Journal ArticleDOI
Elevated recombination rates in transcriptionally active DNA.
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TLDR
Northern analysis of strains containing plasmid inserts with various promoter mutations suggests that the stimulation in recombination is mediated by events initiating within the integrated plasmID sequences.About:
This article is published in Cell.The article was published on 1989-02-24. It has received 1641 citations till now. The article focuses on the topics: Plasmid & Direct repeat.read more
Citations
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Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Frédéric Pâques,James E. Haber +1 more
TL;DR: This review encompasses different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination.
Journal ArticleDOI
The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.
TL;DR: A method that detects proteins capable of interacting with a known protein and that results in the immediate availability of the cloned genes for these interacting proteins is described and could be readily extended to mammalian proteins.
Journal ArticleDOI
Extrachromosomal rDNA circles--a cause of aging in yeast.
TL;DR: It is shown that nucleolar changes in aging yeast mother cells are likely due to the accumulation of extrachromosomal rDNA circles (ERCs) in old cells and that, in fact, ERCs cause aging.
Journal ArticleDOI
The large diverse gene family var encodes proteins involved in cytoadherence and antigenic variation of plasmodium falciparum-infected erythrocytes
Xin-zhuan Su,Virginia M. Heatwole,Samuel P. Wertheimer,Frangoise Guinet,Jacqueline A. Herrfeldt,David S. Peterson,Jeffrey A. Ravetch,Thomas E. Wellems +7 more
TL;DR: A large and extremely diverse family of P. falciparum genes (var) that encode 200-350 kDa proteins having the expected properties of antigenically variant adhesion molecules are described.
Journal ArticleDOI
On the road to cancer: aneuploidy and the mitotic checkpoint.
TL;DR: Defects in the mitotic checkpoint generate aneuploidy and might facilitate tumorigenesis, but more severe disabling of checkpoint signalling is a possible anticancer strategy.
References
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Book
Nonparametric statistics for the behavioral sciences
TL;DR: This is the revision of the classic text in the field, adding two new chapters and thoroughly updating all others as discussed by the authors, and the original structure is retained, and the book continues to serve as a combined text/reference.
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.