Journal ArticleDOI
Effect of comprehensive lifestyle changes on telomerase activity and telomere length in men with biopsy-proven low-risk prostate cancer: 5-year follow-up of a descriptive pilot study.
Dean Ornish,Jue Lin,June M. Chan,Elissa S. Epel,Colleen Kemp,Gerdi Weidner,Ruth Marlin,Steven J. Frenda,Mark Jesus M. Magbanua,Jennifer Daubenmier,Ivette S. Estay,Nancy K. Hills,Nita Chainani-Wu,Peter R. Carroll,Elizabeth H. Blackburn +14 more
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The comprehensive lifestyle intervention followed with a programme of comprehensive lifestyle changes was associated with increases in relative telomere length after 5 years of follow-up, compared with controls, in this small pilot study.Abstract:
Summary Background Telomere shortness in human beings is a prognostic marker of ageing, disease, and premature morbidity. We previously found an association between 3 months of comprehensive lifestyle changes and increased telomerase activity in human immune-system cells. We followed up participants to investigate long-term effects. Methods This follow-up study compared ten men and 25 external controls who had biopsy-proven low-risk prostate cancer and had chosen to undergo active surveillance. Eligible participants were enrolled between 2003 and 2007 from previous studies and selected according to the same criteria. Men in the intervention group followed a programme of comprehensive lifestyle changes (diet, activity, stress management, and social support), and the men in the control group underwent active surveillance alone. We took blood samples at 5 years and compared relative telomere length and telomerase enzymatic activity per viable cell with those at baseline, and assessed their relation to the degree of lifestyle changes. Findings Relative telomere length increased from baseline by a median of 0·06 telomere to single-copy gene ratio (T/S)units (IQR–0·05 to 0·11) in the lifestyle intervention group, but decreased in the control group (−0·03 T/S units, −0·05 to 0·03, difference p=0·03). When data from the two groups were combined, adherence to lifestyle changes was significantly associated with relative telomere length after adjustment for age and the length of follow-up (for each percentage point increase in lifestyle adherence score, T/S units increased by 0·07, 95% CI 0·02–0·12, p=0·005). At 5 years, telomerase activity had decreased from baseline by 0·25 (–2·25 to 2·23) units in the lifestyle intervention group, and by 1·08 (–3·25 to 1·86) units in the control group (p=0·64), and was not associated with adherence to lifestyle changes (relative risk 0·93, 95% CI 0·72–1·20, p=0·57). Interpretation Our comprehensive lifestyle intervention was associated with increases in relative telomere length after 5 years of follow-up, compared with controls, in this small pilot study. Larger randomised controlled trials are warranted to confirm this finding. Funding US Department of Defense, NIH/NCI, Furlotti Family Foundation, Bahna Foundation, DeJoria Foundation, Walton Family Foundation, Resnick Foundation, Greenbaum Foundation, Natwin Foundation, Safeway Foundation, Prostate Cancer Foundation.read more
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Identification of seven loci affecting mean telomere length and their association with disease
Veryan Codd,Christopher P. Nelson,Eva Albrecht,Massimo Mangino,Joris Deelen,Jessica L. Buxton,Jouke-Jan Hottenga,Krista Fischer,Tõnu Esko,Ida Surakka,Linda Broer,Dale R. Nyholt,Irene Mateo Leach,Perttu Salo,Sara Hägg,Mary K. Matthews,Jutta Palmen,Giuseppe Danilo Norata,Paul F. O'Reilly,Danish Saleheen,Najaf Amin,Anthony J. Balmforth,Marian Beekman,Rudolf A. de Boer,Stefan Böhringer,Peter S. Braund,Paul Burton,Anton J. M. de Craen,Matthew Denniff,Yanbin Dong,Konstantinos Douroudis,Elena Dubinina,Johan G. Eriksson,Katia Garlaschelli,Dehuang Guo,Anna-Liisa Hartikainen,Anjali K. Henders,Jeanine J. Houwing-Duistermaat,Laura Kananen,Lennart C. Karssen,Johannes Kettunen,Norman Klopp,Vasiliki Lagou,Elisabeth M. van Leeuwen,Pamela A. F. Madden,Reedik Maegi,Patrik K. E. Magnusson,Satu Männistö,Mark I. McCarthy,Sarah E. Medland,Evelin Mihailov,Grant W. Montgomery,Ben A. Oostra,Aarno Palotie,Annette Peters,Helen Pollard,Anneli Pouta,Inga Prokopenko,Samuli Ripatti,Veikko Salomaa,H. Eka D. Suchiman,Ana M. Valdes,Niek Verweij,Ana Viñuela,Xiaoling Wang,H-Erich Wichmann,Elisabeth Widen,Gonneke Willemsen,Margaret J. Wright,Kai Xia,Xiangjun Xiao,Dirk J. van Veldhuisen,Alberico L. Catapano,Martin D. Tobin,Alistair S. Hall,Alexandra I. F. Blakemore,Wiek H. van Gilst,Haidong Zhu,Jeanette Erdmann,Muredach P. Reilly,Sekar Kathiresan,Heribert Schunkert,Philippa J. Talmud,Nancy L. Pedersen,Markus Perola,Willem H. Ouwehand,Jaakko Kaprio,Nicholas G. Martin,Cornelia M. van Duijn,Iris Hovatta,Christian Gieger,Andres Metspalu,Dorret I. Boomsma,Marjo-Riitta Järvelin,P. Eline Slagboom,John R Thompson,Tim D. Spector,Pim van der Harst,Nilesh J. Samani +98 more
TL;DR: In this article, a genome-wide meta-analysis of 37,684 individuals with replication of selected variants in an additional 10,739 individuals was carried out to identify seven loci, including five new loci associated with mean leukocyte telomere length (LTL).
Journal ArticleDOI
Epigenetic clock analysis of diet, exercise, education, and lifestyle factors
Austin Quach,Morgan E. Levine,Toshiko Tanaka,Ake T. Lu,Brian H. Chen,Luigi Ferrucci,Beate Ritz,Stefania Bandinelli,Marian L. Neuhouser,Jeannette M. Beasley,Linda Snetselaar,Robert B. Wallace,Philip S. Tsao,Devin Absher,Themistocles L. Assimes,James D. Stewart,Yun Li,Lifang Hou,Andrea A. Baccarelli,Eric A. Whitsel,Steve Horvath +20 more
TL;DR: Overall, the epigenetic age analysis of blood confirms the conventional wisdom regarding the benefits of eating a high plant diet with lean meats, moderate alcohol consumption, physical activity, and education, as well as the health risks of obesity and metabolic syndrome.
Journal ArticleDOI
Network pharmacology applications to map the unexplored target space and therapeutic potential of natural products
TL;DR: It is argued that a network pharmacology approach would enable an effective mapping of the yet unexplored target space of natural products, hence providing a systematic means to extend the druggable space of proteins implicated in various complex diseases.
Journal ArticleDOI
Psychiatric disorders and leukocyte telomere length: Underlying mechanisms linking mental illness with cellular aging.
Daniel Lindqvist,Elissa S. Epel,Synthia H. Mellon,Brenda W.J.H. Penninx,Dóra Révész,Josine E. Verhoeven,Victor I. Reus,Jue Lin,Laura Mahan,Christina M. Hough,Rebecca Rosser,F. Saverio Bersani,Elizabeth H. Blackburn,Owen M. Wolkowitz +13 more
TL;DR: A deeper understanding of cellular aging in psychiatric illnesses could lead to re-conceptualizing them as systemic illnesses with manifestations inside and outside the brain and could identify new treatment targets.
Journal ArticleDOI
Can physical activity ameliorate immunosenescence and thereby reduce age-related multi-morbidity?
TL;DR: In this article, the authors discuss how physical activity can prevent or ameliorate age-related multi-morbidity by boosting immune function, and consider whether physical activity could improve immunotherapy outcomes in agerelated conditions such as cancer.
References
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Telomere measurement by quantitative PCR
TL;DR: A primer pair is presented that eliminates the problem of presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, allowing simple and rapid measurement of telomere length in a closed tube, fluorescence-based assay.
Journal ArticleDOI
Accelerated telomere shortening in response to life stress
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TL;DR: Evidence is provided that psychological stress--both perceived stress and chronicity of stress--is significantly associated with higher oxidative stress, lower telomerase activity, and shorter telomere length, in peripheral blood mononuclear cells from healthy premenopausal women.
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Can lifestyle changes reverse coronary heart disease?: The Lifestyle Heart Trial
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TL;DR: Comprehensive lifestyle changes may be able to bring about regression of even severe coronary atherosclerosis after only 1 year, without use of lipid-lowering drugs.
Journal ArticleDOI
Reproducibility and Validity of an Expanded Self-Administered Semiquantitative Food Frequency Questionnaire among Male Health Professionals
Eric B. Rimm,Edward Giovannucci,Meir J. Stampfer,Meir J. Stampfer,Graham A. Colditz,Graham A. Colditz,Lisa B. Litin,Walter C. Willett,Walter C. Willett +8 more
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Trypan blue exclusion test of cell viability.
TL;DR: The dye exclusion test as discussed by the authors is used to determine the number of viable cells present in a cell suspension, based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not.
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