Dynamics in the plasma membrane: how to combine fluidity and order
Didier Marguet,Didier Marguet,Pierre-François Lenne,Pierre-François Lenne,Hervé Rigneault,Hervé Rigneault,Hai-Tao He,Hai-Tao He +7 more
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TLDR
The basic concepts of Brownian diffusion and lipid domain formation in model membranes are summarized and the development of ideas and tools in this field are tracked, outlining key results obtained on the dynamic processes at work in membrane structure and assembly.Abstract:
Cell membranes are fascinating supramolecular aggregates that not only form a barrier between compartments but also harbor many chemical reactions essential to the existence and functioning of a cell. Here, it is proposed to review the molecular dynamics and mosaic organization of the plasma membrane, which are thought to have important functional implications. We will first summarize the basic concepts of Brownian diffusion and lipid domain formation in model membranes and then track the development of ideas and tools in this field, outlining key results obtained on the dynamic processes at work in membrane structure and assembly. We will focus in particular on findings made using fluorescent labeling and imaging procedures to record these dynamic processes. We will also discuss a few examples showing the impact of lateral diffusion on cell signal transduction, and outline some future methodological challenges which must be met before we can answer some of the questions arising in this field of research.read more
Citations
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Curvature-coupling dependence of membrane protein diffusion coefficients
TL;DR: In this study, the lateral diffusion of a protein interacting with the curvature of the membrane is considered and it is found that this curvature coupling substantially enhances the diffusion coefficient.
Journal ArticleDOI
Membrane Microdomains and Cytoskeleton Organization Shape and Regulate the IL-7 Receptor Signalosome in Human CD4 T-cells
Blanche Tamarit,Blanche Tamarit,Florence Bugault,Anne-Hélène Pillet,Anne-Hélène Pillet,Vincent Lavergne,Pascal Bochet,Pascal Bochet,Nathalie Garin,Ulf Schwarz,Jacques Thèze,Thierry Rose +11 more
TL;DR: Key roles played by membrane microdomains and cytoskeleton transient organization in the IL-7-regulated JAK/STAT signaling pathway are described by stimulated emission depletion microscopy.
Journal ArticleDOI
Dynamic submicroscopic signaling zones revealed by pair correlation tracking and localization microscopy.
TL;DR: The results suggest that confined diffusion zones in the plasma membrane are employed as transient platforms for the assembly of signaling complexes within dynamic submicroscopic zones.
Journal ArticleDOI
Cell surface-localized imaging and sensing.
TL;DR: In this review, recent advances that focus on the visualization of various cell surface structures/dynamics and accurate monitoring of the microenvironment of the cell surface are summarized.
Journal ArticleDOI
Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments
David Richter,Ignacio Moraga,Hauke Winkelmann,Oliver Birkholz,Stephan Wilmes,Markos Schulte,Michael Kraich,Hella Kenneweg,Oliver Beutel,Philipp Selenschik,Dirk Paterok,Martynas Gavutis,Thomas Schmidt,K. Christopher Garcia,Thomas Müller,Jacob Piehler +15 more
TL;DR: Analysis of the molecular and cellular determinants governing the assembly of the type II interleukin-4 receptor suggests that kinetic trapping of receptor dimers in actin-dependent microcompartments sustains robust receptor dimerization and signalling.
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