Endopeptidase penicillin-binding proteins 4 and 7 play auxiliary roles in determining uniform morphology of Escherichia coli
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1,104 citations
Cites background from "Endopeptidase penicillin-binding pr..."
...24 [9] Barrett DS, Chen L, Litterman NK & Walker S (2004) Expression and characterization of the 25 isolated glycosyltransferase module of Escherichia coli PBP1b....
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...It has been suggested that both observed apoenzyme 22 structures may illustrate a conformational sampling of the ‘‘open’’ and ‘‘closed’’ forms that may 23 play a regulatory role in PBP1b catalytic activity....
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...E. coli 21 possesses three class A PBPs. PBP1a and PBP1b are the major transpeptidases-transglycosylases....
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...derivative of PBP1b (82-300) was also produced in the absence of detergent....
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...For example, the fragments M46-D478 and M46-Q423 retain 23% 13 and 13% of the entire PBP1b activity respectively (Terrak, et al., 1999)....
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867 citations
Cites background from "Endopeptidase penicillin-binding pr..."
...Cell shape is affected more and more as additional PBPs are removed (210, 229, 230), and the efficiency of biofilm formation decreases in step with increasingly aberrant shapes (86)....
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...Deleting a single low-molecular-weight penicillin binding protein, PBP 5, causes cells to grow with nonuniform shape (210), which is enough to interfere with biofilm formation (86)....
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785 citations
Cites background from "Endopeptidase penicillin-binding pr..."
...Type-4 PBPs are generally considered as being indirectly involved in cell morphology (Meberg et al., 2004), in daughter cell separation (Priyadarshini et al....
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...Type-4 PBPs are generally considered as being indirectly involved in cell morphology (Meberg et al., 2004), in daughter cell separation (Priyadarshini et al., 2006) and could be implicated in biofilm formation (Gallant et al., 2005) (see ‘Physiological functions of extracytoplasmic peptidoglycan…...
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609 citations
Cites background or result from "Endopeptidase penicillin-binding pr..."
...PBP5 is the major carboxypeptidase but is not specifically associated with cell division....
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...H, and -4b; and PBP5 (169, 171) (Table 1)....
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...However, a mutant with a knockout of the B. subtilis major carboxypeptidase, PBP5, displays normal morphology during exponential growth, although cells become progressively shorter after exponential growth (185)....
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...LMW carboxypeptidase dacA (20) PBP5 Control of cell shape (117, 129, 130) dacC (20) PBP6 dacD (7) PBP6b...
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...In E. coli strains defective for PBP5, a high frequency of branching is observed....
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496 citations
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Additional excerpts
...of the arabinose promoter of pBAD18 (11)....
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"Endopeptidase penicillin-binding pr..." refers methods in this paper
...The amplified PCR products were transferred by electroporation into E. coli KM-32 (21), where they were incorporated into the chromosome via homologous recombination mediated by the phage lambda recombination system as described previously (18, 21, 22)....
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...KM-32 (21), where they were incorporated into the chromosome via homologous...
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479 citations
"Endopeptidase penicillin-binding pr..." refers background in this paper
...The first is governed by a group of cytoskeleton proteins, including FtsZ, MreB, and Mbl, which polymerize as filamentous rings or helices on the inner face of the cytoplasmic membrane (3, 9, 15)....
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