Engineered whole cut meat-like tissue by the assembly of cell fibers using tendon-gel integrated bioprinting
Dong-Hee Kang,Fiona Louis,Hao Liu,Hiroshi Shimoda,Yasutaka Nishiyama,Hajime Nozawa,Makoto Kakitani,Takagi Daisuke,Daijiro Kasa,Eiji Nagamori,Shinji Irie,Shiro Kitano,Michiya Matsusaki +12 more
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In this article, tendon-gel integrated bioprinting was used to construct tendon-like gels for the fabrication of steak-like cultured meats, including muscle, fat, and vessel.Abstract:
With the current interest in cultured meat, mammalian cell-based meat has mostly been unstructured. There is thus still a high demand for artificial steak-like meat. We demonstrate in vitro construction of engineered steak-like tissue assembled of three types of bovine cell fibers (muscle, fat, and vessel). Because actual meat is an aligned assembly of the fibers connected to the tendon for the actions of contraction and relaxation, tendon-gel integrated bioprinting was developed to construct tendon-like gels. In this study, a total of 72 fibers comprising 42 muscles, 28 adipose tissues, and 2 blood capillaries were constructed by tendon-gel integrated bioprinting and manually assembled to fabricate steak-like meat with a diameter of 5 mm and a length of 10 mm inspired by a meat cut. The developed tendon-gel integrated bioprinting here could be a promising technology for the fabrication of the desired types of steak-like cultured meats. Mammalian cell-based cultured meat has mostly been unstructured, leaving a demand for artificial steak-like meat. Here the authors present an assembled steak-like tissue of bovine skeletal muscle, adipose tissue, and blood capillary tissue fabricated by tendon-gel integrated printing technology.read more
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Scaffolding Biomaterials for 3D Cultivated Meat: Prospects and Challenges.
Claire Bomkamp,Stacey C. Skaalure,Gonçalo F. Fernando,Tom Ben-Arye,Elliot W. Swartz,Elizabeth A. Specht +5 more
TL;DR: In this paper, the authors discuss the properties of vertebrate skeletal muscle that will need to be replicated in a successful product and the current state of scaffolding innovation within the cultivated meat industry.
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3D-printable plant protein-enriched scaffolds for cultivated meat development.
TL;DR: In this article , two plant-protein-enriched scaffolding compositions were evaluated as 3D-printable platforms for bovine satellite cells (BSC) maturation.
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Dual encapsulation of β-carotene by β-cyclodextrin and chitosan for 3D printing application
TL;DR: In this article , a dual encapsulation of β-carotene (CAT) by β-cyclodextrin (CCLD) and chitosan (CS) are prepared via self-assembly process by special addition order and concentration.
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Strategies to improve meat-like properties of meat analogs meeting consumers' expectations.
TL;DR: In this article , the typical sensory characteristics of animal meat products from texture, flavor, color aspects, and sensory perception during oral processing are discussed, and related strategies were detailed to improve meat-like sensory properties for meat analogs.
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Bioprocessing technology of muscle stem cells: implications for cultured meat
TL;DR: Recently, considerable progress has been made in bioprocessing technologies for muscle stem cells (MuSCs), including isolation, expansion, differentiation, and tissue building as discussed by the authors , and their applicability to refining the production process for cultured meat and accelerating its industrialization.
References
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3D bioprinting of collagen to rebuild components of the human heart
Andrew Lee,Andrew Hudson,Daniel J. Shiwarski,Joshua W. Tashman,Thomas J. Hinton,Saigopalakrishna S. Yerneni,Jacqueline M. Bliley,Phil G. Campbell,Adam W. Feinberg +8 more
TL;DR: 3D-bioprinted hearts accurately reproduce patient-specific anatomical structure as determined by micro–computed tomography and showed synchronized contractions, directional action potential propagation, and wall thickening up to 14% during peak systole.
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Metre-long cell-laden microfibres exhibit tissue morphologies and functions
Hiroaki Onoe,Teru Okitsu,Akane Itou,Midori Kato-Negishi,Riho Gojo,Daisuke Kiriya,Koji Sato,Shigenori Miura,Shintaroh Iwanaga,Kaori Kuribayashi-Shigetomi,Yukiko T. Matsunaga,Yuto Shimoyama,Shoji Takeuchi +12 more
TL;DR: Fibres encapsulating primary pancreatic islet cells and transplanted through a microcatheter into the subrenal capsular space of diabetic mice normalized blood glucose concentrations for about two weeks and may find use as templates for the reconstruction of fibre-shaped functional tissues that mimic muscle fibres, blood vessels or nerve networks in vivo.
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3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts
TL;DR: A simple approach to 3D‐print thick, vascularized, and perfusable cardiac patches that completely match the immunological, cellular, biochemical, and anatomical properties of the patient is reported and cellularized human hearts with a natural architecture are printed.
Journal ArticleDOI
Adipose-derived stem cell: a better stem cell than BMSC
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