Effect of Δ9-tetrahydrocannabinol on monoamine oxidase activity of rat tissues in vivo
About: This article is published in Biochemical Pharmacology.The article was published on 1975-08-01. It has received 14 citations till now. The article focuses on the topics: Monoamine oxidase B & Monoamine oxidase.
Citations
More filters
••
167 citations
••
TL;DR: Investigation of in vitro effects of cannabinoids on the activity of monoamine oxidase found decrease of MAO activity may play a role in some effects of cannabinoid on serotonergic, noradrenergic, and dopaminergic neurotransmission.
Abstract: Brain monoamines are involved in many of the same processes affected by neuropsychiatric disorders and psychotropic drugs, including cannabinoids. This study investigated in vitro effects of cannabinoids on the activity of monoamine oxidase (MAO), the enzyme responsible for metabolism of monoamine neurotransmitters and affecting brain development and function. The effects of the phytocannabinoid Δ9-tetrahydrocannabinol (THC), the endocannabinoid anandamide (N-arachidonoylethanolamide [AEA]), and the synthetic cannabinoid receptor agonist WIN 55,212-2 (WIN) on the activity of MAO were measured in a crude mitochondrial fraction isolated from pig brain cortex. Monoamine oxidase activity was inhibited by the cannabinoids; however, higher half maximal inhibitory concentrations (IC50) of cannabinoids were required compared to the known MAO inhibitor iproniazid. The IC50 was 24.7 μmol/l for THC, 751 μmol/l for AEA, and 17.9 μmol/l for WIN when serotonin was used as substrate (MAO-A), and 22.6 μmol/l for THC, 1,668 μmol/l for AEA, and 21.2 μmol/l for WIN when phenylethylamine was used as substrate (MAO-B). The inhibition of MAOs by THC was noncompetitive. N-Arachidonoylethanolamide was a competitive inhibitor of MAO-A and a noncompetitive inhibitor of MAO-B. WIN was a noncompetitive inhibitor of MAO-A and an uncompetitive inhibitor of MAO-B. Monoamine oxidase activity is affected by cannabinoids at relatively high drug concentrations, and this effect is inhibitory. Decrease of MAO activity may play a role in some effects of cannabinoids on serotonergic, noradrenergic, and dopaminergic neurotransmission.
79 citations
Cites background from "Effect of Δ9-tetrahydrocannabinol o..."
...Early studies found different effects of cannabinoids on MAO activity; THC elicits inhibitory (Mazor et al. 1982), stimulatory (Banerjee et al. 1975; Chakrabarty et al. 1976; Banerji et al. 1977; Gawienowski et al. 1982), or no effects (Clarke and Jandhyala 1977; Schurr and Rigor 1984)....
[...]
...1982), stimulatory (Banerjee et al. 1975; Chakrabarty et al. 1976; Banerji et al. 1977; Gawienowski et al. 1982), or no effects (Clarke and Jandhyala 1977; Schurr and Rigor 1984)....
[...]
••
TL;DR: Mean platelet monoamine oxidase activity in 26 consecutively-studied male marijuana smokers was significantly lower than in a comparable group of non-marijuana smoking males, and the level of current marijuana use reported by the subjects was significantly and inversely correlated with MAO activity.
45 citations
••
TL;DR: The aim of these studies was to establish a baseline level of understanding of the role of adrenergic pathways in the development and aging of cyclic GMP, as well as to provide a baseline for the regulation of phosphodiesterases and inhibitors.
34 citations
••
TL;DR: It is suggested that some of the behavioural effects of cannabis administered under stressful conditions may be related to alterations in striatal dopamine metabolism, which is consistent with increased dopamine reuptake in striatum produced by this combination of THC and novel environment.
32 citations
References
More filters
••
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.
15,717 citations
••
TL;DR: Administration of pure 1-δ9-tetrahydrocannabinol to mice had the following dose-dependent nzeurochemical and behavioral effects: a slight but significant increase in concentrations of 5-hydroxytryptamine in whole brain; a decrease in concentration of norepinephrine in brain after administration of low doses and an increase after high doses.
Abstract: Administration of pure 1-delta(9)-tetrahydrocannabinol to mice had the following dose-dependent nzeurochemical and behavioral effects: a slight but significant increase in concentrations of 5-hydroxytryptamine in whole brain; a decrease in concentration of norepinephrine in brain after administration of low doses and an increase after high doses; diminished spontaneous activity, mloderate hypothermnia, hypersetisitivity to tactile and auditory stimiuli, and ataxia after low doses; and sedation, pronounced hypothermia, and markedly diminished spon taneous activity and reactivity after high doses. The duration of the effects on body temperature and spontaneous activity correlated generally with the changes in brain amines. The characteristic changes in brain amines do not correspond exactly to those observed with other psychotropic drugs.
196 citations
••
TL;DR: Michael, S. E. & Charlwood, P. A. (1958).
Abstract: Michael, S. E. (1958). Ab8tr. Comm. 4thint,. Congr. Biochem., Vienna, no. 2-106, p. 28. Pedersen, K. 0. (1958). J. phys. Chem. 62, 1282. Popjaik, G. & McCarthy, E. F. (1946). Biochem. J. 40, 789. Ram, J. S. & Maurer, P. H. (1958). Arch. Biochem. Biophys. 76, 28. Reichmann, M. E. & Charlwood, P. A. (1954). Canad. J. Chem. 32, 1092. Schwert, G. W. (1957). J. Amer. chem. Soc. 79, 139. Smith, D. B., Wood, G. C. & Charlwood, P. A. (1956). Canad. J. Chem. 34, 364. Svedberg, T. & Pedersen, K. 0. (1940). The Ultracentrifuge. Oxford University Press. Warren, R. L. & Charlwood, P. A. (1953). Nature, Lond., 171, 353. Wieme, R. J. (1959). Clin. chim. Acta, 4, 317. Williams, J. W., van Holde, K. E., Baldwin, R. L. & Fujita, H. (1958). Chem. Rev. 58, 715.
142 citations
••
TL;DR: The differences between the physicochemical properties of platelet MAO and isoenzymes from other anatomical sites suggests that the platelet enzyme is unlikely to provide a satisfactory index of in vivo activity in the whole organism.
105 citations