Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
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TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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Toxicological profile for DDT, DDE, and DDD
Obaid Faroon,M. Olivia Harris +1 more
TL;DR: Each toxicological profile succinctly characterizes the toxicologic and adverse health effects information for the hazardous substance described therein and begins with a public health statement that describes, in nontechnical language, a substance's reevant toxicological properties.
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Organotin Compounds Promote Adipocyte Differentiation as Agonists of the Peroxisome Proliferator-Activated Receptor γ/Retinoid X Receptor Pathway
TL;DR: It is found that triphenyltin and tributyltin were activators of peroxisome proliferator-activated receptor (PPAR) γ and retinoid X receptor and organotin compounds stimulated differentiation of 3T3-L1 cells as well as expression of adipocyte marker genes.
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ER-dependent estrogenic activity of parabens assessed by proliferation of human breast cancer MCF-7 cells and expression of ERα and PR
TL;DR: Parabens have ER-dependent estrogenic activities, and their effects on the intracellular signaling pathway might be different from that of 17beta-estradiol.
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Spoilt for choice: A critical review on the chemical and biological assessment of current wastewater treatment technologies.
TL;DR: This review summarizes the current approaches used to assess treated wastewater quality from the chemical and ecotoxicological perspective and critically discusses the benefits and limitations of the different methodologies reviewed.
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Parabens as urinary biomarkers of exposure in humans.
TL;DR: The results, demonstrating the presence of urinary conjugates of paraben in humans, suggest that such conjugated parabens could be used as exposure biomarkers for human exposure to these compounds.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.