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Journal ArticleDOI

Enhanced forskolin production in genetically transformed cultures of Coleus forskohlii by casein hydrolysate and studies on growth and organisation

Swapna Mukherjee, +2 more
- 01 Jan 2000 - 
- Vol. 22, Iss: 2, pp 133-136
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TLDR
Casein hydrolysate at 2.0 g l−1 significantly enhanced forskolin content in a rhizogenic tumourous line, GCO-RCH-2 of Coleus forskohlii of C. forskohlii and in rooty teratoma line, RC-ST-2/4, forSkolin content enhanced to 1.7 mg g−1 cell dry wt.
Abstract
Casein hydrolysate at 2.0 g l−1 significantly enhanced forskolin content (2.3 mg g−1 cell dry wt) in a rhizogenic tumourous line, GCO-RCH-2 of Coleus forskohlii. In rooty teratoma line, RC-ST-2/4, forskolin content enhanced to 1.7 mg g−1 cell dry wt in presence of 2.5 g l−1 casein hydrolysate. Unlike untransformed calli and rhizogenic/root cultures, all the forskolin yielding transformed cultures of C. forskohlii have been maintained for over 5 years.

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Citations
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Aluminium chloride enhances colchicine production in root cultures of Gloriosa superba.

TL;DR: Root cultures of Gloriosa superba were treated with 5 mm methyl jasmonate and 125 μm AlCl3 which enhanced the intracellular colchicine content of the roots by 50-fold and 63-fold, respectively and casein hydrolysate, yeast extract and silver nitrate had no significant effect on growth and colchichine accumulation in root cultures.
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Review: Colchicine, current advances and future prospects

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Embryogenic callus culture of Tribulus terrestris L. a potential source of harmaline, harmine and diosgenin

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Enhanced biosynthesis of colchicine and thiocolchicoside contents in cell suspension cultures of Gloriosa superba L. exposed to ethylene inhibitor and elicitors

TL;DR: Results clearly show that the elicitation experiment could enhance the biosynthesis of colchicine and thiocolchicoside contents level in cell suspension cultures of G. superba and the present protocol may be used for commercial supply of these important secondary metabolites in the future for therapeutic applications.
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Biotechnological approaches for the production of forskolin, withanolides, colchicine and tylophorine

TL;DR: The world market formedicinal and aromatic plants (MAPs) is huge; India is a major exporter of raw MAPsand processed plant-based drugs (Lambert et al., 1997);75% of the total exports from India are sent to sixcountries, namely France, Germany, Japan, Switzerland, the UK and the USA.
References
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Journal ArticleDOI

A revised medium for rapid growth and bio assays with tobacco tissue cultures

TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI

Nutrient requirements of suspension cultures of soybean root cells.

TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI

Establishment of forskolin yielding transformed cell suspension cultures of Coleus forskohlii as controlled by different factors

TL;DR: Suspension cultures derived from gall calli which were obtained following infection with Agrobacterium tumefaciens were established in Coleus forskohlii and showed continuous and stable production of forskolin.
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