Dynamics in the plasma membrane: how to combine fluidity and order
Didier Marguet,Didier Marguet,Pierre-François Lenne,Pierre-François Lenne,Hervé Rigneault,Hervé Rigneault,Hai-Tao He,Hai-Tao He +7 more
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TLDR
The basic concepts of Brownian diffusion and lipid domain formation in model membranes are summarized and the development of ideas and tools in this field are tracked, outlining key results obtained on the dynamic processes at work in membrane structure and assembly.Abstract:
Cell membranes are fascinating supramolecular aggregates that not only form a barrier between compartments but also harbor many chemical reactions essential to the existence and functioning of a cell. Here, it is proposed to review the molecular dynamics and mosaic organization of the plasma membrane, which are thought to have important functional implications. We will first summarize the basic concepts of Brownian diffusion and lipid domain formation in model membranes and then track the development of ideas and tools in this field, outlining key results obtained on the dynamic processes at work in membrane structure and assembly. We will focus in particular on findings made using fluorescent labeling and imaging procedures to record these dynamic processes. We will also discuss a few examples showing the impact of lateral diffusion on cell signal transduction, and outline some future methodological challenges which must be met before we can answer some of the questions arising in this field of research.read more
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Myelin basic protein-dependent plasma membrane reorganization in the formation of myelin
Dirk Fitzner,Anja Schneider,Anja Schneider,Angelika Kippert,Angelika Kippert,Wiebke Möbius,Katrin I. Willig,Stefan W. Hell,Gertrude Bunt,Katharina Gaus,Mikael Simons,Mikael Simons +11 more
TL;DR: The data demonstrate that neurons induce the condensation of the myelin‐forming bilayer in oligodendrocytes and that MBP is involved in this process of plasma membrane rearrangement and propose that this mechanism is essential for myelin to perform its insulating function during nerve conduction.
Journal ArticleDOI
Lipid changes in the aged brain: effect on synaptic function and neuronal survival.
TL;DR: The current understanding of the roles of lipids in neuronal aging is described and discussed, with special attention to their influence on membrane fusion, neurotransmitter receptor dynamics and survival/death signaling pathways.
Journal ArticleDOI
Use of Bodipy-labeled sphingolipid and cholesterol analogs to examine membrane microdomains in cells
TL;DR: Bodipy-sphingolipids may be useful in future studies to examine the relationship between membrane domains at the cell surface and domains enriched in other lipids and proteins on the inner leaflet of the plasma membrane.
Journal ArticleDOI
Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking
Yasuhiro Umemura,Marija Vrljic,Stefanie Y. Nishimura,Takahiro K. Fujiwara,Kenichi G. N. Suzuki,Akihiro Kusumi +5 more
TL;DR: The results indicate that the actin-based membrane skeleton influences the diffusion of both transmembrane and GPI-anchored proteins.
Journal ArticleDOI
Tracking microdomain dynamics in cell membranes
Charles A. Day,Anne K. Kenworthy +1 more
TL;DR: A brief summary of the classes of domains that have been characterized to date is provided, focusing on recent efforts to identify the properties of lipid rafts in cells through measurements of protein and lipid diffusion.
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