functional genetic screen of over 40 putative de novo genes with testis-enriched expression in Drosophila melanogaster and identified one gene, atlas, required for male fertility.
Journal ArticleDOI
The Deadbeat Paternal Effect of Uncapped Sperm Telomeres on Cell Cycle Progression and Chromosome Behavior in Drosophila melanogaster.
TL;DR: It is shown that Drosophila melanogaster requires Deadbeat, a sperm nuclear basic protein (SNBP) that is recruited to the telomere by the TCC and is required for TCC maintenance during genome-wide chromatin remodeling, which transforms spermatids to mature sperm.
References
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Journal ArticleDOI
The protamine family of sperm nuclear proteins
TL;DR: Comparison of protamine gene and amino-acid sequences suggests that the family evolved from specialized histones through protamine-like proteins to the true protamines.
Journal ArticleDOI
Haploinsufficiency of protamine-1 or -2 causes infertility in mice.
Chunghee Cho,William D. Willis,Eugenia H. Goulding,Haesook Jung-Ha,Young-Chul Choi,Norman B. Hecht,Edward M. Eddy +6 more
TL;DR: It is found that a decrease in the amount of either protamine disrupts nuclear formation, processing of protamine-2 and normal sperm function, and that haploinsufficiency caused by a mutation in one allele of Prm1 or Prm2 prevents genetic transmission of both mutant and wild-type alleles.
Journal ArticleDOI
Chromatin dynamics during spermiogenesis.
TL;DR: This review highlights the current knowledge on post-meiotic chromatin reorganization and reveals for the first time intriguing parallels in this process in Drosophila and mammals and illustrates the possible mechanisms that lead from a histone-based chromatin to a mainly protamine-based structure during spermatid differentiation.
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Paternal DNA packaging in spermatozoa: more than the sum of its parts? DNA, histones, protamines and epigenetics.
TL;DR: The most recent research into mammalian spermatozoal chromatin composition is discussed, supporting the hypothesis that the spermatozoon delivers a novel epigenetic signature to the egg that may be crucial for normal development and some thoughts on why this signature may be required in early embryogenesis.
Journal ArticleDOI
Ends-out, or replacement, gene targeting in Drosophila
Wei J. Gong,Kent G. Golic +1 more
TL;DR: It is found that ends-out targeting can be approximately as efficient as ends-in targeting, and is likely to be generally useful for Drosophila gene targeting.