Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay
Tsutomu Nishihara,Jun-ichi Nishikawa,Tomohiko Kanayama,Fumi Dakeyama,Koichi Saito,Masayoshi Imagawa,Satoshi Takatori,Yoko Kitagawa,Shinjiro Hori,Hideo Utsumi +9 more
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TLDR
A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.Abstract:
One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.read more
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New approach methods for testing chemicals for endocrine disruption potential
Richard S. Judson,Katie Paul Friedman,Keith A. Houck,Kamel Mansouri,Patience Browne,Nicole Kleinstreuer +5 more
TL;DR: This review of efforts at the US EPA and the National Toxicology Program to develop assays and models to predict estrogen, androgen and steroidogenesis activity shows these approaches have been shown to be robust enough to move from research to application in regulatory risk assessment.
Journal ArticleDOI
A two-generation reproductive toxicity study of benzophenone in rats
TL;DR: The reproductive toxicity of benzophenone (BZP) was evaluated in a two generation test in which male and female Sprague-Dawley rats, parental (F0) and first generation (F1), were exposed to BZP by feeding diet containing BzP at concentrations of 0, 100, 450 or 2000 ppm.
Journal ArticleDOI
Determination of estrogenic activity in landfill leachate by simplified yeast two-hybrid assay.
TL;DR: A simplified yeast two-hybrid assay of estrogenic activity was used to test various chemicals suspected of estrogenicity and detected in leachate from a municipal waste-dumping site, but not in that from a dredged soil-dumped site, which did however cause inhibition of cell growth.
Journal ArticleDOI
A polycyclic aromatic hydrocarbon-enriched environmental chemical mixture enhances AhR, antiapoptotic signaling and a proliferative phenotype in breast cancer cells.
Larisa M. Gearhart-Serna,Larisa M. Gearhart-Serna,John B Davis,Mohit Kumar Jolly,Nishad Jayasundara,Nishad Jayasundara,Scott J Sauer,Richard T. Di Giulio,Gayathri R. Devi,Gayathri R. Devi +9 more
TL;DR: The underlying mechanisms of EDC mixtures in breast cancer progression and survival are revealed, with integrated cell signaling, computational and phenotype analysis revealing the underlying mechanisms.
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Antiestrogenic effect of paradichlorobenzene in immature mice and rats
TL;DR: The results suggest that PDCB is a weak antiestrogenic/antiuterotrophic compound possibly due to ER modulation through arylhydrocarbon receptor.
References
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Book
Our Stolen Future
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.