Dynamics in the plasma membrane: how to combine fluidity and order
Didier Marguet,Didier Marguet,Pierre-François Lenne,Pierre-François Lenne,Hervé Rigneault,Hervé Rigneault,Hai-Tao He,Hai-Tao He +7 more
Reads0
Chats0
TLDR
The basic concepts of Brownian diffusion and lipid domain formation in model membranes are summarized and the development of ideas and tools in this field are tracked, outlining key results obtained on the dynamic processes at work in membrane structure and assembly.Abstract:
Cell membranes are fascinating supramolecular aggregates that not only form a barrier between compartments but also harbor many chemical reactions essential to the existence and functioning of a cell. Here, it is proposed to review the molecular dynamics and mosaic organization of the plasma membrane, which are thought to have important functional implications. We will first summarize the basic concepts of Brownian diffusion and lipid domain formation in model membranes and then track the development of ideas and tools in this field, outlining key results obtained on the dynamic processes at work in membrane structure and assembly. We will focus in particular on findings made using fluorescent labeling and imaging procedures to record these dynamic processes. We will also discuss a few examples showing the impact of lateral diffusion on cell signal transduction, and outline some future methodological challenges which must be met before we can answer some of the questions arising in this field of research.read more
Citations
More filters
Journal ArticleDOI
Early life permethrin insecticide treatment leads to heart damage in adult rats.
TL;DR: The findings support the evidence that the neonatal period has a critical role in the development of heart disease in adulthood and hypothesize that the alterations observed in adult rats could depend on epigenetic changes that occurred during this period which influence gene expression throughout the rat's life, leading to alterations of certain parameters related to cardiac function.
Journal ArticleDOI
The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size
Melda Sonmez,Huseyin Yavuz Ince,Ozlem Yalcin,Vladimir Ajdžanović,Ivan Spasojevic,Herbert J. Meiselman,Oguz K. Baskurt +6 more
TL;DR: The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy to document the existence of mechanical links between RBC deformable and near-surface membrane fluidality, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of R BC deformability
Journal ArticleDOI
T-cell antigen receptor triggering and lipid rafts: a matter of space and time scales. Talking Point on the involvement of lipid rafts in T-cell activation.
TL;DR: It is argued that the key to achieving a better understanding of both processes is the ability to monitor the molecular dynamics and interactions taking place in the membrane of T cells at a spatial scale of tens to hundreds of nanometres, with a subsecond‐to‐second temporal resolution.
Journal ArticleDOI
Lateral Mobility of Nicotinic Acetylcholine Receptors on Neurons Is Determined by Receptor Composition, Local Domain, and Cell Type
TL;DR: Single-particle tracking with Quantum Dots is used to follow nicotinic acetylcholine receptors (nAChRs) on the surface of chick ciliary ganglion neurons in culture to create a system that could tailor Nicotinic signaling capabilities to specific needs of individual locations.
Journal ArticleDOI
Heterogeneity and lateral compartmentalization of plant plasma membranes.
TL;DR: This work has shown that phytosterols can induce phase separation, a prerequisite for the formation of membrane rafts, and demonstrated the importance of correct sterol composition and endocytosis for proper membrane compartmentalization in detergent-resistant membranes.
References
More filters
Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Journal ArticleDOI
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
Xavier Michalet,Fabien Pinaud,Laurent A. Bentolila,James M. Tsay,Sören Doose,J. Jack Li,Gobalakrishnan Sundaresan,Anna M. Wu,Sanjiv S. Gambhir,Sanjiv S. Gambhir,Shimon Weiss +10 more
TL;DR: The new generations of qdots have far-reaching potential for the study of intracellular processes at the single-molecule level, high-resolution cellular imaging, long-term in vivo observation of cell trafficking, tumor targeting, and diagnostics.
Journal ArticleDOI
Quantum dot bioconjugates for imaging, labelling and sensing
TL;DR: This review looks at current methods for preparing QD bioconjugates as well as presenting an overview of applications, and concludes that the potential of QDs in biology has just begun to be realized and new avenues will arise as the ability to manipulate these materials improves.
Book
Random walks in biology
TL;DR: This book is a lucid, straightforward introduction to the concepts and techniques of statistical physics that students of biology, biochemistry, and biophysics must know.
Journal ArticleDOI
A guide to choosing fluorescent proteins.
TL;DR: A unified characterization of the best available FPs provides a useful guide in narrowing down the options for biological imaging tools.