Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
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TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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Insights into removal mechanisms of bisphenol A and its analogues in municipal wastewater treatment plants
TL;DR: The theoretically predicted removal of BPA in municipal WWTP showed that it is readily biodegradable, which deviate from its on-site investigations, which showed that sludge adsorption played an important role on BPA removal.
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Expression profiling of estrogen-responsive genes in breast cancer cells treated with alkylphenols, chlorinated phenols, parabens, or bis- and benzoylphenols for evaluation of estrogenic activity
TL;DR: The results indicate that the variations in chemicals and their biological effects can be monitored by the appropriate grouping of estrogen-responsive genes, and that the genes related to transcription showed the highest degree of variation for the chemicals with relatively high levels of estrogenic activity.
Journal ArticleDOI
Evaluation of Estrogenic Activities of Aquatic Herbicides and Surfactants Using an Rainbow Trout Vitellogenin Assay
Lingtian Xie,Kelly Thrippleton,Mary Ann Irwin,G. Siemering,Abdou Mekebri,D. Crane,Kevin Berry,Daniel Schlenk +7 more
TL;DR: These results demonstrated the binary mixture of alkylphenol ethoxylate-containing surfactants with two aquatic pesticides possessed greater than additive estrogenic responses in fish under laboratory conditions and in a field setting.
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Detection of Thyroid System–Disrupting Chemicals Using in Vitro and in Vivo Screening Assays in Xenopus laevis
TL;DR: A thyroid hormone (TH) inducible primary screening assay for the identification and assessment of man-made chemicals that interfere with the TH-signalling pathway within target cells and results indicate that the luciferase assay was a rapid method with a small intra-assay variation for the primary screening of thyroid system-disruption chemicals.
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.