Dynamics in the plasma membrane: how to combine fluidity and order
Didier Marguet,Didier Marguet,Pierre-François Lenne,Pierre-François Lenne,Hervé Rigneault,Hervé Rigneault,Hai-Tao He,Hai-Tao He +7 more
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TLDR
The basic concepts of Brownian diffusion and lipid domain formation in model membranes are summarized and the development of ideas and tools in this field are tracked, outlining key results obtained on the dynamic processes at work in membrane structure and assembly.Abstract:
Cell membranes are fascinating supramolecular aggregates that not only form a barrier between compartments but also harbor many chemical reactions essential to the existence and functioning of a cell. Here, it is proposed to review the molecular dynamics and mosaic organization of the plasma membrane, which are thought to have important functional implications. We will first summarize the basic concepts of Brownian diffusion and lipid domain formation in model membranes and then track the development of ideas and tools in this field, outlining key results obtained on the dynamic processes at work in membrane structure and assembly. We will focus in particular on findings made using fluorescent labeling and imaging procedures to record these dynamic processes. We will also discuss a few examples showing the impact of lateral diffusion on cell signal transduction, and outline some future methodological challenges which must be met before we can answer some of the questions arising in this field of research.read more
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Plasma membrane asymmetry of lipid organization: fluorescence lifetime microscopy and correlation spectroscopy analysis
TL;DR: The results show that the combination of FLIM and ITIR-FCS with specific fluorescent lipid analogs is a powerful tool for investigating lateral and transbilayer characteristics of plasma membrane in live cell lines.
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Proteomics and the dynamic plasma membrane: Quo Vadis?
TL;DR: Vadis et al. as discussed by the authors reviewed the recent progress in MS-based plasma membrane proteomics by presenting key examples from eukaryotic systems, including mammals, yeast and plants.
Journal ArticleDOI
Shear and Compression Rheology of Langmuir Monolayers of Natural Ceramides: Solid Character and Plasticity
Iván López-Montero,Elisa R. Catapano,Gabriel Espinosa,Gabriel Espinosa,Laura R. Arriaga,Dominique Langevin,Francisco Monroy,Francisco Monroy +7 more
TL;DR: The present work addresses the fundamental question of membrane elasticity of ceramide layers with a special focus on the plastic regime and permits to univocally classify ceramide monolayers as 2D solids able to undergo plastic deformations, at the difference of typical fluid lipid monolayer.
Journal ArticleDOI
Aqueous-filled polymer microcavity arrays: versatile & stable lipid bilayer platforms offering high lateral mobility to incorporated membrane proteins.
TL;DR: Fluorescence Lifetime Correlation Spectroscopy confirmed the pore suspended lipid bilayer exhibits diffusion coefficients comparable to free-standing vesicles in solution, and the bilayer modified arrays are highly reproducible and stable over days.
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Heterogeneous localisation of membrane proteins in Staphylococcus aureus
Felix Weihs,Katarzyna Wacnik,Robert D. Turner,Siân Culley,Ricardo Henriques,Ricardo Henriques,Simon J. Foster +6 more
TL;DR: Analysis of the localisation of key membrane proteins found a link to cell division and a possible role for FtsZ in functions not strictly associated with septum formation, and novel colocalisation between phospholipid biosynthesis enzymes and the respiratory protein CydB revealing a likely larger network of partners.
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