Journal ArticleDOI
Electron microscopy of human fibroblasts in tissue culture during logarithmic and confluent stages of growth.
David E. Comings,T.A. Okada +1 more
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TLDR
The most striking changes in the fibroblasts as they passed into confluency were the dilatation of the endoplasmic cisternae, the loss of the polyribosome configuration, and the increase in the number of microfilaments.About:
This article is published in Experimental Cell Research.The article was published on 1970-08-01. It has received 76 citations till now. The article focuses on the topics: Confluency & Endoplasmic reticulum.read more
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HUMAN VASCULAR ENDOTHELIAL CELLS IN CULTURE Growth and DNA Synthesis
TL;DR: Liquid scintillation measurements of [3H]thymidine incorporation in primary and secondary endothelial cultures in microwell trays showed a similar correlation of DNA synthesis with cell density, indicating that endothelial cell cultures may provide a useful in vitro model for studying pathophysiologic factors in endothelial regeneration.
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Replicative senescence: a critical review.
TL;DR: This review discusses replicative senescence in the light of current information on signal transduction and mitogenesis, cell stress, apoptosis, telomere changes and finally replicativesenescence as a model of aging in vivo.
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Ultrastructural changes accompanying the aging of human diploid cells in culture.
TL;DR: The ultrastructure of human diploid fibroblasts, strain WI-38, was studied at several different passage levels, ranging from young (15th passage) to old (56th passage), and a number of age-related ultrastructural changes were observed and quantitatively expressed.
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A comparative analysis of the cell biology of senescence and aging
TL;DR: Comparison of age-related changes and their underlying mechanisms in in vitro senescent cells and aged postmitotic cells would reveal the relevance of replicative senescence to the physiological processes occurring in postmitosis cells as individuals age.
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Association of chromatin fibers with the annuli of the nuclear membrane.
David E. Comings,T.A. Okada +1 more
TL;DR: Electron microscopy of whole mount preparations of mammalian and avian interphase nuclei indicate that the chromatin fibers converge and attach to the annuli of the nuclear membrane, and similar configurations were seen in water-spread meiotic and mitotic chromosomes.
References
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Journal ArticleDOI
The limited in vitro lifetime of human diploid cell strains
TL;DR: The survival curves obtained with human diploid cell strains are comparable to “multiple-hit” or “ multiple-target” curves obtain with other biological systems where an initial threshold dose is required before an exponential form of the curve is established.
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The fibroblast and wound repair
TL;DR: This review of connective tissue repair has attempted to place into historical perspective information obtained by newer approaches, what is known of the inflammatory response, the fine structure of the connective tissues cells in healing wounds and with correlated chemical findings in these tissues.
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An analysis of collagen secretion by established mouse fibroblast lines
Burton Goldberg,Howard Green +1 more
TL;DR: In vitro synthesis of collagen by established mouse fibroblast lines has been examined by electron microscopy and the secretion of collagen is concluded to be of the merocrine type.
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Studies on the intracellular digestive process in mammalian tissue culture cells.
TL;DR: DNA-protein coacervates containing colloidal gold particles were readily phagocytized by strain L fibroblasts and served as markers which permitted the demonstration of the evolution of digestive vacuoles to multivesicular bodies and finally to dense bodies.