Electrospun PCL/gelatin composite nanofiber structures for effective guided bone regeneration membranes.
01 Sep 2017-Materials Science and Engineering: C (Mater Sci Eng C Mater Biol Appl)-Vol. 78, pp 324-332
TL;DR: The design of electrospun polymer/protein nanofiber membranes was effective for guided bone regeneration and the in vitro osteogenesis characterizations, alizarin red in normal medium and osteogenesis medium, indicated that the nanofibers could promote bone formation.
About: This article is published in Materials Science and Engineering: C.The article was published on 2017-09-01. It has received 256 citations till now. The article focuses on the topics: Bone regeneration & Nanofiber.
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01 Jun 2010
TL;DR: The authors conclude that future research should focus on the investigation of the molecular mechanisms underlying the wound healing process following GBR application and the evaluation of the pathophysiology of the GBR healing process in the presence of systemic conditions potentially affecting the skeletal system.
Abstract: The Guided Bone Regeneration (GBR) treatment concept advocates that regeneration of osseous defects is predictably attainable via the application of occlusive membranes, which mechanically exclude non-osteogenic cell populations from the surrounding soft tissues, thereby allowing osteogenic cell populations originating from the parent bone to inhabit the osseous wound. The present review discusses the evolution of the GBR biological rationale and therapeutic concept over the last two decades. Further, an overview of the GBR research history is provided with specific focus on the evidence available on its effectiveness and predictability in promoting the regeneration of critical size cranio-maxillo-facial defects, the neo-osteogenesis potential and the reconstruction of atrophic alveolar ridges before, or in conjunction with, the placement of dental implants. The authors conclude that future research should focus on (a) the investigation of the molecular mechanisms underlying the wound healing process following GBR application; (b) the identification of site and patient related factors which impact on the effectiveness and predictability of GBR therapy and (c) the evaluation of the pathophysiology of the GBR healing process in the presence of systemic conditions potentially affecting the skeletal system.To cite this article:Retzepi M, Donos N. Guided Bone Regeneration: biological principle and therapeutic applications.Clin. Oral Impl. Res. 21, 2010; 567-576.doi: 10.1111/j.1600-0501.2010.01922.x.
391 citations
TL;DR: This review aims to critically analyse the efficacy of PCL as a biomaterial for bone scaffolds to an appreciable level due to its easy availability, cost efficacy and suitability for modification.
Abstract: Bone tissue engineering using polymer based scaffolds have been studied a lot in last decades. Considering the qualities of all the polymers desired to be used as scaffolds, Polycaprolactone (PCL) polyester apart from being biocompatible and biodegradable qualifies to an appreciable level due its easy availability, cost efficacy and suitability for modification. Its adjustable physio-chemical state, biological properties and mechanical strength renders it to withstand physical, chemical and mechanical, insults without significant loss of its properties. This review aims to critically analyse the efficacy of PCL as a biomaterial for bone scaffolds.
297 citations
TL;DR: In this paper, a review of different methods for the electrospinning of polycaprolactone (PCL) and its composites for advanced applications is presented, and the steady state conditions as well as the effect of the electro-spinning parameters on the resultant morphology of electrospun fiber are also reported.
Abstract: Polycaprolactone (PCL) is one of the most used synthetic polymers for medical applications due to its biocompatibility and slow biodegradation character. Combining the inherent properties of the PCL matrix with the characteristic of nanofibrous particles, result into promising materials that can be suitable for different applications, including the biomedical applications. The advantages of nanofibrous structures include large surface area, a small diameter of pores and a high porosity, which make them of great interest in different applications. Electrospinning, as technique, has been heavily used for the preparation of nano- and micro-sized fibers. This review discusses the different methods for the electrospinning of PCL and its composites for advanced applications. Furthermore, the steady state conditions as well as the effect of the electrospinning parameters on the resultant morphology of the electrospun fiber are also reported.
115 citations
Cites background or methods from "Electrospun PCL/gelatin composite n..."
...The addition of gelatin to PCL improved adhesion and proliferation Guided bone regeneration (Bone tissue engineering) [49]...
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...Recently, a considerable amount of research has been conducted on the preparation of different electrospun PCL/nanofiber composites in order to assess the potential biomedical applications in remedying of ailments or for tissue engineering for both in-vivo and in-vitro classifications [49]....
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TL;DR: The results indicated the PCL/chitosan/PPy nanofibrous scaffolds support the adhesion, spreading and proliferation of the PC12 cells and could serve as promising neural tissue substitutes.
109 citations
TL;DR: The obtained results from the MTT assay described that OCP particles have a positive impact on the growth of the osteoblast human G-292 cells on the scaffolds make it a great candidate for the bone tissue engineering application.
99 citations
Cites background from "Electrospun PCL/gelatin composite n..."
...Among the different used biodegradable polymers [16] for fabrication of scaffold such as polyL-lactic Acid (PLLA) [2, 16, 17], PCL [16, 18, 19], polyglycolic acid (PGA), polylactic acid (PLA) and poly(lactic-co-glycolic) acid (PLGA) [10, 16, 20], the PCL has a better mechanical strength and more compatible degradation rate with the bone proliferation [10-12]....
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References
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TL;DR: An overview of electrospinning can be found in this article, where the authors focus on progress achieved in the last three years and highlight some potential applications associated with the remarkable features of electro-spun nanofibers.
Abstract: Electrospinning provides a simple and versatile method for generating ultrathin fibers from a rich variety of materials that include polymers, composites, and ceramics. This article presents an overview of this technique, with focus on progress achieved in the last three years. After a brief description of the setups for electrospinning, we choose to concentrate on the mechanisms and theoretical models that have been developed for electrospinning, as well as the ability to control the diameter, morphology, composition, secondary structure, and spatial alignment of electrospun nanofibers. In addition, we highlight some potential applications associated with the remarkable features of electrospun nanofibers. Our discussion is concluded with some personal perspectives on the future directions in which this wonderful technique could be pursued.
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TL;DR: Two complementary strategies can be used in the fabrication of molecular biomaterials as discussed by the authors : chemical complementarity and structural compatibility, both of which confer the weak and noncovalent interactions that bind building blocks together during self-assembly.
Abstract: Two complementary strategies can be used in the fabrication of molecular biomaterials. In the 'top-down' approach, biomaterials are generated by stripping down a complex entity into its component parts (for example, paring a virus particle down to its capsid to form a viral cage). This contrasts with the 'bottom-up' approach, in which materials are assembled molecule by molecule (and in some cases even atom by atom) to produce novel supramolecular architectures. The latter approach is likely to become an integral part of nanomaterials manufacture and requires a deep understanding of individual molecular building blocks and their structures, assembly properties and dynamic behaviors. Two key elements in molecular fabrication are chemical complementarity and structural compatibility, both of which confer the weak and noncovalent interactions that bind building blocks together during self-assembly. Using natural processes as a guide, substantial advances have been achieved at the interface of nanomaterials and biology, including the fabrication of nanofiber materials for three-dimensional cell culture and tissue engineering, the assembly of peptide or protein nanotubes and helical ribbons, the creation of living microlenses, the synthesis of metal nanowires on DNA templates, the fabrication of peptide, protein and lipid scaffolds, the assembly of electronic materials by bacterial phage selection, and the use of radiofrequency to regulate molecular behaviors.
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TL;DR: This review summarizes the most recent and state of the art work in electrospinning and its uses in tissue engineering and drug delivery and its ability to fabricate fibers with diameters on the nanometer size scale.
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TL;DR: This study proposes a cost-effective composite consisting of a nanofibrous scaffold directly electrospun onto a polyurethane dressing (Tegaderm, 3M Medical) - which it is called the Tegaderm-nanofiber (TG-NF) construct - for dermal wound healing.
802 citations
TL;DR: In this article, the reaction mechanism of chitosan, bovine serum albumin (BSA), and gelatin with genipin (a natural crosslinking reagent) was examined with infrared, ultraviolet-visible, and 13C NMR spectroscopies; protein-transfer reaction mass spectrometry; photon correlation spectroscopy; and dynamic oscillatory rheometry.
Abstract: The reaction mechanism of chitosan, bovine serum albumin (BSA), and gelatin with genipin (a natural crosslinking reagent) was examined with infrared, ultraviolet–visible, and 13C NMR spectroscopies; protein-transfer reaction mass spectrometry; photon correlation spectroscopy; and dynamic oscillatory rheometry. Two reactions that proceeded at different rates led to the formation of crosslinks between primary amine groups. The fastest reaction to occur was a nucleophilic attack on genipin by a primary amine group that led to the formation of a heterocyclic compound of genipin linked to the glucosamine residue in chitosan and the basic residues in BSA and gelatin. The second, slower, reaction was the nucleophilic substitution of the ester group possessed by genipin to form a secondary amide link with chitosan, BSA, or gelatin. A decreased crosslinking rate in the presence of deuterium oxide rather than water suggested that acid catalysis was necessary for one or both of the reactions to proceed. The behavior of the gel time with polymer concentration was consistent with second-order gelation kinetics resulting from an irreversible crosslinking process, but was complicated by the oxygen radical-induced polymerization of genipin that caused the gels to assume a blue color in the presence of air. The lower elastic modulus attained after a given time during crosslinking of the globular protein BSA as compared to the coiled protein gelatin, despite possessing more crosslinkable basic residues, demonstrated the importance of protein secondary and tertiary structures in determining the availability of sites for crosslinking with genipin in protein systems. © 2003 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 41: 3941–3953, 2003
541 citations