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Journal ArticleDOI

Elucidation of extracellular matrix mechanics from muscle fibers and fiber bundles.

24 Feb 2011-Journal of Biomechanics (Elsevier)-Vol. 44, Iss: 4, pp 771-773
TL;DR: A new method to quantify viscoelastic ECM modulus is presented by combining tests of single muscle fibers and fiber bundles, which demonstrate that ECM is a highly nonlinearly elastic material, while muscle fibers are linearly elastic.
About: This article is published in Journal of Biomechanics.The article was published on 2011-02-24 and is currently open access. It has received 156 citations till now. The article focuses on the topics: Skeletal muscle.
Citations
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Posted ContentDOI
29 Nov 2019-bioRxiv
TL;DR: Depending on the neuromechanical conditions, the muscle spindle model output appears to “encode” aspects of muscle force, yank, length, stiffness, velocity, and/or acceleration, providing an extendable, multiscale, biophysical framework for understanding and predicting proprioceptive sensory signals in health and disease.
Abstract: Sensory information about the body’s mechanical interactions with the environment are critical for neural control of movement. Muscle spindle sensory neurons richly innervate muscles in vertebrates; their firing patterns as muscles stretch have been well-characterized experimentally, but have not been fully explained mechanistically. Here, we show that a diverse range of muscle spindle firing characteristics are emergent from first principles of muscle contractile mechanics. We develop a mechanistic muscle spindle model that predicts well-known phenomena such as variations in muscle spindle sensitivity due to prior movement history and nonlinear scaling with muscle stretch velocity. The model further predicts how central commands to muscle spindles–fusimotor drive–alters their firing responses, and shows how seemingly paradoxical muscle spindle firing during voluntary force production in humans can arise. Our multiscale muscle spindle model provides a unifying biophysical framework that may broadly explain and predict movement-related sensory signals in health and disease.

2 citations


Cites background from "Elucidation of extracellular matrix..."

  • ...…spindle IFRs (Blum et al., 2019), and that only a portion of the total musculotendon force can be attributed to extrafusal muscle fiber force, with the remaining nonlinear elastic component attributed to extracellular tissues (Blum et al., 2019; G. A. Meyer & Lieber, 2011; G. Meyer & Lieber, 2018)....

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Dissertation
01 Jan 2019
TL;DR: In this paper, the authors propose a method to solve the problem of "uniformity" and "uncertainty" in the context of video games.2.3.2
Abstract: 2

2 citations

Posted ContentDOI
16 Jul 2020-medRxiv
TL;DR: Data supports the progression toward clinical application of shear wave ultrasound elastography in evaluating altered muscle mechanical properties in stroke stipulating the confirmation of muscle quiescence and will likely augment the conventional method of manually testing joint mobility.
Abstract: Muscle tissue is prone to changes in composition and architecture following stroke. Changes in muscle tissue of the extremities are thought to increase passive muscle stiffness and joint impedance. These effects likely compound neuromuscular impairments exacerbating movement function. Unfortunately, conventional rehabilitation is devoid of quantitative measures yielding to subjective assessment of passive joint mobility and end feel. Shear wave ultrasound elastography is a conventional tool used by ultrasonographers that may be readily available for use in the rehabilitation setting as a quantitative measure, albeit at the muscle-tissue level, filling the gap. To support this postulation, we evaluated the criterion validity of shear wave ultrasound elastography of the biceps brachii by investigating the relationship to a laboratory-based criterion measure for quantifying elbow joint impedance in individuals with moderate to severe chronic stroke. Measurements were performed under passive conditions at seven positions spanning the arc of elbow joint extension in both arms of twelve individuals with hemiparetic stroke. Surface electromyography was utilized for threshold-based confirmation of muscle quiescence. A significant moderate relationship was identified near end range of elbow extension and all metrics were greater in the paretic arm. Data supports the progression toward clinical application of shear wave ultrasound elastography in evaluating altered muscle mechanical properties in stroke stipulating the confirmation of muscle quiescence. Considering the lack of bedside robotics in clinical practice, shear wave ultrasound elastography will likely augment the conventional method of manually testing joint mobility. Tissue-level measurement may also assist in identifying new therapeutic targets for patient-specific impairment-based interventions.

1 citations


Cites background from "Elucidation of extracellular matrix..."

  • ...changes in the expression of titin (48) and collagen (53)....

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Journal ArticleDOI
TL;DR: The aim of this paper is to derive an inverse model based on the microstructure of a skeletal muscle that can predict its passive mechanical response and show a close match for both predicted stress-strain response for fibre and cross-fibre direction deformations and similar Poisson's ratio values.

1 citations

01 Jan 2016
TL;DR: The challenges currently facing the skeletal muscle tissue engineering are presented along with potential approaches to further the field and deliver effective treatment options for patients and their physicians.
Abstract: Volumetric muscle loss (VML) is an injury to skeletal muscle characterized by a loss of more than 20% of a muscles volume. The combination of the bulk loss of tissue, transection and separation of myofibers proximal and distal to the injury, loss of innervation and blood supply, and the depletion of muscle progenitor cells results in permanent fibrosis and functional deficits due to loss of contractile tissue. Scaffolds, cells, and engineered constructs have been explored as potential therapeutic interventions to induce myogenesis at the site of a VML injury in animal models, in addition to limited clinical trials. This dissertation summarizes the current state of the field and explores possible strategies for repairing VML and understanding the mechanisms underlying the regenerative response of VML-damaged muscle. The challenges currently facing the skeletal muscle tissue engineering are presented along with potential approaches to further the field and deliver effective treatment options for patients and their physicians. Acknowledgements I would like to thank my peers in the Regenerative Biomaterials Lab at the University of Arkansas, especially Abby Terlouw and John Kim for their assistance and collaboration in this research. Special thanks is also due to my dissertation committee: Dr. Muldoon, Dr. Washington, and Dr. Jensen for their time and feedback. And my sincerest thank you to Dr. Wolchok, for providing the opportunity and funding sources (NIH Award# R15AR064481 and Arkansas Biosciences Institute) that made all of this work possible. Dedication I would like to dedicate this paper to my wife, Faith, for her encouragement, patience, and resolute love.

1 citations

References
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Journal ArticleDOI
25 Aug 2006-Cell
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.

12,204 citations


"Elucidation of extracellular matrix..." refers background in this paper

  • ...Since substrate biomechanical properties have been shown to be critical in the biology of tissue development and remodeling (Engler et al., 2006; Gilbert et al., 2010), it is likely that mechanics are critical for ECM to perform its function....

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Journal ArticleDOI
27 Aug 2010-Science
TL;DR: Using a bioengineered substrate to recapitulate key biophysical and biochemical niche features in conjunction with a highly automated single-cell tracking algorithm, it is shown that substrate elasticity is a potent regulator of MuSC fate in culture.
Abstract: Stem cells that naturally reside in adult tissues, such as muscle stem cells (MuSCs), exhibit robust regenerative capacity in vivo that is rapidly lost in culture. Using a bioengineered substrate to recapitulate key biophysical and biochemical niche features in conjunction with a highly automated single-cell tracking algorithm, we show that substrate elasticity is a potent regulator of MuSC fate in culture. Unlike MuSCs on rigid plastic dishes (approximately 10(6) kilopascals), MuSCs cultured on soft hydrogel substrates that mimic the elasticity of muscle (12 kilopascals) self-renew in vitro and contribute extensively to muscle regeneration when subsequently transplanted into mice and assayed histologically and quantitatively by noninvasive bioluminescence imaging. Our studies provide novel evidence that by recapitulating physiological tissue rigidity, propagation of adult muscle stem cells is possible, enabling future cell-based therapies for muscle-wasting diseases.

1,428 citations


"Elucidation of extracellular matrix..." refers background in this paper

  • ...Since substrate biomechanical properties have been shown to be critical in the biology of tissue development and remodeling (Engler et al., 2006; Gilbert et al., 2010), it is likely that mechanics are critical for ECM to perform its function....

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Journal ArticleDOI
01 Jun 2009-Bone

1,224 citations


"Elucidation of extracellular matrix..." refers background in this paper

  • ...Since substrate biomechanical properties have been shown to be critical in the biology of tissue development and remodeling (Engler et al., 2006; Gilbert et al., 2010), it is likely that mechanics are critical for ECM to perform its function....

    [...]

Journal ArticleDOI
TL;DR: Avian integrin shows little specificity and appears to behave as a multifunctional, promiscuous receptor for extracellular matrix molecules, and post-translational modifications provide yet another mechanism for regulating integrin-ligand binding.
Abstract: Table 2 lists most of the extracellular matrix and related receptors identified to date. The wide range of binding affinities of these receptors for their ligands may be important to their function. The affinity of integrins for fibronectin is moderate, with a dissociation constant in the micromolar range. This affinity level leads to relatively rapid dissociation and reformation of receptor-ligand complexes. Thus changes in component concentration can shift binding equilibria within minutes (the time scale of many biologic phenomena) and change the number or organization of adhesive complexes. This type of interaction would be useful in motile cells, in which adhesions must form and dissociate rapidly. In contrast, the affinity of the 68-kDa laminin receptor for its ligand is three orders of magnitude higher. Such levels of affinity would be useful in stabilizing tissue. Members of the integrin family appear to recognize an RGD sequence on the ligands to which they bind. Since there are many ligands containing the RGD sequence, the question of specificity arises. Avian integrin shows little specificity and appears to behave as a multifunctional, promiscuous receptor for extracellular matrix molecules. Figure 1 summarizes our current view of the structural and functional features of avian integrin. In contrast, the mammalian receptors for vitronectin and fibronectin are specific for their respective ligands. More than one of these receptors may be present simultaneously on a cell surface, e.g. fibroblasts express receptors for fibronectin, laminin, and vitronectin at the same time. This multiplicity of receptors provides potential mechanisms for generating the adhesive differences among cells believed to play a prominent role in morphogenesis. Further adhesive differences may stem from the formation of different combinations of various alpha- and beta-subunits expressed in the cell. The mechanism of regulation of adhesive interactions with the extracellular matrix is only beginning to be explored. There are several levels at which this regulation might occur. Integrin appears to be more regionalized in more developed cells that are integral parts of tissue structures. Changes in receptor distribution could alter the relative strength of adhesive interactions. In certain instances, avian integrin disappears, or its concentration is reduced, e.g. during the development of embryonic lung (Chen et al 1986) and erythroid cells (Patel & Lodish 1985). Post-translational modifications provide yet another mechanism for regulating integrin-ligand binding.(ABSTRACT TRUNCATED AT 400 WORDS)

784 citations


"Elucidation of extracellular matrix..." refers background in this paper

  • ...Extracellular matrix (ECM) is essential for the development, maintenance and regeneration of skeletal muscle (Buck and Horwitz, 1987; Purslow, 2002)....

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  • ...The importance of the extracellular matrix (ECM) in muscle is widely recognized, since ECM plays a central role in proper muscle development (Buck and Horwitz, 1987), tissue structural support (Purslow, 2002), and transmission ofmechanical signals between fibers and tendon (Huijing, 1999)....

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  • ...…r a c t The importance of the extracellular matrix (ECM) in muscle is widely recognized, since ECM plays a central role in proper muscle development (Buck and Horwitz, 1987), tissue structural support (Purslow, 2002), and transmission ofmechanical signals between fibers and tendon (Huijing, 1999)....

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Journal ArticleDOI
TL;DR: The passive tension-sarcomere length relation of rat cardiac muscle was investigated by studying passive (or not activated) single myocytes and trabeculae and the contribution of collagen, titin, microtubules, and intermediate filaments to tension and stiffness was investigated.

587 citations


"Elucidation of extracellular matrix..." refers methods in this paper

  • ...Previous studies used methods of subtraction, where the ECM was ‘‘preferentially’’ digested from muscle and its properties inferred from subtracting the digested state from the undigested state (see review by Fomovsky et al., 2010; Granzier and Irving, 1995)....

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