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Journal ArticleDOI

Effect of carbon sources on the synthesis of pectinase by Aspergilli

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TLDR
A. foetidus, NCIM 510, was the only one among the organisms studied, that responded well to the medium containing pectin in the absence of additional sugars supplied in the medium.
Abstract
The synthesis of pectinase is investigated using six species of Aspergillus, with five media differing either in their carbon sources or level of carbon source(s). Five of the six species used, synthesized appreciable amounts of pectinase in the media containing sugars. Pectinase synthesis was highest for A. niger, NCIM 548, with all the sugar containing media. A. foetidus, NCIM 510, was the only one among the organisms studied, that responded well to the medium containing pectin in the absence of additional sugars supplied in the medium.

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Citations
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Journal ArticleDOI

Purification and biochemical properties of microbial pectinases—a review

TL;DR: This short review highlights progress on purification and understanding the biochemical aspects of microbial pectinases.
Journal ArticleDOI

Production of pectolytic enzymes – a review

TL;DR: In this article, a review of the production of pectolytic enzymes using different carbon sources is presented and the effect of operating parameters such as temperature, aeration rate, agitation and type of fermentation is discussed.
Journal ArticleDOI

Optimising growth conditions for the pectinolytic activity of Kluyveromyces wickerhamii by using response surface methodology.

TL;DR: The effects of temperature and pH were the most significant factors in influencing enzyme production.
Journal ArticleDOI

Utilisation of whole wheat flour for the production of extracellular pectinases by some fungal strains

TL;DR: In this paper, the possibility of producing pectinases by Rhizopus stolonifer and Aspergillus awamori, using cereal raw materials as substrate, was investigated.
Journal ArticleDOI

Multiresponse analysis of microbiological parameters affecting the production of pectolytic enzymes by Aspergillus niger: a statistical view

TL;DR: In order to develop a two-stage inoculum possessing the best conditions for the maximum production of all three enzymes, a multiresponse analysis was carried out using a generalized distance approach.
References
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Book ChapterDOI

Assay methods for pectic enzymes

TL;DR: Three assay procedures are described that permit quantitative assay of hydrolytic and β-eliminative pectic enzymes, respectively, and can guide purification strategies, particularly when used in conjunction with titration curves.
Journal ArticleDOI

Effects of different carbon sources on the synthesis of pectinase by Aspergillus niger in submerged and solid state fermentations

TL;DR: Results indicate that regulatory phenomena, such as induction-repression or activation-inhibition, related to pectinase synthesis by A. niger CH4 are different in the two types of fermentation.
Journal ArticleDOI

Strain and process for production of polygalacturonase

TL;DR: A process was developed for the production of polygalacturonase by Aspergillus niger and its mutant VTT-D-77050 and the enzyme mixture produced in one pilot fermentation was used successfully for improving the cloud stability of fruit nectars.
Journal ArticleDOI

Research on pectin depolymerases in the sixties ‐ a literature review

TL;DR: This review deals mainly with the literature which has appeared since the discovery of pectin lyases in 1960 and it is emphasized that the choice of substrates and reaction conditions, as well as that of methods of analysis, to a large extent influence the values found for a number of enzyme properties.
Journal ArticleDOI

Catabolite repression of hydrolases in Aspergillus niger

TL;DR: It is suggested that catabolite repression of polygalacturonase occurs at the level of translation and that of acid protease at transcription in an adenine-requiring mutant of Aspergillus niger.
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