Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells
TL;DR: Results suggested that the proteic extract of R. communis changed the capability of binding of the red blood cells due to the stannous ion oxidation, modifying the cells structure.
Abstract: The aim of this study was to evaluate the influence of the proteic extract of R. communis on the cell physiology by the osmotic fragility, labeling of the blood elements with the 99mTc and cell morphology. To evaluate the osmotic fragility, the blood samples of the Wistar rats were incubated with the concentrations of R. communis and with the solutions of NaCl (0.4; 0.7; 0.9%). In the labeling of the blood elements procedure, the rat blood was treated with a solution of Tc-99m and TCA at 5%, determining the rate of radioactivity (%ATI) in the plasma (P) and in the red blood cells (RBC). The soluble and insoluble fractions of the plasma were also evaluated. The cells morphology submitted to the extract was evaluated by the optical microscopy (x40). The results indicated that the rate of the hemolysis increased in the presence of 0.125 mg/mL of the extract. There was a decay of 49.69% in the rate of ATI in the insoluble fraction of the cells, with the morphological alterations in the red blood cells. These results suggested that the extract changed the capability of binding of the red blood cells due to the stannous ion oxidation, modifying the cells structure.
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TL;DR: The A. occidentale extract has potential as an antimicrobial agent with low eukaryotic cell toxicity or mutagenic activity, and might as well be a safe and effective phytotherapeutic alternative.
33 citations
TL;DR: In this article, the combinations of phytocomponents from fractionated leaf extracts of Anacardium occidentale, Psidium guajava, and Terminalia catappa that stabilized sickle erythrocyte membrane against osmotic stress, or otherwise, using standard chromatographic-spectrophotometric techniques, namely GC-MS, FT-IR, and UV-visible systems.
3 citations
01 Sep 2015
TL;DR: There is antitumor activity in the NOFRC, but that it alters the biodistribution of Tc-99m, a fact which may result in failures in the diagnostic imaging process using this radioisotope as a marker.
Abstract: Ricinus communis is a plant belonging to the Euforbiaceae family. From its fruits, it is extracted the castor oil and another highly toxic fraction (non-oily fraction). In this study, we evaluated the effect of the non-oily fraction of R. communis (NOFRC) on the bio distribution of Tc-99m in healthy mice and in animals transplanted with Sarcoma-180 (S180). To evaluate the bio distribution of theTc-99m, the percentage of the total injected radioactivity (% TIR) was measured through a gamma counter. There was an increase in the capitation of Tc-99m after the treatment with NOFRC in the kidneys, brain, and stomach of healthy animals. A decrease in the reception of this radionuclide was only found in the muscles. In animals with tumour, there was a significant reduction in the uptake of Tc-99m in the heart, lungs and tumour. An increase in the capitation was only observed in the kidneys. It was also observed a reduction in the total plasma protein concentration in animals with tumours after the treatment with NOFRC, besides significant antitumor activity. We concluded that there is antitumor activity in the NOFRC, but that it alters the biodistribution of Tc-99m, a fact which may result in failures in the diagnostic imaging process using this radioisotope as a marker.
19 May 2015
TL;DR: Os eritrocitos tem sido utilizados como m odelo para avaliar a citotoxicidade de uma grande variedade of substâncias permitindo obter informacao sobre os efeitos destas sobre a membrana celular.
Abstract: s produtos naturais biologicamente ativos podem ser de origem vegetal, animal e
mineral, embora haja um
predominio de recursos
vegetais
. Estes representam uma rica
fonte de compostos biologicamente ativos e sao um exemplo de diversidade molecular
.
Amburana cearensis
e um genero de
plantas
brasile
iras
,
especi
fica
mente
da regiao
N
ordeste
conhecida po
pularmente como cumaru
-
do
-
ceara
e
amburana. As analises
quimicas e farmacologicas desta planta tem demonstrado a presenca de cumarinas como
um dos componentes quimicos mais abundante provavelmente sendo
este o responsavel,
juntamente com outras s
ubstâncias, pela acao benefica
das infusoes das cascas.
As
cumarinas sao derivad
a
s do metabolismo da fenilalanina, tendo como precursores
iniciais os acidos cinâmico e
p
-
hidroxi
-
cinâmico, a partir dos quais as cu
marinas e seus
derivados sao biossintetizados por diferentes vias.
A potencia dos antibioticos esta ameacada pela resistencia bacteriana
, aumentando
a necessidade de novos farmacos e novas classes de antibioticos
.
Os eritrocitos tem sido
utilizados como m
odelo para avaliar
a citotoxicidade
de uma grande variedade de
substâncias permitindo obter
informacao sobre os efeitos destas sobre a membrana
celular.
Dessa maneira o objetivo do trabalho foi av
a
liar
o perfil de toxicidade de
algumas cumarinas
(
1,2
-
ben
zopirona
e a
4
-
coumarinol
) foi realizada a determinacao
do
potencial citotoxico frente a eritrocitos humanos
dos grupos
A, B e O
, assim como, o
potencial citotoxico frente a
bacterias
de importância
clinica
, que
foi avaliado atraves da
determinacao da
Conc
entracao I
nibitoria
M
inima (CIM)
atraves da
tecnica de
microdiluicao.
As cumarinas de
A.
cearensis
foram capazes
de induzir hemolise em
eritrocitos humanos pertencentes aos tres tipos sanguineos do sistema ABO,
entretanto
em porcentagens muito baixas
,
nao
comprometendo
seu papel fundamental de
transporte de gases no organismo.
A
1,2
-
benzopirona
e a
4
-
coumarinol
, cumarinas de
A.
cearensis
apresentaram efeito
antimicrobiano
frente
as
linhagens Gram positivas e
Gram negativas com concentracao inibitoria minima
que variou entre
6,7
μg
e
200
μg.
Este resultado e relevante uma vez
que bacterias Gram positivas sao normalmente mais
sensiveis a antibioticos do
que as bacteria
s Gram negativas
.
A integridade
da membrana
celular depende do
tonus celular e de substâncias q
ue podem interferir na matriz da
10
membrana
, todavia as cumarinas de
A. cearensis
nao modificaram o funcionamento
da
membrana dos eritrocitos humanos
References
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Book•
01 Jan 1984
1,329 citations
Book•
17 Oct 1979
TL;DR: 1. The Atom 2. Radioactive decay 3. Instruments for radiationdetection and measurement 4. Production of radionuclides 5.Radionuclide generators 6. Radiopharmaceuticals and methods ofradiolabeling 7. Characteristics of specific radiophARMaceuticals 8.Quality control of radiopharm pharmaceuticals
Abstract: 1. The Atom 2. Radioactive decay 3. Instruments for radiationdetection and measurement 4. Production of radionuclides 5.Radionuclide generators 6. Radiopharmaceuticals and methods ofradiolabeling 7. Characteristics of specific radiopharmaceuticals 8.Quality control of radiopharmaceuticals 9. Nuclear pharmacy 10.Radiation dosimetry 11. Radiation regulations and protection 12. Invitro and In vivo nonimaging tests 13. Diagnostic uses ofradiopharmaceuticals in nuclear medicine Index
522 citations
Additional excerpts
...The technetium has been the most utilized radionuclide in the nuclear medicine because of its acceptable physical characteristics: six hours of plasma half-life and despicable environment impact (Saha, 1998)....
[...]
TL;DR: Ricin, abrin and related plant toxins have played interesting and important roles in the history of clinical medicine and biomedical research and have played important roles as experimental models to elucidate the intracellular trafficking of endocytosed proteins.
316 citations
"Effect of the extract of Ricinus co..." refers background or methods in this paper
...From its typical fruits the “ricin oil” is extracted which is used as the lubricant in the industry, as laxative, purgative and in the treatment of the terminal cancer in the folk medicine (Corrêa, 1984; Feijão, 1963; Olsnes, 2004; Souza et al., 1992)....
[...]
...Others studies show that the toxin ricin promotes an agglutination of the erythrocytes and precipitation of the serium proteins (Olsnes, 2004)....
[...]
TL;DR: Factors capable of interfering with the bioavailability of the radiobiocomplexes is worthwhile for secure diagnosis and the development of biological models to study these phenomena is highly relevant and desirable.
Abstract: Clinical images are worthwhile in Health Sciences and their analysis and correct interpretation aid the professionals,such as physicians, physiotherapists and occupational therapists, to make decisions and take subsequent therapeutic and/or rehabilitation measures. Other factors, besides the state of the disease, may interfere and affect the bioavailability of the radiopharmaceuticals (radiobiocomplexes) and the quality of the SPECT and PET images. Furthermore, the labeling of some of these radiobiocomplexes, such as plasma proteins, white blood cells and red blood cells, with 99mT, can also be modified. These factors include drugs (synthetic and natural) and dietary conditions, as well as some medical procedures (invasive or non-invasive), such as radiation therapy, surgical procedures, prostheses, cardioversion, intubation, chemoperfusion, external massage, immunotherapy, blood transfusion and hemodialysis. In conclusion, the knowledge about these factors capable of interfering with the bioavailability of the radiobiocomplexes is worthwhile for secure diagnosis. Moreover, the development of biological models to study these phenomena is highly relevant and desirable.
85 citations
"Effect of the extract of Ricinus co..." refers background in this paper
...The mechanism for the fixation of TC in the RBC consists in the binding of the radioactive element in the hemoglobin by the β channel found in the cells membrane (Bernardo-Filho et al., 2005), but the Tc labeling also depends on reduction by stannous ion....
[...]
...The mechanism for the fixation of 99mTC in the RBC consists in the binding of the radioactive element in the hemoglobin by the β channel found in the cells membrane (Bernardo-Filho et al., 2005), but the 99mTc labeling also depends on reduction by stannous ion....
[...]
TL;DR: This study shows that extracts of some medicinal plants can affect the radiolabeling of red blood cells with 99mTc using an in vitro technique.
Abstract: The influence of drugs on the labeling of red blood cells and plasma proteins with 99mTc has been reported. Any drug, which alters the labeling of the tracer, could be expected to modify the disposition of the radiopharmaceuticals. Red blood cells (RBC) labeled with technetium-99m (99mTc) are used for several evaluations in nuclear medicine. We have evaluated the effect of Thuya occidentalis, Peumus boldus and Nicotiana tabacum (tobacco) extracts on the labeling of RBC and plasma and cellular proteins with 99mTc. Blood was incubated with the drugs. Stannous chloride (SnCl2) solutions and 99mTc were added. Plasma (P) and blood cells (BC) were separated. The percentage of radioactivity (%ATI) bound to P and BC was determined. The %ATI on the plasma and cellular proteins was also evaluated by precipitation of P and BC samples with trichloroacetic acid (TCA) and isolation of soluble (SF) and insoluble (IF) fractions. The analysis of the results shows that there is a decrease in %ATI (from 97.64 to 75.89 percent) in BC with Thuya occidentalis extract. The labeling of RBC and plasma proteins can be decreased in presence of tobacco. This can be due either a direct or indirect effect (reactive oxygen species) of tobacco. The analysis of radioactivity in samples of P and BC isolated from samples of whole blood treated with Peumus boldus showed a rapid uptake of the radioactivity by blood cells in the presence of the Peumus boldus, whereas there was a slight decrease in the amount of 99mTc radioactivity in the TCA-insoluble fraction of plasma. This study shows that extracts of some medicinal plants can affect the radiolabeling of red blood cells with 99mTc using an in vitro technique.
66 citations
"Effect of the extract of Ricinus co..." refers methods in this paper
...It is used in the study of the natural or synthetic drugs influence in the labeling of the RBC and the plasma proteins (Braga et al., 2000; Dantas et al., 2005; Holanda, 2004; Oliveira et al., 2000; Souza et al., 2005)....
[...]