Electrospun regenerated Antheraea pernyi silk fibroin scaffolds with improved pore size, mechanical properties and cytocompatibility using mesh collectors.
14 Jul 2021-Journal of Materials Chemistry B (The Royal Society of Chemistry)-Vol. 9, Iss: 27, pp 5514-5527
TL;DR: In this paper, the conductive metal meshes were used as collectors to prepare modified Antheraea pernyi silk fibroin (RASF) scaffolds by electrospinning from its aqueous solution.
Abstract: Generally, electrospun silk fibroin scaffolds collected by traditional plates present limited pore size and mechanical properties, which may restrict their biomedical applications. Herein, regenerated Antheraea pernyi silk fibroin (RASF) with excellent inherent cell adhesion property was chosen as a raw material and the conductive metal meshes were used as collectors to prepare modified RASF scaffolds by electrospinning from its aqueous solution. A traditional intact plate was used as a control. The morphology and mechanical properties of the obtained scaffolds were investigated. Schwann cells were further used to assess the cytocompatibility and cell migration ability of the typical scaffolds. Interestingly, compared with the traditional intact plate, the mesh collector with an appropriate gap size (circa 7 mm) could significantly improve the pore size, porosity and mechanical properties of the RASF scaffolds simultaneously. In addition, the scaffold collected under this condition (RASF-7mmG) showed higher cell viability, deeper cell permeation and faster cell migration of Schwann cells. Combined with the excellent inherent properties of ASF and the obviously enhanced scaffold cytocompatibility and mechanical properties, the RASF-7mmG scaffold is expected to be a candidate with great potential for biomedical applications.
Citations
More filters
TL;DR: In this article , a unique porous scaffold based on regenerated silk fibroin (RSF) was fabricated by using freeze-drying after enzymatical crosslinking.
Abstract: Due to the lack of blood and nutrition supply, the repairing of cartilage defect has become a challenging topic in clinical treatment. Herein, a unique porous scaffold based on regenerated silk fibroin (RSF) was fabricated by using freeze-drying after enzymatical crosslinking. Results showed that the pore size, mechanical properties and cytocompatibility of the scaffold were all significantly improved by proper combination of bacterial cellulose nanofiber ribbon (BCNR), thus much appropriate for cartilage regeneration. Based on this RSF/BCNR composite scaffold , the effects of stair-stepping shaped dynamic hydrostatic pressure (DHP) on the construction of tissue engineered cartilage in vitro and dynamic loading microenvironment on the regeneration of cartilage defect in vivo have been comprehensively investigated for the first time. Compared with traditional static culture, DHP has obviously enhancing chondrocyte growth and cartilage specific matrix deposition. Compared with the unloading microenvironment in vivo , corresponding dynamic loading microenvironment significantly improved cartilage regeneration, as it presented apparent cartilage lacuna like structure and thicker newly formed cartilage tissue. The insights of in vitro and in vivo dynamic mechanical stimulations enhancing effects on cartilage regeneration based on the RSF/BCNR composite scaffold revealed in this study could provide valuable guidance for the construction of optimal tissue engineered cartilage and the clinical prevention and treatment of orthopedic diseases. • A unique RSF/BCNR composite scaffold with improved pore size, mechanical strength and cytocompatibility was fabricated. • Effects of dynamic mechanical stimulations on the in vitro and in vivo cartilage regeneration have been comprehensively investigated. • Dynamic mechanical stimulations have significantly improved cartilage regeneration based on the RSF/BCNR scaffold.
21 citations
TL;DR: Wang et al. as mentioned in this paper describe varied building blocks of silk at different levels used in biomedical field and their effective extraction and reconstruction strategies, and present recent discoveries and research progresses on how these functional regenerated silk fibroin (RSF) biomaterials used in advanced biomedical applications, especially in the fields of cell-material interactions, soft tissue regeneration, and flexible bioelectronic devices.
Abstract: Silk fibroin has become a promising biomaterial owing to its remarkable mechanical property, biocompatibility, biodegradability, and sufficient supply. However, it is difficult to directly construct materials with other formats except for yarn, fabric and nonwoven based on natural silk. A promising bioinspired strategy is firstly extracting desired building blocks of silk, then reconstructing them into functional regenerated silk fibroin (RSF) materials with controllable formats and structures. This strategy could give it excellent processability and modifiability, thus well meet the diversified needs in biomedical applications. Recently, to engineer RSF materials with properties similar to or beyond the hierarchical structured natural silk, novel extraction and reconstruction strategies have been developed. In this review, we seek to describe varied building blocks of silk at different levels used in biomedical field and their effective extraction and reconstruction strategies. This review also present recent discoveries and research progresses on how these functional RSF biomaterials used in advanced biomedical applications, especially in the fields of cell-material interactions, soft tissue regeneration, and flexible bioelectronic devices. Finally, potential study and application for future opportunities, and current challenges for these bioinspired strategies and corresponding usage were also comprehensively discussed. In this way, it aims to provide valuable references for the design and modification of novel silk biomaterials, and further promote the high-quality-utilization of silk or other biopolymers.
17 citations
TL;DR: The ASF NFs enhanced 16G/4ASFNFs scaffold reported here are expected to be a candidate with excellent potential for biomedical applications and successfully improve the shape fidelity and porosity of the 3D printed scaffold.
14 citations
TL;DR: A highly sensitive, biocompatible, and degradable temperature sensor was proposed to detect the living cell extracellular environments and worked at promising response of cells at different temperatures.
Abstract: Temperature is one of the key parameters for activity of cells. The trade-off between sensitivity and biocompatibility of cell temperature measurement is a challenge for temperature sensor development. Herein, a highly sensitive, biocompatible, and degradable temperature sensor was proposed to detect the living cell extracellular environments. Biocompatible silk materials were applied as sensing and packing layers, which endow the device with biocompatibility, biodegradability, and flexibility. The silk-based temperature sensor presented a sensitivity of 1.75%/°C and a working range of 35-63 °C with the capability to measure the extracellular environments. At the bending state, this sensor worked at promising response of cells at different temperatures. The applications of this developed silk material-based temperature sensor include biological electronic devices for cell manipulation, cell culture, and cellular metabolism.
12 citations
11 citations
References
More filters
TL;DR: In this article, a collector consisting of two pieces of electrically conductive substrates separated by a gap whose width could be varied from hundreds of micrometers to several centimeters.
Abstract: Electrospinning has been applied to prepare uniaxially aligned nanofibers made of organic polymers, ceramics, and polymer/ceramic composites The key to the success of this method was the use of a collector consisting of two pieces of electrically conductive substrates separated by a gap whose width could be varied from hundreds of micrometers to several centimeters As driven by electrostatic interactions, the charged nanofibers were stretched to span across the gap and thus to become uniaxially aligned arrays over large areas Because the nanofibers were suspended over the gap, they could be conveniently transferred onto the surfaces of other substrates for subsequent treatments and various applications Materials that have been successfully incorporated into this procedure include conventional organic polymers, graphite carbon, and metal oxides By controlling the parameters for electrospinning, we have also fabricated a number of simple device structures, for example, an individual nanofiber spanning
1,460 citations
TL;DR: The understanding of silk chemistry, the limitations in being able to reconstitute silks and to generate them synthetically, and a range of applications that have been developed using silk materials are reviewed.
Abstract: Spiders and silkworms generate silk protein fibers that embody strength and beauty. Orb webs are fascinating feats of bioengineering in nature, displaying magnificent architectures while providing essential survival utility for spiders. The unusual combination of high strength and extensibility is a characteristic unavailable to date in synthetic materials yet is attained in nature with a relatively simple protein processed from water. This biological template suggests new directions to emulate in the pursuit of new high-performance, multifunctional materials generated with a green chemistry and processing approach. These bio-inspired and high-technology materials can lead to multifunctional material platforms that integrate with living systems for medical materials and a host of other applications.
1,272 citations
TL;DR: The findings suggest the feasibility of ME to design scaffolds with a hierarchical organization through a layer-by-layer process and control over fibre orientation and the electrospun PLA/PCL bi-layered tube presents appropriate characteristics to be considered a candidate scaffold for blood vessel tissue engineering.
460 citations
TL;DR: The pertinent work sheds new insight into the cell–material interactions, and is stimulating for biomaterial design in regenerative medicine, tissue engineering, and high‐throughput detection, diagnosis, and drug screening.
Abstract: Cell-material interactions constitute a key fundamental topic in biomaterials study. Various cell cues and matrix cues as well as soluble factors regulate cell behaviors on materials. These factors are coupled with each other as usual, and thus it is very difficult to unambiguously elucidate the role of each regulator. The recently developed material techniques of surface patterning afford unique ways to reveal the underlying science. This paper reviews the pertinent material techniques to fabricate patterns of microscale and nanoscale resolutions, and corresponding cell studies. Some issues are emphasized, such as cell localization on patterned surfaces of chemical contrast, and effects of cell shape, cell size, cell-cell contact, and seeding density on differentiation of stem cells. Material cues to regulate cell adhesion, cell differentiation and other cell events are further summed up. Effects of some physical properties, such as surface topography and matrix stiffness, on cell behaviors are also discussed; nanoscaled features of substrate surfaces to regulate cell fate are summarized as well. The pertinent work sheds new insight into the cell-material interactions, and is stimulating for biomaterial design in regenerative medicine, tissue engineering, and high-throughput detection, diagnosis, and drug screening.
418 citations
TL;DR: Aligned and random polycaprolactone/gelatin nanofibrous scaffolds were fabricated for the in vitro culture of Schwann cells that assist in directing the growth of regenerating axons in nerve tissue engineering to confirm that they are suitable substrates for Schwann cell growth as compared to PCL nanofiber scaffolds for neural tissue engineering.
271 citations