scispace - formally typeset
Search or ask a question
Journal Article•DOI•

Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay

Tsutomu Nishihara1, Jun-ichi Nishikawa, Tomohiko Kanayama1, Fumi Dakeyama1, Koichi Saito2, Masayoshi Imagawa, Satoshi Takatori, Yoko Kitagawa, Shinjiro Hori, Hideo Utsumi3 •
Osaka University1, Nagoya City University2, Kyushu University3
01 Aug 2000-Journal of Health Science (The Pharmaceutical Society of Japan)-Vol. 46, Iss: 4, pp 282-298
TL;DR: A simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators to test the estrogenic activity of chemicals.
Abstract: One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.

Content maybe subject to copyright    Report

Citations
More filters
Other•DOI•
Occurrence of endocrine active compounds and biological responses in the Mississippi River; study design and data, June through August 2006

[...]

Kathy E. Lee, Christine S. Yaeger, Nathan D. Jahns, Heiko L. Schoenfuss
01 Dec 2008

8 citations

Dissertation•
Oxydation chimique in situ de la zone non saturée de sols contaminés par du goudron de houille : du laboratoire au terrain

[...]

Bérénice Ranc
23 Jun 2017
TL;DR: In this paper, the authors investigated the effect of the use of thermique de loxydant on the efficiency of the traitement of a friche in the goudron de houille.
Abstract: Il existe en France des centaines de friches industrielles polluees par du goudron de houille, un melange recalcitrant de composes tels que les hydrocarbures aromatiques polycycliques. Lorsque la zone non saturee des sols est fortement contaminee, elle est usuellement excavee et remblayee. Cette these porte sur un traitement alternatif permettant une valorisation potentielle des sols sur site : l’oxydation chimique in situ, qui a deja montre des resultats encourageants au laboratoire mais n’a que tres peu ete testee en grandeurs reelles. Les recherches ont donc ete menees autour de trois echelles – bibliographie, laboratoire et pilote – afin de determiner s’il existait un traitement oxydant repondant a des criteres techniques, economiques et environnementaux acceptables pour etre appliquee a l’echelle de la friche. La phase laboratoire a montre que l’ajout d’un soutien thermique augmentait significativement l’efficacite du traitement, i) par augmentation de la disponibilite de la pollution par prechauffage de la terre dans le cas du permanganate, ou ii) par activation thermique de l’oxydant dans le cas du persulfate. A l’echelle du pilote, une mise en contact homogene entre l’oxydant et la pollution n’a ete possible que par noyage partiel de la terre avec les solutions oxydantes concentrees. L’activation du persulfate s’est revelee delicate a mettre en œuvre, le chauffage de solutions concentrees ayant mene a une decomposition parasite de l’oxydant. Au contraire, l’utilisation de solutions concentrees de permanganate a conduit a une degradation des polluants encore plus elevee qu’au laboratoire grâce a la forte exothermicite de la reaction

8 citations

Journal Article•DOI•
Determination of Alkylphenols in Water by Dispersive Liquid–Liquid Microextraction Based on Solid Formation without a Disperser

[...]

Yu-Hsiang Sung, Chih-Hao Liu1, Mei-I Leong, Shang-Da Huang1•
National Tsing Hua University1
23 Jun 2014-Analytical Letters
TL;DR: Dispersive liquid-liquid microextraction based on solid formation without a disperser combined with high-performance liquid chromatography has been developed for the determination of 4-tert-butylphenol, 4-n-nonylphenol and 4-TERT-octylphenols.
Abstract: Dispersive liquid–liquid microextraction based on solid formation without a disperser combined with high-performance liquid chromatography has been developed for the determination of 4-tert-butylphenol, 4-n-nonylphenol, and 4-tert-octylphenol. This method is rapid, easy, and uses only 10 µL of a low toxicity organic solvent (1-hexadecanethiol) for the extraction solvent and no disperser solvent. The extraction time and centrifugation time require less than 10 min. The linear range was 1–500 ng mL−1 for 4-tert-butylphenol, 2–1000 ng mL−1 for 4-tert-octylphenol, and 5–500 ng mL−1 for 4-n-nonylphenol with r2 ≥ 0.9986. The detection limits were between 0.2 and 1.5 ng mL−1. The recoveries of lake and river water samples were in the range of 79% to 108%, and the relative standard deviations were 5% to 10%.

8 citations

Cites background from "Estrogenic Activities of 517 Chemic..."

  • ...1997), and in yeast two-hybrid assays (Nishihara et al. 2000)....

    [...]

  • ...Para-substituted alkylphenols have been shown to be estrogenic in fish and fish cells (Jobling and Sumpter 1993), rodents and human MCF-7 breast cancer cells (White et al. 1994; Nagel et al. 1997), and in yeast two-hybrid assays (Nishihara et al. 2000)....

    [...]

Journal Article•DOI•
In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox

[...]

Marie-Léonie Bohlen1, Hyun Pyo Jeon1, Young Jun Kim1, Baeckkyoung Sung1•
Kigali Institute of Science and Technology1
28 Aug 2019-Journal of Visualized Experiments
TL;DR: This study presents an in silico method to generate prediction data on the effects of representative EDs in aquatic vertebrates, particularly fish species using the automated workflow of the QSAR Toolbox software developed by the Organization for Economic Co-operation and Development.
Abstract: Computational analyses of toxicological processes enables high-throughput screening of chemical substances and prediction of their endpoints in biological systems. In particular, quantitative structure-activity relationship (QSAR) models have been increasingly applied to assess the environmental effects of a plethora of toxic materials. In recent years, some more highlighted types of toxicants are endocrine disruptors (EDs, which are chemicals that can interfere with any hormone-related metabolism). Because EDs may significantly affect animal development and reproduction, rapidly predicting the adverse effects of EDs using in silico techniques is required. This study presents an in silico method to generate prediction data on the effects of representative EDs in aquatic vertebrates, particularly fish species. The protocol describes an example utilizing the automated workflow of the QSAR Toolbox software developed by the Organization for Economic Co-operation and Development (OECD) to enable acute ecotoxicity predictions of EDs. As a result, the following are determined: (1) calculation of the numerical correlations between the concentration for 50% of lethality (LC50) and octanol-water partition coefficient (Kow), (2) output performances in which the LC50 values determined in experiments are compared to those generated by computations, and (3) the dependence of estrogen receptor binding affinity on the relationship between Kow and LC50.

8 citations

Journal Article•DOI•
RIFM fragrance ingredient safety assessment, Benzyl alcohol, CAS Registry Number 100-51-6.

[...]

A.M. Api1, D. Belsito2, S.P. Bhatia1, Magnus Bruze3, Peter Calow4, Maria Lúcia Zaidan Dagli5, W. Dekant6, Allison D. Fryer7, L Kromidas1, S. La Cava1, J F Lalko1, A. Lapczynski1, D. C. Liebler8, Valerie T. Politano1, G. Ritacco1, D. Salvito1, Terry W Schultz9, Jie Shen1, I. G. Sipes10, B Wall1, D K Wilcox1 •
Research Institute for Fragrance Materials1, Columbia University Medical Center2, Malmö University3, University of Nebraska–Lincoln4, University of São Paulo5, University of Würzburg6, Oregon Health & Science University7, Vanderbilt University8, University of Tennessee9, University of Arizona10
01 Oct 2015-Food and Chemical Toxicology
TL;DR: RIFM fragrance ingredient safety assessment, Benzyl alcohol, CAS Registry Number 100-51-6 A.M.W. schultz, J.F. Shen, I.G.Sipes, B.K. Wall, D.D. Liebler, and V.T. Politano.

8 citations

Cites background from "Estrogenic Activities of 517 Chemic..."

  • ...…1966; Schafer and Bowles, 1985; Taylor et al., 1964; Hoshino, 1940; Schweinsberg et al., 1986; MacEwen, 1986; Abramovici and Rachmuth-Roizman, 1983; Nishihara et al., 2000; SCCNFP, 2002; WHO, 2000; Kreis et al., 1971; Bedford and Clarke, 1972; Hruban et al., 1966; Graham and Kuizenga, 1945;…...

    [...]

  • ...…1966; Schafer and Bowles, 1985; Taylor et al., 1964; Hoshino, 1940; Schweinsberg et al., 1986; MacEwen, 1986; Abramovici and Rachmuth-Roizman, 1983; Nishihara et al., 2000; SCCNFP, 2002; WHO, 2000; Kreis et al., 1971; Bedford and Clarke, 1972; Hruban et al., 1966; Graham and Kuizenga, 1945;…...

    [...]

  • ...…1940; Miller et al., 1983; Duncan and Jarvis, 1943; Foulon et al., 2005; deJouffrey et al., 2004; Jost, 1953; MacMillan, 1973; Duraiswami, 1954; Nishihara et al., 2000; Blair et al., 2000; Teuchy et al., 1971; Bray et al., 1951, 1958; McCloskey et al., 1986a, 1986b; RIFM, 1987b; LeBel et al.,…...

    [...]

  • ...…et al., 1995; Kimmel et al., 1971; Ashby et al., 1997; Bedford and Clarke, 1971; Benton et al., 1955; Schafer and Bowles, 1985; Dawson et al., 1996; Nishihara et al., 2000; Picard et al., 2001; Kolle et al., 2010; Minor and Becker, 1971; Verrett et al., 1980; Daston et al., 1995; Peterka et al.,…...

    [...]

  • ...…et al., 1995; Kimmel et al., 1971; Ashby et al., 1997; Bedford and Clarke, 1971; Benton et al., 1955; Schafer and Bowles, 1985; Dawson et al., 1996; Nishihara et al., 2000; Picard et al., 2001; Kolle et al., 2010; Minor and Becker, 1971; Verrett et al., 1980; Daston et al., 1995; Peterka et al.,…...

    [...]

References
More filters
Book•
Our Stolen Future

[...]

Theo Colborn, Dianne Dumanoski, John Peterson Myers
01 Jan 1996
TL;DR: The cause of disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure, is traced to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system.
Abstract: For years, scientists have noticed disruptions in animal breeding cycles, accompanied by increases in birth defects, sexual abnormalities and reproductive failure. Humans are not immune either, with sperm counts dropping by as much as 50% in recent decades and with women seeing a rise in hormone-related cancers, endometriosis and other disorders. This book traces the cause of these aberrations and diseases to the pervasive presence in the environment of chemicals that mimic hormones and trick the reproductive system. The conclusions are as obvious as they are inescapable - unless we make vital changes in the way we manufacture and employ the artefacts of our "good life", there will be no life at all.

917 citations

Related Papers (5)
The Estrogen Receptor Relative Binding Affinities of 188 Natural and Xenochemicals: Structural Diversity of Ligands

[...]

01 Mar 2000-Toxicological Sciences
R. Blair, Hong Fang, William S. Branham, Bruce S. Hass, Stacey L. Dial, Carrie L. Moland, Weida Tong, Leming Shi, Roger Perkins, Daniel M. Sheehan 
The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants.

[...]

01 Oct 1995-Environmental Health Perspectives
Ana M. Soto, Carlos Sonnenschein, Kerrie L. Chung, Mariana F. Fernández, Nicolás Olea, Fátima Olea Serrano 
Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen

[...]

01 Mar 1996-Environmental Toxicology and Chemistry
Edwin J. Routledge, John P. Sumpter
Developmental effects of endocrine-disrupting chemicals in wildlife and humans.

[...]

01 Oct 1993-Environmental Health Perspectives
Theo Colborn, F S vom Saal, Ana M. Soto
A variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic.

[...]

01 Jun 1995-Environmental Health Perspectives
Susan Jobling, Tracey Reynolds, Roger White, Malcolm G. Parker, John P. Sumpter