Journal ArticleDOI
Enzymatic reactions on thin-layer chromatographic plates. II. Phospholipase A2 hydrolysis of phosphatidylcholine and separation of the products on a single plate.
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TLDR
The acyl group distributions in the 1- and 2-positions of hen egg phosphatidylcholine obtained from the gas-liquid chromatographic analysis of the methyl ester corresponding to the lyso and free fatty acid band agreed with those obtained by the method of Wells and Hanahan.About:
This article is published in Journal of Chromatography A.The article was published on 1979-05-21. It has received 9 citations till now. The article focuses on the topics: Phosphatidylcholine.read more
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Journal ArticleDOI
Focal phospholipases A2 group III injections induce cervical white matter injury and functional deficits with delayed recovery concomitant with Schwann cell remyelination.
TL;DR: The results indicate that sPLA(2)-III can create white matter pathologies that are remyelinated by Schwann cells 2 to 3 weeks after injury, and is the first report of a reaching task being able to discriminate between various grades of cervical white matter damage and varying extents of recovery.
Journal ArticleDOI
Enzymatic reactions on thin-layer chromatographic plates : I. Lipolysis of triglycerides and separation of products on a single plate
TL;DR: In this paper, a method for the lipase deacylation of triglycerides is described in which 0.1 − 0.2 ml of the enzyme solution in 1 M tris buffer (pH 8.2) containing 2 − 4 mg of protein is applied as a band on a 0.5mm thick silica gel thin-layer chromatographic plate.
Journal ArticleDOI
Stereospecific analysis of triglycerides of Glycine max, Glycine soya, Avena sativa and Avena sterilis strains.
W. P. Pan,E. G. Hammond +1 more
TL;DR: A rapid method for the stereospecific analysis of triglycerides based on enzymatic hydrolysis on thin layer plates was applied to a number of GlyCine max, Glycine soya, Avena sativa and Avena sterilis strains and large deviations from the common triglyceride pattern were not found.
Journal ArticleDOI
Evidence for diplasmalogen as the major component of rabbit sperm phosphatidylethanolamine.
TL;DR: The PE component of rabbit sperm phospholipids appears to differ from that of the other cells in having the previously unreported diplasmalogen as its major constituent.
Journal ArticleDOI
Microwave roasting and positional distribution of fatty acids of phospholipids in soybeans (Glycine max L.)
Hiromi Yoshida,Sachiko Takagi +1 more
TL;DR: In this paper, the effects of microwave roasting on the composition and positional distribution of the fatty acids were studied not only for phospholipid composition but also for positional distribution.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Isolation and chemical characterization of phosphatidyl glycerol from spinach leaves.
E. Haverkate,L.L.M. Van Deenen +1 more
TL;DR: Fatty acid analysis of several lipid fractions showed that the Δ3-trans-hexadecenoic acid, present in the leaves, is concentrated almost exclusively in phosphatidyl glycerol, and degradation experiments with phospholipase A showed that this acid is located preferentially at the 2-ester position.
Journal ArticleDOI
Molecular species of lecithins from various animal tissues.
TL;DR: The composition of lecithins from lung and brain showed many similarities irrespective of animal species, which may indicate a certain degree of tissue specificity.
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