Enzymatic reactions on thin-layer chromatographic plates. II. Phospholipase A2 hydrolysis of phosphatidylcholine and separation of the products on a single plate.
TL;DR: The acyl group distributions in the 1- and 2-positions of hen egg phosphatidylcholine obtained from the gas-liquid chromatographic analysis of the methyl ester corresponding to the lyso and free fatty acid band agreed with those obtained by the method of Wells and Hanahan.
About: This article is published in Journal of Chromatography A.The article was published on 1979-05-21. It has received 9 citations till now. The article focuses on the topics: Phosphatidylcholine.
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TL;DR: The results indicate that sPLA(2)-III can create white matter pathologies that are remyelinated by Schwann cells 2 to 3 weeks after injury, and is the first report of a reaching task being able to discriminate between various grades of cervical white matter damage and varying extents of recovery.
38 citations
TL;DR: In this paper, a method for the lipase deacylation of triglycerides is described in which 0.1 − 0.2 ml of the enzyme solution in 1 M tris buffer (pH 8.2) containing 2 − 4 mg of protein is applied as a band on a 0.5mm thick silica gel thin-layer chromatographic plate.
23 citations
TL;DR: A rapid method for the stereospecific analysis of triglycerides based on enzymatic hydrolysis on thin layer plates was applied to a number of GlyCine max, Glycine soya, Avena sativa and Avena sterilis strains and large deviations from the common triglyceride pattern were not found.
Abstract: A rapid method for the stereospecific analysis of triglycerides based on enzymatic hydrolysis on thin layer plates was applied to a number ofGlycine max, Glycine soya, Avena sativa andAvena sterilis strains. The percentage of each fatty acid on thesn-1,sn-2- andsn-3-positions was linearly related to the total percentage of the fatty acid in the triglyceride. Large deviations from the common triglyceride pattern were not found.
20 citations
TL;DR: The PE component of rabbit sperm phospholipids appears to differ from that of the other cells in having the previously unreported diplasmalogen as its major constituent.
Abstract: The question of whether diplasmalogens [1,2-di(O-1′-alkenyl) phosphatidyl derivatives] make up part of the plasmalogen component of cell phospholipids was examined using rabbit epididymal spermatozoa. These cells are readily obtained as a highly homogeneous suspension and long have been known to have high plasmalogen content. Phospholipids were determined by thin layer chromatography (TLC) with CuSO4 staining. Plasmalogens were determined by hydrolysis of the phospholipids with TCA/HCl, followed by TLC and CuSO4 staining. Ethanolamine derivatives were determined by ninhydrin. The phosphatidylethanolamine (PE) content of these cells was 29±2 μg/108 cells, 90% of which was assayed as diplasmalogen and 10% as diacyl PE. No monoplasmalogen could be detected. The presence of diplasmalogen as the major component of PE was given further support from infrared and proton nuclear magnetic resonance (1H-NMR) spectroscopy, which showed the presence of O-1′-alkenyl substituents but near absence of O-acyl substituents. The phosphatidylcholine (PC) content of the cells was 104±5 μ/108 cells, of which 50% was monoplasmalogen with the 1′-alkenyl group on the 2 position of the glycerol moiety. No diplasmalogen was found in PC. The other phospholipids in rabbit sperm were phosphatidylglycerol (PG), cardiolipin (CL), sphingomyelin (SP) and lysophosphatidylcholine (LPC). Phosphatidylserine (PS) and phosphatidylinositol (PI) were present at the limits of detectability of the TLC method. None of these phospholipids contained plasmalogen. The PE component of rabbit sperm phospholipids appears to differ from that of the other cells in having the previously unreported diplasmalogen as its major constituent.
19 citations
TL;DR: In this paper, the effects of microwave roasting on the composition and positional distribution of the fatty acids were studied not only for phospholipid composition but also for positional distribution.
Abstract: Whole soybeans were exposed to microwave roasting for 6, 12, and 20 min at a frequency of 2,450 MHz and were studied not only for phospholipid composition but also for positional distribution of the fatty acids. During microwave roasting, the greatest rate of phospholipid losses (P<0.05) was observed in phosphatidylethanolamine (PE), followed by phosphatidylcholine (PC) and phosphatidylinositol (PI), respectively. Therefore, the effects of microwave roasting on the composition and positional distribution of the fatty acids are likely clearer in PE than in PC or PI. However, the principal characteristics for the positional distribution of fatty acids are still retained during microwave roasting: unsaturated fatty acids, especially linoleic, are predominantly concentrated in the 2-position, and saturated fatty acids, especially palmitic, primarily occupy the 1-position after 12 or 20 min of roasting. The results suggest that unsaturated fatty acids located in the 2-position are significantly protected from microwave roasting.
15 citations
Cites methods from "Enzymatic reactions on thin-layer c..."
...The positional distribution of fatty acids in each of the PE, PC, and PI samples isolated by preparative TLC was determined with phospholipase A 2 hydrolysis on a silica gel plate by a modification of the method of Dutta et al. ( 20 )....
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TL;DR: In this paper, two specific and sensitive methods have been developed for the detection of primary catecholamines, their 3-O-methyl derivatives and other related phenylethylamines, based upon the unique fluorescence produced by the treatment of their fluorescamine derivatives with perchloric acid (PCA).
20 citations
TL;DR: Young albino rats were fed choline-deficient and Choline-supplemented diets, fat-free and containing two different levels of fat (4%, 30%), for 2 weeks, revealing the fatty acid pattern of the major lipid pattern.
Abstract: Young albino rats were fed choline-deficient and choline-supplemented diets, fat-free and containing two different levels of fat (4%, 30%), for 2 weeks. The fatty acid pattern of the major lipid fr...
7 citations