Journal ArticleDOI
Establishment of AFLP technique and assessment of primer combinations for mei flower
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TLDR
AFLP is an effective, inexpensive, and timesaving technique for the genetic differentiation of the Mei cultivars, as evidenced in this study.Abstract:
Mei flower is one of the most famous ornamental flowers in eastern Asia for its blossoming in early spring. Amplified fragment length polymorphism (AFLP) is one of the most frequently used techniques for analysis of genetic variation and is used herein for the first time inPrunus mume. This research provides a detailed and modified AFLP protocol for Mei genomic DNA digested withEcoRI/PstI restriction endonuclease combinations. The 10 best primer pairs of high polymorphism were screened from 256 primer combinations that could reliably and repetitively distinguish 14 Mei samples and would be suitable for genetic analysis of more cultivars. Ten primer pairs produced up to a total of 524 AFLP bands and up to 233 polymorphic bands. The ratio of polymorphic bands scoped from 35.71% to 59.67%, and the average ratio was 44.46% in the 10 primers. AFLP is an effective, inexpensive, and timesaving technique for the genetic differentiation of the Mei cultivars, as evidenced in this study.read more
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Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning
TL;DR: A simple and effective fusion primer and nested integrated PCR method (FPNI-PCR) for the identification and cloning of unknown genomic regions flanked known sequences and verified that this novel strategy is an effective, low cost and simple procedure.
Journal ArticleDOI
The genetic architecture of floral traits in the woody plant Prunus mume
Qixiang Zhang,He Zhang,Lidan Sun,Guangyi Fan,Meixia Ye,Libo Jiang,Xin Liu,Kaifeng Ma,Chengcheng Shi,Fei Bao,Rui Guan,Han Yu,Yuanyuan Fu,Huitang Pan,Zhaozhe Chen,Liangwei Li,Jia Wang,Meiqi Lv,Tangchun Zheng,Cunquan Yuan,Yuzhen Zhou,Simon Ming-Yuen Lee,Xiaolan Yan,Xun Xu,Rongling Wu,Wenbin Chen,Tangren Cheng +26 more
TL;DR: This study samples wild and domesticated mei to reveal considerable admixture and introgression from other Prunus species and identifies loci associated with floral traits, and identifies significant quantitative traits locus (QTLs) and genomic regions where several genes are positively associated with petal color, stigma color, calyx color, and bud color.
Journal ArticleDOI
Embryo rescue and plant regeneration following interspecific crosses in the genus Hylocereus (Cactaceae)
Aroldo Cisneros,Noemi Tel-Zur +1 more
TL;DR: The three-step embryo rescue procedure developed here provides the means for producing plants from very-early embryo stage, thus expanding the prospects for vine-cactus breeding programs.
Journal ArticleDOI
Shortening tobacco life cycle accelerates functional gene identification in genomic research.
TL;DR: Comparative analysis among wild type, short life cycle tobacco and Arabidopsis transformation system verified that it is optional to accelerate functional gene studies by shortening host plant material life cycle, at least in these shortlife cycle tobacco lines.
Journal Article
Micropropagation of six prunus mume cultivars through axillary shoot proliferation, and issr analysis of cloned plants
TL;DR: In vitro culture, a set of techniques that offers a tool for mass multiplication, germplasm conservation and transformation research, has been widely used in Prunus species, but very few reports have described micropropagation of P. mume through in vitro culture.
References
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Journal ArticleDOI
AFLP: a new technique for DNA fingerprinting.
Pieter Vos,René Cornelis Josephus Hogers,Marjo Bleeker,Martin Reijans,Theo van de Lee,Miranda Hornes,Adrie Friters,Jerina Pot,Johan Paleman,Martin Kuiper,Marc Zabeau +10 more
TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
Journal ArticleDOI
A plant DNA minipreparation: Version II
TL;DR: The topic of this report is rap,d m,croscale methods for,solat,on of plant D N A without tile use of ul tracentr ,fugatlon wEth CsCI, which is of moderately high molecular weight and serves as a satisfactory substrate for most restrlctum cndonucleases and is statable for genom,c blot analys,s.
Journal ArticleDOI
Nuclear DNA Amounts in Angiosperms
TL;DR: This paper lists absolute nuclear DNA amounts for 753 angiosperm species, and the dats were assembled primarily for reference purposes, and so the species are listed in alphabetical order, as this was felt to be more helpful to cyto- and biochemists whom, it is anticipated, will be among its major users.
Journal ArticleDOI
Nuclear DNA Amounts in Angiosperms
TL;DR: This paper contains a supplementary list of absolute DNA values, including estimates for 240 angiosperm species not listed by Bennett & Smith in 1976, as well as additional estimates for 41 species already listed by them.
Journal ArticleDOI
Comparative mapping and marker-assisted selection in Rosaceae fruit crops
Elisabeth Dirlewanger,Enrique Graziano,Tarek Joobeur,Francesc Garriga-Calderé,Patrick Cosson,Werner Howad,Pere Arús +6 more
TL;DR: By using the data from different linkage maps anchored with the reference Prunus map, it has been possible to establish, in a general map, the position of 28 major genes affecting agronomic characters found in different species.
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