Journal ArticleDOI
Escherichia coli as a platform microbial host for systems metabolic engineering
Dongsoo Yang,Cindy Pricilia Surya Prabowo,Hyunmin Eun,Seon Young Park,In Jin Cho,Song Jiao,Sang Yup Lee +6 more
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In this article, the authors review the tools and strategies employed for systems metabolic engineering of E. coli and present representative examples and strategies for the production of chemicals including bio-fuels, bulk and specialty chemicals, and natural products.Abstract:
Bio-based production of industrially important chemicals and materials from non-edible and renewable biomass has become increasingly important to resolve the urgent worldwide issues including climate change. Also, bio-based production, instead of chemical synthesis, of food ingredients and natural products has gained ever increasing interest for health benefits. Systems metabolic engineering allows more efficient development of microbial cell factories capable of sustainable, green, and human-friendly production of diverse chemicals and materials. Escherichia coli is unarguably the most widely employed host strain for the bio-based production of chemicals and materials. In the present paper, we review the tools and strategies employed for systems metabolic engineering of E. coli. Next, representative examples and strategies for the production of chemicals including biofuels, bulk and specialty chemicals, and natural products are discussed, followed by discussion on materials including polyhydroxyalkanoates (PHAs), proteins, and nanomaterials. Lastly, future perspectives and challenges remaining for systems metabolic engineering of E. coli are discussed.read more
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Adaptive laboratory evolution for improved tolerance of isobutyl acetate in Escherichia coli.
Morgan M. Matson,gdPjEVujsQ,Mateo M. Cepeda,Angela Zhang,Anna E. Case,Erol S. Kavvas,Xiaokang Wang,Austin L Carroll,Ilias Tagkopoulos,Shota Atsumi +9 more
TL;DR: In this paper, adaptive laboratory evolution (ALE) was applied to E. coli for improved IBA tolerance, and 22 IBA-tolerant mutants were isolated over 37 rounds of selective pressure, these mutants not only tolerate high IBA concentrations, they also produce higher IBA titers.
Journal ArticleDOI
CRISPR/Cas Technologies and Their Applications in Escherichia coli.
Huina Dong,Yali Cui,Dawei Zhang +2 more
TL;DR: The most widely used CRISPR/Cas tools and their applications in Escherichia coli are summarized in this article, where the state-of-the-art of transcriptional regulation using CRISpri is briefly reviewed.
Journal ArticleDOI
Recent advances in microbial co-culture for production of value-added compounds
TL;DR: In this paper , the authors summarized the recent advanced progress in applying microbial platform co-culture to produce natural products, such as flavonoid, terpenoid, alkaloid, etc., and importantly different strategies for enhancing production, overcoming the metabolic burdens, building autonomous modulation of cell growth rate and culture composition in response to a quorum-sensing signal.
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Recent advances in producing food additive L‐malate: Chassis, substrate, pathway, fermentation regulation and application
Qiang Ding,Chao Ye +1 more
TL;DR: In this paper , the authors mainly focused on biotechnological approaches for enhancing Lmalate synthesis, encompassing the microbial chassis, substrate utilization, synthesis pathway, fermentation regulation, and industrial application.
Journal ArticleDOI
Microbial cell factories: a biotechnology journey across species.
TL;DR: In this paper, the authors present a collection of reviews of 18 microbial cell factories, which belong to one of these three groups: (i) microbes already used before modern biotechnology was introduced; (ii) the first generation of engineered microbes; and (iii) promising new host organisms.
References
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Lei S. Qi,Matthew H. Larson,Luke A. Gilbert,Jennifer A. Doudna,Jonathan S. Weissman,Adam P. Arkin,Adam P. Arkin,Wendell A. Lim +7 more
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What is flux balance analysis
TL;DR: This primer covers the theoretical basis of the approach, several practical examples and a software toolbox for performing the calculations.
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Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
TL;DR: This strategy uses the host’s highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis to achieve high-yield, high-specificity production of isobutanol from glucose.