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Journal Article•DOI•

Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay

Tsutomu Nishihara1, Jun-ichi Nishikawa, Tomohiko Kanayama1, Fumi Dakeyama1, Koichi Saito2, Masayoshi Imagawa, Satoshi Takatori, Yoko Kitagawa, Shinjiro Hori, Hideo Utsumi3 •
Osaka University1, Nagoya City University2, Kyushu University3
01 Aug 2000-Journal of Health Science (The Pharmaceutical Society of Japan)-Vol. 46, Iss: 4, pp 282-298
TL;DR: A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.
Abstract: One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.

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Citations
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Journal Article•DOI•
Fate of estrogenic hormones in wastewater and sludge treatment: A review of properties and analytical detection techniques in sludge matrix.

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Hanna Hamid1, Cigdem Eskicioglu1•
University of British Columbia1
15 Nov 2012-Water Research
TL;DR: Although, aerobic digestion showed better attenuation of estrogenic compounds, anaerobic digestion increased the overall estrogenicity of biosolids, and this paper highlights the challenges involved in analytical determination of these compounds in sewage sludge matrix.

231 citations

Cites background from "Estrogenic Activities of 517 Chemic..."

  • ...In contrast, thermophilic and mesophilic anaerobic digestion resulted a net increase in estrogenicity as measured by YES bioassay and the contribution of estrogenic hormones to total estrogenicity more than doubled after anaerobic digestion....

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  • ...AMO, Ammonium mono-oxygenase; AOB, Ammonia oxidizing bacteria; APCI, Atmospheric ed solvent extraction; BOD, Biochemical oxygen demand; C, Total compound concentration; ion in water; CAS, Chemical abstract services; CEE, Conjugated Equine Estrogens; DTE, iol; E3, Estriol; EE2, 17a-ethinylestradiol; EDC, Endocrine disrupting compound; EDSP, A, Endocrine Disruptors Testing and Assessment; Eq, Equilin; Eqn, Equilenin; ER, Estrogen s chromatography; GLU, Glucuronide; GPC, Gel permeation chromatography; H, Henry’s law h, Hormone replacement therapy; k, Reaction rate constant; Kd, Distribution coefficient; KF, fficient normalized to organic matter; KOC, Distribution coefficient normalized to organic n coefficient; LC, Liquid chromatography; LDTD, Laser diode thermal desorption; MAE, ectrometry; MSTFA, N-(trimethylsilyl) trifluoroacetamide; NAS, Nitrifying activated sludge; ganization for Economic Cooperation and Development; pKa, Acid dissociation constant; nsport Evaluation; PPCP, Pharmaceutical and personal care product; S, Soluble compound PEED, Strategic Programs on Endocrine Disruptors; SRT, Solid retention time; SS, Suspended lsilylimidazole; TSS, Total suspended solids concentration; USEPA, United States Environspended solids concentration; WWTP, Wastewater treatment plant; YES, Yeast estrogen 4; fax: þ1 250 8079850....

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  • ...Their findings suggested aerobic digestion removed most of the natural and synthetic estrogenic compounds successfully and corresponded to 18% reduction of estrogenicity observed in the YES bioassay....

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  • ...YES utilizes a human estrogen receptor recombinant engineered with a beta-galactosidase reporter (Routledge and Sumpter, 1996)....

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  • ...b Estrogenic potency determined by yeast estrogen screen (YES) (Nishihara et al., 2000)....

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Journal Article•DOI•
Environmental oestrogens, cosmetics and breast cancer.

[...]

Philippa D. Darbre1•
University of Reading1
01 Mar 2006-Best Practice & Research Clinical Endocrinology & Metabolism
TL;DR: Evidence in support of a functional role for the combined interactions of cosmetic chemicals with environmental oestrogen, pharmacological oestrogens, phyto-oestrogens and physiological oestosterone in the rising incidence of breast cancer is considered.

227 citations

Journal Article•DOI•
Effects of butyl paraben on the male reproductive system in mice.

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Shinshi Oishi
13 Jun 2002-Archives of Toxicology
TL;DR: It is shown that butyl paraben had an adverse effect on the male mouse reproductive system and that it damaged the late steps of spermatogenesis in the testis and the serum testosterone concentration decreased in a dose-dependent fashion.
Abstract: Parabens are alkyl ester compounds of p-hydroxybenzoic acid widely used as preservatives in foodstuffs, cosmetics, toiletries and pharmaceuticals. These compounds are known to exert a weak estrogenic activity in estrogen receptor assays in vitro and in uterotrophic assays in vivo. In this paper, we have shown that butyl paraben had an adverse effect on the male mouse reproductive system and that it damaged the late steps of spermatogenesis in the testis. Butyl paraben was administered to 4-week-old Crj:CD-1 mice assigned to groups of eight animals, at doses of 0.01%, 0.10%, and 1.00% in the diet for 10 weeks. The average butyl paraben intake from the calculated food consumption was 14.4+/-3.60, 146+/-35.9, and 1504+/-357 mg/kg per day for the 0.01%, 0.10%, and 1.00% dietary butyl paraben groups, respectively. There were no treatment-related effects of butyl paraben on the liver, ventral prostates, seminal vesicles, and preputial glands (both in terms of absolute weight and relative to body weight) in any of the study groups. Both the absolute and relative weights of the epididymides were significantly higher in 1.00% group when compared with controls. A dose-dependent decrease of both round and elongated spermatid counts in stages VII-VIII seminiferous tubules was observed, and the elongated spermatid counts were significantly lower in all of the treated groups. The numbers of spermatogonia and spermatocytes did not differ from control values. The serum testosterone concentration decreased in a dose-dependent fashion and was significant at 1.00%. These data demonstrated that butyl paraben can exert an adverse effect on the male reproductive system at doses that are well below those of the accepted daily intake (ADI) in Japan.

224 citations

Journal Article•DOI•
Estrogenic activity of 37 components of commercial sunscreen lotions evaluated by in vitro assays.

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Kaori Morohoshi1, Hiroshi Yamamoto2, Ryo Kamata1, Fujio Shiraishi1, Tomoko Koda1, Masatoshi Morita1 •
National Institute for Environmental Studies1, University of Tokushima2
01 Jun 2005-Toxicology in Vitro
TL;DR: In vivo testing of the compounds reported here to have estrogen agonistic and antagonistic activities is required to confirm their effects at an organismal level.

193 citations

Journal Article•DOI•
Urinary excretion rates of natural estrogens and androgens from humans, and their occurrence and fate in the environment: a review.

[...]

Zehua Liu1, Yoshinori Kanjo1, Satoshi Mizutani1•
Osaka City University1
01 Sep 2009-Science of The Total Environment
TL;DR: Five out of 12 natural androgens, T, dihydrotestosterone (DHT), androsterone (AD), 5beta-androstanediol (beta-ADL), and androstenediol (ANL) were evaluated as the priority natural androgenes, which may exist in wastewater with high androgenic activities.

190 citations

References
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Book•
Our Stolen Future

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Theo Colborn, Dianne Dumanoski, John Peterson Myers
01 Jan 1996
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.
Abstract: For years, scientists have noticed disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure. Humans are not immune either, with sperm counts dropping by as much as 50% in recent decades and with women seeing a rise in hormone-related cancers, endometriosis and other disorders. This book traces the cause of these aberrations and diseases to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system. The conclusions are as obvious as they are inescapable - unless we make vital changes in the way we manufacture and employ the artefacts of our "good life", there will be no life at all.

917 citations

Related Papers (5)
The Estrogen Receptor Relative Binding Affinities of 188 Natural and Xenochemicals: Structural Diversity of Ligands

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01 Mar 2000-Toxicological Sciences
R. Blair, Hong Fang, William S. Branham, Bruce S. Hass, Stacey L. Dial, Carrie L. Moland, Weida Tong, Leming Shi, Roger Perkins, Daniel M. Sheehan 
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01 Oct 1995-Environmental Health Perspectives
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01 Mar 1996-Environmental Toxicology and Chemistry
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Developmental effects of endocrine-disrupting chemicals in wildlife and humans.

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01 Oct 1993-Environmental Health Perspectives
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A variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic.

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