Dynamic regulatory interactions of rad51, rad52, and replication protein-a in recombination intermediates.
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203 citations
Cites background from "Dynamic regulatory interactions of ..."
...In addition, prior biochemical and genetic studies have clearly shown that Rad52 assists assembly of Rad51 filaments on RPA-bound ssDNA [24,25,26,27,28,29,33]....
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...This effect can be overcome in vitro by adding RPA after Rad51, or through the inclusion of the recombination mediator protein Rad52 (in yeast), or Brca2 (in humans) [24,25,26,27,28,29,30,31,32]....
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...If added prior to or concurrently with Rad51, then RPA out competes Rad51 for available ssDNA binding sites [24,25,26,27,28,29]....
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...the same ssDNA binding sites, and RPA binds to ssDNA more tightly than Rad51 [24,25,27]....
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...consequence, RPA can outcompete Rad51 for ssDNA binding both in vitro and in vivo, and Rad51 requires mediator proteins such as Rad52 [24,25,27,33], implying that Rad51 itself lacks an...
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180 citations
Cites background from "Dynamic regulatory interactions of ..."
...defects and likely stems from effects on RPA release from ssDNA, where Rad52 is unable to promote release of RPA from ssDNA and enable Rad51 exchange (164, 165)....
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128 citations
Cites background from "Dynamic regulatory interactions of ..."
...Formation of the hRPA–ssDNA–hRad52 complex on short oligonucleotides that can bind only one or two hRPA molecules distinguishes these proteins from their yeast counterparts, where formation of the stable yRPA–ssDNA–yRad52 complex requires multiple RPA molecules bound to the same ssDNA (59)....
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References
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"Dynamic regulatory interactions of ..." refers methods in this paper
...In the current model, DSB ends are resected to expose ssDNA with a 3′ tail that is considered to be the substrate for multiple pathways of recombination.(1,2) In vivo studies showed that RPA and Rad52 associate with the break site before Rad51 does....
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1,542 citations
"Dynamic regulatory interactions of ..." refers background in this paper
...All rights reserve many of which are widely conserved in eukaryotes, are involved in DSB repair.(1,7) Among them, Rad51, a key protein in HR-mediated repair, is being studied aggressively....
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1,454 citations