Effect of Extracts and Components of Black Tea on the Activity of β- Glucuronidase, Lipase,α-Amylase,α-Glucosidase: An in vitro Study
TL;DR: This in vitro study reveals that BT extract and the phenolic components may have potential benefit for controlling liver disorders, obesity and diabetes.
Abstract: The objective of the present study was to determine the inhibitory properties of the infusions and decoctions of four samples of Indian black tea (BT) and some of the tea flavonoids, gallic acid and caffeine, in vitro, against β- glucuronidase, lipase,α-amylase and α-glucosidase. These enzymes are related to liver disorders, obesity and diabetes. The phenolic constituents and caffeine content in the BT extracts were measured quantitatively by HPLC. It was noted that both infusion and decoction of BT had β-glucuronidase inhibitory property close to that of silymarin. Epigallocatechin gallate, epicatechin, and gallocatechin showed higher β-glucuronidase inhibitory activity than that of BT extracts and silymarin. BT extracts also inhibited the enzyme lipase. Thearubigin, theaflavin, epigallocatechin gallate, epicatechin and gallocatechin showed significant lipase inhibitory properties that were superior to the drug orlistat. The tea extracts, flavonoids and gallic acid inhibited the activity of α-amylase and α-glucosidase. Theaflavin and catechin had α-amylase inhibitory activity better than that of acarbose. This in vitro study reveals that BT extract and the phenolic components may have potential benefit for controlling liver disorders, obesity and diabetes.
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TL;DR: The present experiment reports the importance of both primary and secondary metabolites for the bioactivity of plant extracts and identifies the inhibitors of α-amylase and α-glucosidase using metabolomics and chemometric approaches.
Abstract: Achras sapota L. fruit extracts have previously been reported to have activities against the carbohydrate-digesting enzymes α-amylase and α-glucosidase. The present work was aimed to identify the inhibitors of α-amylase and α-glucosidase using metabolomics and chemometric approaches. Metabolite profile of Achras sapota fruit extracts at different stages of post-harvest ripening was analyzed using GC–MS-based metabolomics approach. The extracts were also assayed for their properties to inhibit the carbohydrate-digesting enzymes α-amylase and α-glucosidase in vitro. The fruit extracts just after harvesting showed maximum activities against the enzymes α-amylase and α-glucosidase. The activities declined with post-harvest ripening. It was observed from the chemometric analysis that gallic acid, chlorogenic acid, benzene-1,2,4-triol, and d-malic acid are responsible for high α-amylase inhibitory activity. Gallic acid and chlorogenic acid are positively correlated with high α-glucosidase inhibitory activity. Inhibition of the two enzymes by gallic acid, benzene-1,2,4-triol, chlorogenic acid, and α-amylase by d-malic acid supported the findings. The present experiment reports the importance of both primary and secondary metabolites for the bioactivity of plant extracts.
26 citations
Additional excerpts
...α-Amylase inhibition by chlorogenic acid and gallic acid was previously reported [19, 20]....
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TL;DR: The activities of the flower extracts against the enzymes suggest that they may have beneficial effects in controlling diseases, and the chemical constituents present in the methanol extracts were profiled following GC-MS based metabolomics approach.
Abstract: The flower extracts of two cultivated varieties of Musa X paradisiaca viz. Kanthali and Kancha Kela were assayed against the enzymes α-amylase, α-glucosidase, acetylcholinesterase, β-glucuronidase and Angiotensin I Converting Enzyme, which are related to different diseased states like diabetes, Alzheimer's disease, hepatic disorder and cardiovascular diseases, respectively. The extracts inhibited all the enzymes except α-amylase by Musa X paradisiaca cv. Kanthali. Significantly high activities were observed in both the cultivated varieties for inhibiting the enzymes β-glucuronidase and Angiotensin I Converting Enzyme with respect to the known inhibitors of such enzymes silymarin and Lisinopril, respectively. The chemical constituents present in the methanol extracts were profiled following GC-MS based metabolomics approach. Eleven organic acids, seven sugars and sugar alcohols, six fatty acids, three phenols, two amino acids and a sterol could be identified.
Practical Applications
The male flowers of Musa X paradisiaca are cooked as vegetable in South-East Asia. The flowers are also useful in diabetes. Enzyme inhibitors have properties to prevent or control different noninfectious diseases. So, the flower extracts of two varieties of the plant were assayed for their activities against the glycosidases, acetylcholinesterase, β-glucuronidase and Angiotensin I Converting Enzyme. The activities of the flower extracts against the enzymes suggest that they may have beneficial effects in controlling diseases.
17 citations
TL;DR: In this paper, anti-lipase and antioxidant activities of 5 types of tea produced by Ltd “Geoplant”, Georgia, were studied and the results showed that green traditional and black tea showed the highest level of lipase inhibition, i.e. 70.6% and 70.3% per mL (11.7% per mg dry matter) of infusion.
Abstract: Anti-lipase and antioxidant activities of 5 types of tea produced by Ltd “Geoplant”, Georgia, were studied. Traditional Green and Jasmine Blossom revealed the highest antioxidant activity – 2.35 and 2.32 mg of ascorbic acid equivalents (AAE) per mL of tea infusion made according to the usual method used by consumers. Intermediate antioxidant activity was found for black tea – 1.24 mg of AAE per mL of tea infusion. Black tea with fruits had 0.53 mg of AAE per mL of tea infusion. Alpine Berry infusion had the least antioxidant activity – 0.35 mg of AAE per mL of tea infusion. Antioxidant activities of teas were in correlation with total polyphenol contents in tea infusions with R2 = 0.81. Green traditional and black tea showed the highest level of lipase inhibition, i.e. 70.6% and 70.3% per mL (11.7% per mg dry matter) of infusion. The values were not statistically significantly different (p
16 citations
TL;DR: It is suggested that the metabolites present in the fruit mesocarp tissue showed the potential antioxidant activity and properties to inhibit the enzymes α-amylase and α-glucosidase.
Abstract: Phoenix sylvestris is an underutilized seasonal fruit in West Bengal, India. Methanol extract and extracts after alkaline hydrolysis of the mesocarp tissue of full-mature edible fruits of P. sylvestris were analyzed by GC-MS following a metabolomics approach. The fractions were tested for their antioxidant and inhibitory properties against the two key enzymes involved in diabetes, α-amylase, and α-glucosidase. Total 71 metabolites belonging to organic acids, amino acids, sugars, sugar alcohols, fatty acids, and phenols were identified in the methanol extract and in fractions after saponification. All the extracts and fractions showed high antioxidant, α-glucosidase, and α-amylase inhibitory activities. Sugars like raffinose (IC50 = 0.36 μM), sucrose (IC50 = 0.51 μM), trehalose (IC50 = 0.85 μM), and phenols like taxifolin (IC50 = 0.31 μM), benzoic acid (IC50 = 2.74 μM) inhibited only the enzyme α-amylase. Phenolic components which inhibited both the enzymes were caffeic acid (IC50 = 1.42 μM for α-a...
10 citations
Cites background from "Effect of Extracts and Components o..."
...Catechin was previously reported to have anti-amylase and antiglucosidase properties.([31]) Higher glycosidase inhibitory properties of SI and SII fractions are probably due to the presence of higher concentrations of fatty acids and phenolic compounds in those fractions than that of the methanol soluble fraction....
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TL;DR: In this paper, the authors compared chemical composition and angiotensin I-converting enzyme inhibitory properties of infusion and decoction of four samples of black tea and found that both infusion anddecoction inhibited the enzyme.
Abstract: Angiotensin I-converting enzyme inhibitors are used as therapeutic agents for the treatment of hypertension. Regular consumption of black tea (Camellia sinensis (L.) Kuntze, Theaceae) has been reported to lower blood pressure. The aims of the present work were to compare chemical composition and angiotensin I-converting enzyme inhibitory properties of infusion and decoction of four samples of black tea. GC/MS based metabolomics approach helped in identification of fifty-one metabolites including ten organic acids, one inorganic acid, sixteen amino acids, two sugars, five sugar alcohols, fifteen phenols and flavonoids, two fatty acids from infusions and decoctions of four black tea samples. Partial least squares discriminant analysis and orthogonal partial least squares discriminant analysis models showed good classification among the two groups, diffusion and infusion, based on metabolites. Both infusion and decoction inhibited the enzyme. However, the activity differed with samples. Multivariate analysis also segregated extracts on the basis of activity. Thearubigin, theaflavin, catechin inhibited the enzyme. Epicatechin, epigallocatechin gallate, gallic acid, caffeine showed lower activity.
6 citations
Cites background or methods from "Effect of Extracts and Components o..."
...T extracts analyzed by HPLC (Ray et al., 2014)....
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...This is an open access article under the CC BY-NC-ND license (http:// e Farm ( c a M P s fi I p f a R l h e N ( m e m p f u a P b e w f n P r s fi f w f s G l e d ( i a N a t m S. Ray et al. / Revista Brasileira d In India black tea is consumed either as infusion or as decoction Ray et al., 2014)....
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...I, infusion; ahati infusion; ND, Nilgiri decoction; NI, Nilgiri infusion; SD, Siliguri decoction; SI, some compounds e.g. different theaflavins identified earlier by HPLC method (Ray et al., 2014) could not be identified by the method used during the present library assisted GC–MS analysis....
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...different theaflavins identified earlier by HPLC method (Ray et al., 2014) could not be identified by the method used during the present library assisted GC–MS analysis....
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...All the solvents sed for GC/MS were of HPLC grade....
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