Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources
Chih-Ching Chu,Ching-Chu Wang,Ching-San Sun,Chen Hsu,Kwang-Chu Yin,Chih--Yin Chu,Feng-Yun Bi +6 more
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TLDR
In this paper, it was shown that (NH4)2SO4 combined with KNO3 at low concentration is of advantage to the formation, growth and differentiation of pollen callus in rice.Abstract:
The experiments have revealed that (NH4)2SO4 combined with KNO3 at low concentration is of advantage to the formation, growth and differentiation of pollen callus in rice, whereas the high concentration of (NH4)2SO4, whether used separately or in combination with KNO3, obviously inhibits the pollen callus formation. The optimum NH4+ concen- tration is about 7.0 mM (equal to 3.5 mM (NH4)2SO4). A basic medium containing 3.5 mM (NH4)2SO4 and 28 mM KNO3 as nitrogen sources has been established. On such medium the frequency of the pollen callus formation is higher than that on Millers me- dium, and the differentiation of shoot from pollen callus is satisfactory.read more
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Journal ArticleDOI
Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
W. J. Gordon-Kamm,T. M. Spencer,Mary L. Mangano,Thomas R. Adams,Richard J. Daines,William G. Start,James V. O'brien,Sheryl A. Chambers,W. R. Adams,Nancy Willetts,Thomas B. Rice,Catherine J. Mackey,R. W. Krueger,Albert P. Kausch,Peggy G. Lemaux +14 more
TL;DR: A reproducible system for the generation of fertile, transgenic maize plants has been developed and activity of the enzyme phosphinothricin acetyltransferase (PAT) encoded by bar were confirmed in all bialaphos-resistant callus lines.
Journal ArticleDOI
Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline.
Charles L. Armstrong,C. E. Green +1 more
TL;DR: Frequentencies of friable-callus initiation and somatic-embryoid formation increased linearly with addition to N6 medium, and L-Glutamine was not a satisfactory substitute for L-proline.
Journal ArticleDOI
The production of callus capable of plant regeneration from immature embryos of numerous Zea mays genotypes.
TL;DR: Tissue-culture techniques are now available to obtain callus cultures capable of plant regeneration from immature embryos of most maize genotypes, and plant regeneration was noted in many commercially important inbreds.
Journal ArticleDOI
Iron fortification of rice seed by the soybean ferritin gene.
TL;DR: The rice seed-storage protein glutelin promoter, GluB-1, was used to drive expression of the soybean gene specifically in developing, self-pollinated seeds (T1 seeds) of transgenic plants, as confirmed by reverse transcription PCR analysis.
Patent
Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof
Thomas R. Adams,Sheryl A. Chambers,Richard J. Daines,William J. Gordon-Kamm,Albert P. Kausch,Peggy G. Lemaux,Catherine J. Mackey,Mary L. Mangano,James V. O'brien,Thomas B. Rice,T. Michael Spencer,William G. Start,Nancy Willetts +12 more
TL;DR: In this article, a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed, is described. But the system is not applicable to the field of agriculture.
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