Establishment of a Protein Reference Map for Soybean Root Hair Cells
Laurent Brechenmacher,Joohyun Lee,Sherri Weiss Sachdev,Zhao Song,Tran Hong Nha Nguyen,Trupti Joshi,Nathan Oehrle,Marc Libault,Brian P. Mooney,Dong Xu,Bret Cooper,Gary Stacey +11 more
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TLDR
Analysis of the proteome of isolated soybean root hair cells using two-dimensional polyacrylamide gel electrophoresis and shotgun proteomics provides useful insight into the metabolic activities of a single, differentiated plant cell type.Abstract:
Root hairs are single tubular cells formed from the differentiation of epidermal cells on roots. They are involved in water and nutrient uptake and represent the infection site on leguminous roots by rhizobia, soil bacteria that establish a nitrogen-fixing symbiosis. Root hairs develop by polar cell expansion or tip growth, a unique mode of plant growth shared only with pollen tubes. A more complete characterization of root hair cell biology will lead to a better understanding of tip growth, the rhizobial infection process, and also lead to improvements in plant water and nutrient uptake. We analyzed the proteome of isolated soybean (Glycine max) root hair cells using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and shotgun proteomics (1D-PAGE-liquid chromatography and multidimensional protein identification technology) approaches. Soybean was selected for this study due to its agronomic importance and its root size. The resulting soybean root hair proteome reference map identified 1,492 different proteins. 2D-PAGE followed by mass spectrometry identified 527 proteins from total cell contents. A complementary shotgun analysis identified 1,134 total proteins, including 443 proteins that were specific to the microsomal fraction. Only 169 proteins were identified by the 2D-PAGE and shotgun methods, which highlights the advantage of using both methods. The proteins identified are involved not only in basic cell metabolism but also in functions more specific to the single root hair cell, including water and nutrient uptake, vesicle trafficking, and hormone and secondary metabolism. The data presented provide useful insight into the metabolic activities of a single, differentiated plant cell type.read more
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The Root Hair “Infectome” of Medicago truncatula Uncovers Changes in Cell Cycle Genes and Reveals a Requirement for Auxin Signaling in Rhizobial Infection
Andrew Breakspear,Cheng-Wu Liu,Sonali Roy,Nicola Stacey,Christian Rogers,Martin Trick,Giulia Morieri,Kirankumar S. Mysore,Jiangqi Wen,Giles E. D. Oldroyd,J. Allan Downie,Jeremy D. Murray +11 more
TL;DR: It is concluded that the onset of infection is associated with reactivation of the cell cycle as well as increased expression of genes required for hormone and flavonoid biosynthesis and that the regulation of auxin signaling is necessary for initiation of rhizobial infection threads.
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Integrating omic approaches for abiotic stress tolerance in soybean.
Rupesh Deshmukh,Humira Sonah,Gunvant Patil,Wei Chen,Silvas J. Prince,Raymond N. Mutava,Tri D. Vuong,Babu Valliyodan,Henry T. Nguyen +8 more
TL;DR: This review has described advances in omic tools in the view of conventional and modern approaches being used to dissect abiotic stress tolerance in soybean and addressed the significance of phenomics in the integrated approaches and recognized high-throughput multi-dimensional phenotyping as a major limiting factor for the improvement of abiotic Stress tolerance in Soybean.
Journal ArticleDOI
Soybean Metabolites Regulated in Root Hairs in Response to the Symbiotic Bacterium Bradyrhizobium japonicum
Laurent Brechenmacher,Zhentian Lei,Marc Libault,Seth D. Findley,Masayuki Sugawara,Michael J. Sadowsky,Lloyd W. Sumner,Gary Stacey +7 more
TL;DR: In this article, a metabolomic study was performed to identify small molecules produced in roots and root hairs during the rhizobial infection process, and trehalose was among the most strongly induced metabolites produced following inoculation.
Journal ArticleDOI
Root hair systems biology
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Journal ArticleDOI
Studies of a Biochemical Factory: Tomato Trichome Deep Expressed Sequence Tag Sequencing and Proteomics
Anthony L. Schilmiller,Dennis P. Miner,Matthew D. Larson,Eric T. McDowell,David R. Gang,Curtis G. Wilkerson +5 more
TL;DR: The utility of combining high-throughput cDNA sequencing with proteomics experiments in a target tissue and the discovery and characterization of a sesquiterpene synthase that produces β-caryophyllene and α-humulene from E,E-farnesyl diphosphate in trichomes of leaf but not of stem is demonstrated.
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