Journal ArticleDOI
Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline.
Charles L. Armstrong,C. E. Green +1 more
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TLDR
Frequentencies of friable-callus initiation and somatic-embryoid formation increased linearly with addition to N6 medium, and L-Glutamine was not a satisfactory substitute for L-proline.Abstract:
Friable, embryogenic maize (Zea mays L.), inbred line A188, callus was established and maintained for more than one year without apparent loss of friability or embryogenic potential. Embryoid development was abundant in these cultures and plants were easily regenerated. Frequencies of friable-callus initiation and somatic-embryoid formation increased linearly with addition to N6 medium (C.C. Chu et al. 1975, Sci. Sin. [Peking] 18, 659–668) of up to 25 mM L-proline. Proline additions up to 9 mM to MS medium (inorganic elements of T. Murashige and F. Skoog 1962, Physiol. Plant. 15, 473–497, plus 0.5 mg 1-1 thiamine hydrochloride and 150 mg 1-1
L-asparagine monohydrate) did not stimulate embryoid formation. A major part of the difference between MS and N6 media could be attributed to their respective inorganic nitrogen components. L-Glutamine was not a satisfactory substitute for L-proline. Of 111 regenerated plants grown to maturity from three independent friable, embryogenic cell lines ranging in age from three to seven months, only four plants were abnormal based on morphology and pollen sterility. Seed was produced by 77% of the regenerated plants.read more
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Role of proline under changing environments: a review.
TL;DR: Review of the literature indicates that a stressful environment results in an overproduction of proline in plants which in turn imparts stress tolerance by maintaining cell turgor or osmotic balance; stabilizing membranes thereby preventing electrolyte leakage; and bringing concentrations of reactive oxygen species within normal ranges, thus preventing oxidative burst in plants.
Patent
Inbred corn plant 89AHD12 and seeds thereof
TL;DR: In this article, the authors provided an inbred corn plant designated 89AHD12, which was used to produce corn seeds and plants produced by crossing the inbred plant with another corn plant, such as another inbred, and to crosses with related species.
Patent
Inbred corn line LH283BtMON810
TL;DR: An inbred corn line, designated LH283BtMON810, is disclosed in this paper, which relates to the seeds of the inbred line and to the plants of the line.
Journal ArticleDOI
High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens
TL;DR: Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried “super-binary” vectors that carried stable integration, expression, and inheritance of the transgenes.
Patent
Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof
Thomas R. Adams,Sheryl A. Chambers,Richard J. Daines,William J. Gordon-Kamm,Albert P. Kausch,Peggy G. Lemaux,Catherine J. Mackey,Mary L. Mangano,James V. O'brien,Thomas B. Rice,T. Michael Spencer,William G. Start,Nancy Willetts +12 more
TL;DR: In this article, a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed, is described. But the system is not applicable to the field of agriculture.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Book
Principles and Procedures of Statistics: A Biometrical Approach
TL;DR: Observations probability sampling from a normal distribution comparisons involving two sample means principles of experimental design analysis of variance.
Journal ArticleDOI
Principles and Procedures of Statistics: A Biometrical Approach.
Journal ArticleDOI
Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources
Chih-Ching Chu,Ching-Chu Wang,Ching-San Sun,Chen Hsu,Kwang-Chu Yin,Chih--Yin Chu,Feng-Yun Bi +6 more
TL;DR: In this paper, it was shown that (NH4)2SO4 combined with KNO3 at low concentration is of advantage to the formation, growth and differentiation of pollen callus in rice.
Journal ArticleDOI
Plant Regeneration from Tissue Cultures of Maize1
C. E. Green,Ronald L. Phillips +1 more