Estimation of conjunctival swab and nasopharyngeal swab specimens for viral nucleic acid detection in Coronavirus disease 2019 patients to compare the viral load
Jaya Kaushik,Vikas Marwah,Ankita Singh,Y V K Chaitanya,Rajeev Mohan Gupta,Poninder Kumar +5 more
- Vol. 4, pp 2
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TLDR
The results of the study revealed that SARS-CoV-2 can also be detected in conjunctival swabs of confirmed cases of COVID-19 patients, and in comparison to nasopharyngeal and throat swabs the rate of detection is relatively less, still diligent care and precautions should be practiced during the ophthalmic evaluation of COIDs patients.Abstract:
Objectives: The purpose of the study was to detect the presence of viral ribonucleic acid of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in conjunctival swab along with nasopharyngeal swab specimens of Coronavirus disease 2019 (COVID-19) patients. Material and Methods: Thirty COVID-19 patients with at least one sample positive for real-time reverse transcription-polymerase chain reaction for SARS-CoV-2 in nasopharyngeal swab with the presence or absence of ocular manifestations were included in the study. The conjunctival swab along with nasopharyngeal swab of each patient was collected and sent to microbiology lab for evaluation and analysis of viral nucleic acid to assess the viral load. Results: Out of 30 patients, 21 patients (70%) were males and the remaining nine patients (30%) were females. Mean age of the patients in the study was 44.80 ± 15.37 years. One patient had conjunctivitis as ocular manifestation. Two (6.7%) out of 30 patients were positive for RT-PCR SARS-CoV-2 in the conjunctival swab. There was no statistical correlation between nasopharyngeal swab and conjunctival swab positivity using Pearson’s correlation coefficient (r) = 0.010; P = 0.995 (>0.05). Conclusion: The results of the study revealed that SARS-CoV-2 can also be detected in conjunctival swabs of confirmed cases of COVID-19 patients. Although, in comparison to nasopharyngeal and throat swabs the rate of detection of SARS-CoV-2 in conjunctival swabs is relatively less, still diligent care and precautions should be practiced during the ophthalmic evaluation of COVID-19 patients.read more
References
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Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
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Defining the Epidemiology of Covid-19 - Studies Needed.
TL;DR: Defining the Epidemiology of Covid-19 Experience with MERS, pandemic influenza, and other outbreaks has shown that as an epidemic evolves, there is an urgent need to expand public health activities in response to this epidemic.
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Evaluation of coronavirus in tears and conjunctival secretions of patients with SARS-CoV-2 infection.
TL;DR: Wang et al. as mentioned in this paper assessed the presence of novel coronavirus in tears and conjunctival secretions of SARS-CoV-2-infected patients, and they concluded that SARS may be detected in conjunctivitis.
Journal Article
Evaluation of coronavirus in tears and conjunctival secretions of patients with SARS-CoV-2 infection
TL;DR: This study aimed to assess the presence of novel coronavirus in tears and conjunctival secretions of SARS–CoV‐2‐infected patients.
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SARS-CoV-2 invades host cells via a novel route: CD147-spike protein
Ke Wang,Wei Chen,Yu-Sen Zhou,Jian-Qi Lian,Zheng Zhang,Peng Du,Li Gong,Yang Zhang,Hong-Yong Cui,Jie-Jie Geng,Bin Wang,Xiu-Xuan Sun,Chun-Fu Wang,Xu Yang,Peng Lin,Yong-Qiang Deng,Ding Wei,Xiang-Min Yang,Yu-Meng Zhu,Kui Zhang,Zhaohui Zheng,Jinlin Miao,Ting Guo,Ying Shi,Jun Zhang,Ling Fu,Qing-Yi Wang,Huijie Bian,Ping Zhu,Zhi-Nan Chen +29 more
TL;DR: The discovery of the new route CD147-SP for SARS-CoV-2 invading host cells provides a critical target for development of specific antiviral drugs.
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