Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
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TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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Estrogenic activity in sediments contaminated by nonylphenol in Tokyo Bay (Japan) evaluated by vitellogenin induction in male mummichogs (Fundulus heteroclitus).
TL;DR: The induction of Vtg was observed; this estrogenic potency could be attributed to the NP content in this fraction, the first report to suggest that the high NP concentration in the sediments from Tokyo Bay has the potential to induce Vtg in wild fish.
Journal ArticleDOI
Pharmacokinetics and metabolism of benzophenone 2 in the rat
TL;DR: The amount of unconjugated BP2 was sufficient to induce a dose dependent estrogenic effect in the uterotrophic assay, and it is suggested that this biotransformation occurs via a first-passEffect in the gut wall or the liver.
Journal ArticleDOI
Subacute oral toxicity study of di(2-ethylhexyl)adipate based on the draft protocol for the “Enhanced OECD Test Guideline no. 407”
Katsumi Miyata,Keiji Shiraishi,Satsuki Houshuyama,Nobuya Imatanaka,Takaaki Umano,Yasushi Minobe,Kanji Yamasaki +6 more
TL;DR: A 28-day repeated-dose toxicity study of di(2-ethylhexyl)adipate based on the draft protocol of the “Enhanced OECD Test Guideline 407” to investigate whether it has endocrine-mediated properties according to this assay found disturbance of the estrous cycle and increased ovarian follicle atresia in the 1,000 mg/kg group.
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Induction of Plasma Vitellogenin Synthesis by the Commercial Fish Diets in Male Goldfish (Carassius auratus) and Dietary Phytoestrogens
Hiroshi Ishibashi,Makito Kobayashi,Tomohiko Koshiishi,Toshihisa Moriwaki,Katsuyasu Tachibana,Mutsuyosi Tsuchimoto,Kiyoshi Soyano,Taisen Iguchi,Chisato Mori,Koji Arizono +9 more
TL;DR: The formulated diet developed in the present study contain less phytoestrogens than the commercial fish diets and has low estrogenic activity, and it is suggested that VTG production using male goldfish in combination with the low estrogen fish diet is a good in vivo system for evaluation of estrogenic effects of EDCs.
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The effect of bisphenol A and chlorinated derivatives of bisphenol A on the level of serum vitellogenin in Japanese medaka (Oryzias latipes).
Akihisa Tabata,N. Watanabe,I. Yamamoto,Yuta Ohnishi,M. Itoh,Tasuku Kamei,Yasumoto Magara,Y. Terao +7 more
TL;DR: The decrease in BPA and its chlorinated derivatives paralleled the decrease in estrogenic potency evaluated by the induction of vitellogenin (VTG) in the serum of mature male Japanese medaka.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.