Journal Article•
Effect of recombinant human basic fibroblast growth factor on acute inflammation in mice and rats.
TL;DR: Recombinant human bFGF has an anti-inflammatory effect and its mechanisms are related to the inhibition of NOS activity, reduction of NO, MDA, and PGE(2) content, and increase of SOD activity.
Abstract: Aim: To investigate the anti-inflammatory effects of recombinant human basic fibroblast growth factor (rh-bFGF).
Methods: Several inflammation models such as croton oil-induced ear swelling, carrageenan-induced hind paw edema, and acute peritonitis in rats or mice were prepared. Superoxide dismutase (SOD) activity was measured by hydroxyamine method, nitric oxide (NO) concentration by Griess reaction assay, nitric oxide synthase (NOS) activity by NADPH-diaphoras stain assay, N-acetyl-beta- D-glucosaminidase (NAG) activity by colorimetry, prostaglandin E2 (PGE2) production by radioimmunoassay (RIA), malondialdehyde (MDA) content by thiobarbituric acid (TBA) fluorescence technique, and protein content by Coomassie brilliant blue method in peritoneal exudate in rats.
Results: Recombinant human bFGF 2, 4 kU/kg im inhibited croton oil-induced ear swelling and carrageenan-induced paw edema in mice. In addition, rh-bFGF 2, 4 kU/kg im reduced neutrophil counts in the rat peritoneal exudate, and lessened protein content in peritoneal exudate in rats and mice. In the rat peritonitis induced by carrageenan, rh-bFGF 4 kU/kg decreased the MDA and NO levels, inhibited the NOS activity, augmented the SOD activity, and lowered the production of PGE(2) in exudate. However, rh-bFGF had no effect on NAG content.
Conclusion: Recombinant human bFGF has an anti-inflammatory effect and its mechanisms are related to the inhibition of NOS activity, reduction of NO, MDA, and PGE(2) content, and increase of SOD activity.
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TL;DR: Pharmacological studies are continuing in order to characterize the mechanism(s) responsible for the antinociceptive and anti-inflammatory actions of caulerpin and also to identify other active principles present in Caulerpa racemosa.
Abstract: The antinociceptive and anti-inflammatory activity of caulerpin was investigated. This bisindole alkaloid was isolated from the lipoid extract of Caulerpa racemosa and its structure was identified by spectroscopic methods, including IR and NMR techniques. The pharmacological assays used were the writhing and the hot plate tests, the formalin-induced pain, the capsaicin-induced ear edema and the carrageenaninduced peritonitis. Caulerpin was given orally at a concentration of 100 μmol/kg. In the abdominal constriction test caulerpin showed reduction in the acetic acid-induced nociception at 0.0945 μmol (0.0103–1.0984) and for dypirone it was 0.0426 μmol (0.0092–0.1972). In the hot plate test in vivo the inhibition of nociception by caulerpin (100 μmol/kg, p.o.) was also favorable. This result suggests that this compound exhibits a central activity, without changing the motor activity (seen in the rotarod test). Caulerpin (100 μmol/kg, p.o.) reduced the formalin effects in both phases by 35.4% and 45.6%, respectively. The possible anti-inflammatory activity observed in the second phase in the formalin test of caulerpin (100 μmol/kg, p.o.) was confirmed on the capsaicin-induced ear edema model, where an inhibition of 55.8% was presented. Indeed, it was also observed in the carrageenan-induced peritonitis that caulerpin (100 μmol/kg, p.o.) exhibited anti-inflammatory activity, reducing significantly the number of recruit cells by 48.3%. Pharmacological studies are continuing in order to characterize the mechanism(s) responsible for the antinociceptive and anti-inflammatory actions and also to identify other active principles present in Caulerpa racemosa.
135 citations
Cites background from "Effect of recombinant human basic f..."
...It is well-known that reactive oxygen species, nitric oxide and PGE2 are considered as inflammatory factors, and play important roles in damage of tissues by inflammation [44]....
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TL;DR: Treatment with basic fibroblast growth factor was effective in restoring the morphologic and biomechanical properties of the injured superficial digital flexor tendon and could be valuable in clinical trial studies.
Abstract: The present study was designed to investigate the effects of basic fibroblast growth factor on the healing of the acute phase of complete superficial digital flexor tendon rupture in rabbits. A total of 40 skeletally mature female white New Zealand rabbits were randomly divided into 2 equal groups of injured treated and injured control. After tenotomy and surgical anastomosis, using a modified Kessler and running pattern, the injured legs were placed in casts for 14 days, and basic fibroblast growth factor was injected subcutaneously over the lesion on days 3, 7, and 10 after injury. The injured control rabbits received a normal saline injection in a similar protocol. The rabbits' weight, tendon diameter, clinical signs, radiographs, and ultrasound scans were evaluated weekly. The rabbits were killed 28 days after injury, and the tendons were evaluated at the macroscopic, histopathologic, and ultrastructural levels and for biomechanical and the percentage of dry weight analysis. Treatment significantly reduced the diameter and increased the echogenicity and dry weight content and enhanced the maturation rate of the tenoblasts, fibrillogenesis, collagen fibril diameter, fibrillar density, tensile strength, and stiffness and stress of the injured tendons. Treatment with basic fibroblast growth factor was effective in restoring the morphologic and biomechanical properties of the injured superficial digital flexor tendon and could be valuable in clinical trial studies.
50 citations
Cites background from "Effect of recombinant human basic f..."
...It has been reported that bFGF inhibits acute inflammation and pain at a therapeutic dosage by reducing the activity of prostaglandin E2, nitric oxide, scavenging oxygen free radicals, and some additional collagenolytic matrix metalloproteinases (17)....
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TL;DR: It is suggested that hydrogel impregnated with bFGF induces regeneration of the tympanic membrane and can conservatively treat tyMPanic membrane perforation.
Abstract: HypothesisIn this developmental research study that aimed to develop tympanic membrane regeneration therapy, we devised a method of sustained bFGF formulation release using gelatin hydrogel in a guinea pig eardrum perforation model.BackgroundBasic fibroblast growth factor (bFGF) can promote perforat
19 citations
Cites background from "Effect of recombinant human basic f..."
...The use of bFGF in the presence of inflammation has been shown to enhance leukocyte recruitment and endothelial cell adhesion molecule (CAM) expression during inflammation (18), which exerts an indirect antimicrobial activity in the ear (19)....
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TL;DR: The topical application of fibroblast growth factor-2 to human traumatic tympanic membranes is suggested to be safe, and otorrhoea was the most common short-term side-effect; other less common side-effects included otitis media with effusion and reperforation.
Abstract: SUMMARY The objective of this study was to investigate the short- and long-term adverse effects of fibroblast growth factor-2 treatment of tympanic membrane perforations. A total of 134 patients with traumatic tympanic membrane perforations were randomly divided into two groups: an observational group and a fibroblast growth factor-2 treatment group. The closure rate, closure time and principal side-effects were compared between the groups at 6 and 12 months. At 6 months, 131 patients were examined to determine healing outcomes and short-term side-effects. The total closure rate differed significantly between the fibroblast growth factor-2 and observational groups (95.5% vs 73.4, p #x003C; 0.01). The fibroblast growth factor-treated group exhibited a significantly shorter closure time than the observational group (11.9±3.1 days vs 52.6 ± 18.1 days, p = 0.00). Three patients with secondary otitis media with effusion, and three with reperforations, were noted in the fibroblast growth factor-2 group. We additionally performed long-term follow-up on 89.1% of the patients in the observational group and 92.5% of the patients in the fibroblast growth factor-2 group; follow-up was performed 16-42 months after perforation closure. Only a small perforation of the pars flaccida developed in the fibroblast growth factor-2 group. No middle ear cholesteatoma was noted in either group. This study suggests that the topical application of fibroblast growth factor-2 to human traumatic tympanic membranes is safe. Otorrhoea was the most common short-term side-effect; other less common side-effects included otitis media with effusion and reperforation. No serious long-term side-effects were found.
15 citations
01 Jan 2009
TL;DR: Lipozomii with SOD au fost mai eficienţi decât soluţia cu SOD in prevenirea peritonitei, iar efectele lor au fost similare in tratamentul peritonitis.
Abstract: This work describes the effect of superoxid dismutase (SOD) in the prevention and treatment of chemical induced peritonitis in rats. SOD was administered i.p., both as a solution and loaded in liposomes, in a dose of 500 U/kg body weight. SOD efficiency was compared with diclofenac, vitamin E and a nonselective nitric oxide synthase inhibitor, Nnitro L-arginine methyl ester, noted as NAME. The antiinflamatory effect was evaluated through bone marrow acute phase response and some global and specific oxidative stress parameters. SOD liposomes were more efficient than the solution of SOD in the prevention of peritonitis and their effect was similar in the treatment of peritonitis. Rezumat Aceasta lucrare prezinta efectul lipozomilor cu superoxiddismutaza (SOD) in prevenirea si tratamentul peritonitei induse chimic la sobolani. SOD a fost administrata i.p., atât ca soluţie, cât si incorporata in lipozomi, in doze de 500 U/kg corp. Eficienţa SOD a fost comparata cu cea a diclofenacului, vitaminei E si a unui inhibitor neselectiv de nitric oxid sintetaza, esterul metilic al N-nitro L-argininei, notat NAME. Efectul antiinflamator a fost evaluat prin raspunsul medular de faza acuta si prin parametrii generali si specifici de stres oxidativ. Lipozomii cu SOD au fost mai eficienţi decât soluţia cu SOD in prevenirea peritonitei, iar efectele lor au fost similare in tratamentul peritonitei.
11 citations
Cites methods from "Effect of recombinant human basic f..."
...The peritonitis was induced by the injection of a carrageenan solution (25 mg / kg body weight) [9]....
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