Journal ArticleDOI
Electrochemical detection of human brain transmitter amino acids by high-performance liquid chromatography of stable o-phthalaldehyde-sulphite derivatives.
Reads0
Chats0
TLDR
A simple, sensitive, reliable and reproducible isocratic HPLC technique for the measurement of OPA/sulphite derivatives of human brain amino acid neurotransmitters is described, applied to the determination of GABA and glutamate in brain tissue taken post-mortem from patients with Huntington's disease and control subjects.Abstract:
A simple, sensitive, reliable and reproducible isocratic HPLC technique for the measurement of OPA/sulphite derivatives of human brain amino acid neurotransmitters is described. This employs a sample preparation that is also compatible with the concurrent determination of monoamines and their metabolites on a separate HPLC system. The method has been applied to the determination of GABA and glutamate in brain tissue taken post-mortem from patients with Huntington's disease and control subjects.read more
Citations
More filters
Journal ArticleDOI
Determination of in vivo amino acid neurotransmitters by high-performance liquid chromatography with o-phthalaldehyde-sulphite derivatisation.
TL;DR: This technique has the sensitivity to determine the concentrations of amino acid neurotransmitters in cerebrospinal fluid (CSF) and an in vivo microdialysis method is discussed for the detection of basal and potassium-stimulated levels of gamma-aminobutyric acid and glutamate from rat hippocampus.
Journal ArticleDOI
Adenosine A2a Receptor‐Mediated Modulation of Striatal [3H]GABA and [3H]Acetylcholine Release
Ian P. Kirk,Peter J. Richardson +1 more
TL;DR: It is concluded that adenosine A2a receptors are present on both GABAergic and cholinergic striatal nerve terminals where they inhibit and stimulate transmitter release, respectively.
Journal ArticleDOI
Low cumulative manganese exposure affects striatal GABA but not dopamine
TL;DR: An increased environmental burden of manganese may have deleterious effects on populations with sub-threshold neurodegeneration in the basal ganglia (e.g. pre-Parkinsonism) with increasing cumulative dose, whereby GABA levels are adversely affected before striatal dopamine levels.
Journal ArticleDOI
The analysis of excitatory, inhibitory and other amino acids in rat brain microdialysates using microbore liquid chromatography
TL;DR: Three microbore liquid chromatography assays for determination of amino acids in rat brain dialysates are described, with a 5-fold increase in sensitivity for GABA and fast measurement of glutamate and aspartate.
Journal ArticleDOI
Endogenous nitric oxide facilitates striatal dopamine and glutamate efflux in vivo: role of ionotropic glutamate receptor-dependent mechanisms.
TL;DR: It is demonstrated that endogenous NO production, stimulated via H-ARG-dependent activation of type 1 NOS, enhances striatal DA efflux via an increase in glutamatergic tone on ionotropic GLU-receptors.
References
More filters
Journal ArticleDOI
Automatic recording apparatus for use in the chromatography of amino acids.
Journal ArticleDOI
Rapid analysis of amino acids using pre-column derivatization.
TL;DR: The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydrolyzates and the liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids.
Journal ArticleDOI
High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde
Peter Lindroth,Kenneth Mopper +1 more
Journal ArticleDOI
Huntington's chorea. Deficiency of gamma-aminobutyric acid in brain.
TL;DR: Deficiency of γ-aminobutyric acid, a possible inhibitory synaptic transmitter in brain, might produce the symptoms characteristic of Huntington's chorea.
Journal ArticleDOI
Amino acid analysis with fluorescamine at the picomole level
TL;DR: An amino acid analyzer based on standard column chromatographic separation techniques, but with a novel fluorometric detection system utilizing fluorescamine is described, which is two orders of magnitude more sensitive than commercial analyzers employing the colorimetric ninhydrin procedure.
Related Papers (5)
o-Phthalaldehyde—sulfite derivaization of primary amines for liquid chromatography—electrochemistry
High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde
Peter Lindroth,Kenneth Mopper +1 more